4,180 results

1. pX330S-3

   Plasmid

   #58779

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFGC-pcoCas9

   Plasmid

   #52256

   Purpose

   A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA

   Depositor

   Jen Sheen

   Insert

   35SPPDK:pcoCas9:NOS cassette
   

   Use

   CRISPR; Plant expression

   Promoter

   constitutive 35SPPDK promoter

   Available Since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-sgRNA EF1Alpha-puro-T2A-BFP

   Plasmid

   #60955

   Purpose

   Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

   Depositor

   Jonathan Weissman

   Article

   Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

   Inserts

   sgGFP-NT2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSANG10-3F-BG4

   Plasmid

   #55756

   Purpose

   Expresses BG4 scFv anti-G-quadruplex antibody in bacteria

   Depositor

   Shankar Balasubramanian

   Article

   Biffi et al Nat Chem. 2013 Mar;5(3):182-6. doi: 10.1

   Insert

   BG4
   

   Tags

   6xHis, pelB leader, and tri-FLAG

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-SFFV-dCas9-BFP-KRAB

   Plasmid

   #46911

   Purpose

   Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain

   Depositor

   Jonathan Weissman, Stanley Qi

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   dCas9-BFP-KRAB fusion
   

   Use

   CRISPR and Lentiviral

   Tags

   2xNLS, BFP, HA, and KRAB domain

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV/SB10

   Plasmid

   #24551

   Depositor

   Perry Hackett

   Article

   Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

   Insert

   Sleeping Beauty
   

   Expression

   Bacterial

   Available Since

   Oct. 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR_pGK_LaG17_synNotch_Gal4VP64

   Plasmid

   #79127

   Purpose

   Lentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch Gal4DBDVP64 Receptor (PGK promoter)

   Depositor

   Wendell Lim

   Article

   Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

   Insert

   LaG17_synNotch_Gal4DBDVP64
   

   Use

   Lentiviral

   Available Since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-MAPPER

   Plasmid

   #117721

   Purpose

   MAPPER Selectively Labels ER-PM Junctions

   Depositor

   Jen Liou

   Article

   Chang et al Cell Rep. 2013 Nov 14;5(3):813-25. doi:

   Insert

   MAPPER
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. 1GFP/RNase H1 D210N

   Plasmid

   #174448

   Purpose

   Express GFP-tagged RNase H1 D210N in E. coli.

   Depositor

   Karlene Cimprich

   Article

   Crossley et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   RNase H1 (RNASEH1 Human)
   

   Tags

   His-tag, GFP

   Expression

   Bacterial

   Mutation

   D210N

   Promoter

   T7 promoter

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAVS1 T2 CRIPR in pX330

    Plasmid

    #72833

    Purpose

    CRISPR/Cas to target the AAVS1 locus in human cells

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    Cas9 and gRNA for targeting the AAVS1 locus in human cells
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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