Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 24,628 results for: Nes

Showing: 2841 - 2880 of 24628 results

  3. SOX9-T2A-NLS-tdTomato-PGK-Puro

    Plasmid
    #89993
    Purpose
    Donor template for generation of SOX9-ntdTomato reporter cell lines
    Depositor
    Timo Otonkoski
    Insert
    SOX9-T2A-NLS-TdTomato-PGK-Puro (SOX9 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    none
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    89993-plasmid-map-sequence-id-180698

    Enlarge

  8. mpCHECK2

    Plasmid
    #167576
    Purpose
    The mpCHECK2 plasmid has the ability to evaluate the effect of polyadenylation signal variants on expression of disease-associated genes.
    Depositor
    Ting Ni
    Article
    Chen et al Genome Res. 2021 Apr 19. pii: gr.270256.
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    167576-plasmid-map-sequence-id-329146

    Enlarge

  14. mCherry-ActA-IRES-puromycin-pLVx-EF1a

    Plasmid
    #177433
    Purpose
    To express mCherry fused to ActA mitochondria targeting sequence. Lentiviral vector used to make cell lines expressing this mitochondria landmark.
    Depositor
    David Andrews
    Insert
    ActA tail anchor (actA Listeria monocytogenes)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177433-plasmid-map-sequence-id-348464

    Enlarge

  16. pV1081

    Plasmid
    #111425
    Purpose
    Solo vector for expression of CaCas9 and sgRNA for CaADE2 mutagenesis - integrates at ENO1
    Depositor
    Gerald Fink
    Article
    Vyas et al Sci Adv. 2015;1(3):e1500248.
    Insert
    CaCas9/sgADE-NT2
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available Since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111425-plasmid-map-sequence-id-212268

    Enlarge

  20. pLVX ALIX-GFP S718/721/E

    Plasmid
    #138584
    Purpose
    Expresses fluorescent ALIX S718/721E in mammalian cells
    Depositor
    Wesley Sundquist
    Insert
    ALIX (PDCD6IP Human)
    Use
    Lentiviral
    Tags
    GFP and h30
    Expression
    Mammalian
    Mutation
    changed serines 718 and 721 to glutamic acids
    Promoter
    TREtight
    Available Since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    138584-plasmid-map-sequence-id-275093

    Enlarge

  21. Venus-79aa-ActA-pEGFP-C1

    Plasmid
    #166763
    Purpose
    To target Venus to the mitochondrial outer membrane. ActA tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.
    Depositor
    David Andrews
    Insert
    ActA tail anchor (Listeria monocytogenes) (actA Listeria monocytogenes, Synthetic)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    166763-plasmid-map-sequence-id-328851

    Enlarge

  22. LP102: pMAGIC (R4-R3) NLS-Sp Cas9-NLS

    Plasmid
    #132927
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2xNLS SpCas9 (nuclease active) for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Insert
    humanized SpCas9
    Use
    CRISPR and Synthetic Biology; Pmagic gateway entr…
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Oct. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    132927-plasmid-map-sequence-id-314451

    Enlarge

  29. pMin1

    Plasmid
    #102959
    Purpose
    Mini-transposon system suitable for sequencing, shuttle mutagenesis and gene fusions
    Depositor
    Rainer Haas
    Article
    Kahrs et al Gene. 1995 Dec 29;167(1-2):53-7.
    Type
    Empty backbone
    Use
    Mini-transposon system suitable for sequencing, s…
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    102959-plasmid-map-sequence-id-199158

    Enlarge

  35. pSLIK DNM1L-V5 neo

    Plasmid
    #220363
    Purpose
    Lentiviral expression vector for an inducible V5-tagged DNM1L construct
    Depositor
    Kevin Janes
    Insert
    DNM1L (DNM1L Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220363-plasmid-map-sequence-id-436888

    Enlarge

  36. pETMCSI-T4P_Y320A

    Plasmid
    #122522
    Purpose
    Expression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesis
    Depositor
    Gottfried Otting
    Article
    Qi et al PLoS One. 2019 Jan 23;14(1):e0211065. do
    Insert
    gp43 - DNA polymerase (43 Escherichia virus T4)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    tyrosine 320 changed to alanine
    Promoter
    T7 promoter
    Available Since
    March 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    122522-plasmid-map-sequence-id-238200

    Enlarge

Showing: 2841 - 2880 of 24628 results