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We narrowed to 3,513 results for: mpo

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Showing: 2841 - 2880 of 3513 results

  1. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(D256A/PAMmut)

    Plasmid
    #176141
    Purpose
    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutation Asp256Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    XRCC1-T2A-PolB (XRCC1, POLB Human)
    Use
    Lentiviral
    Tags
    EGFP and MYC
    Expression
    Mammalian
    Mutation
    Myc-tagged PolB with mutation in Asp256 to Ala, a…
    Promoter
    EF1A
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176141-plasmid-map-sequence-id-353041

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  2. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(K35A/K68A/K72/PAMmut)

    Plasmid
    #176140
    Purpose
    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutations Lys35Ala, Lys68Ala, Lys72Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resis
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    XRCC1-T2A-PolB (XRCC1, POLB Human)
    Use
    Lentiviral
    Tags
    EGFP and MYC
    Expression
    Mammalian
    Mutation
    Myc-tagged PolB with mutations Lys35 to Ala, Lys6…
    Promoter
    EF1A
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176140-plasmid-map-sequence-id-353035

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  3. pLenti-EML4-ALK variant 3a G1202R

    Plasmid
    #183830
    Purpose
    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK G1202R mutation in mammalian cells
    Depositor
    Aaron Hata
    Article
    Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do
    Inserts
    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of EML4 exon 1-6a and ALK exon…
    Expression
    Mammalian
    Mutation
    G1202R
    Promoter
    CMV
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    183830-plasmid-map-sequence-id-360489

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  4. pLenti-EML4-ALK variant 3a L1196M/G1202R

    Plasmid
    #183831
    Purpose
    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK L1196M/G1202R mutation in mammalian cells
    Depositor
    Aaron Hata
    Article
    Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do
    Inserts
    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of EML4 exon 1-6a and ALK exon…
    Expression
    Mammalian
    Mutation
    L1196M/G1202R
    Promoter
    CMV
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183831-plasmid-map-sequence-id-360488

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  5. pLenti-EML4-ALK variant 3a I1171N/D1203N

    Plasmid
    #183833
    Purpose
    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N/D1203N mutation in mammalian cells
    Depositor
    Aaron Hata
    Article
    Shiba-Ishii et al Nat Cancer. 2022 Jun 20. pii: 10.1038/s4
    Inserts
    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of EML4 exon 1-6a and ALK exon…
    Expression
    Mammalian
    Mutation
    I1171N/D1203N
    Promoter
    CMV
    Available Since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    183833-plasmid-map-sequence-id-360485

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  6. pBIG2abc_hsHAUScomplex_FL

    Plasmid
    #202203
    Purpose
    Baculoviral transfer vector to co-express 8 subunits of human augmin complex
    Depositor
    Thomas Surrey
    Article
    Ustinova et al J Cell Sci. 2023 Jun 15;136(12):jcs26109
    Inserts
    HAUS1 (HAUS1 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS3 (HAUS3 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS2 (HAUS2 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS6 (HAUS6 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS7 (HAUS7 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS4 (HAUS4 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS5 (HAUS5 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS8 (HAUS8 codon-optimized form for expression in Trichoplusia ni cells, Human)
    Tags
    -mGFP-TEV-TwinStrep and His-tag (6x)
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available Since
    Sept. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    202203-plasmid-map-sequence-id-407808

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  12. pARSCL212

    Plasmid
    #198638
    Purpose
    Level 0 35S:Ω:Ruby:t35S component
    Depositor
    Shaun Curtin
    Insert
    35S:Ω:Ruby:t35S
    Expression
    Bacterial
    Available Since
    May 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    198638-plasmid-map-sequence-id-393406

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  13. pMSCV-Mic60

    Plasmid
    #191980
    Purpose
    Expression of V5-tagged Mic60, a component of the MICOS complex.
    Depositor
    Luca Scorrano
    Insert
    Mic60-V5
    Use
    Retroviral
    Available Since
    Jan. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    191980-plasmid-map-sequence-id-379609

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  14. PB-NEO-GFP-RFP

    Plasmid
    #157952
    Purpose
    seamless gene editing and component of Piggybac transposase system
    Depositor
    Yan-zhen Bi
    Type
    Empty backbone
    Use
    Mouse Targeting; Gene targeting
    Expression
    Mammalian
    Available Since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    157952-plasmid-map-sequence-id-310668

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  15. pRVdG-4RFP-5premGRASP

    Plasmid
    #52499
    Purpose
    Expresses TagRFP-T and presynaptic mGRASP component
    Depositor
    Ian Wickersham
    Inserts
    TagRFP-T
    presynaptic mGRASP
    Use
    Rabies virus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    52499-plasmid-map-sequence-id-81437

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  16. pRVdG-4BFP2-5postmGRASP

    Plasmid
    #52498
    Purpose
    Expresses mTagBFP2 and postsynaptic mGRASP component
    Depositor
    Ian Wickersham
    Inserts
    mTagBFP2
    postsynaptic mGRASP
    Use
    Rabies virus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    52498-plasmid-map-sequence-id-81435

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  17. pGEM4Z-OMA1

    Plasmid
    #61718
    Purpose
    human Oma1 protease in the E.coli/in vitro translation vector
    Depositor
    Oleh Khalimonchuk
    Insert
    OMA1 (OMA1 Human)
    Expression
    Bacterial
    Available Since
    Dec. 22, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. XLone-Puro eGFP

    Plasmid
    #140027
    Purpose
    Tunable and temporal expression control of nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140027-plasmid-map-sequence-id-275840

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  20. pCMV-Tag4-OMA1

    Plasmid
    #61719
    Purpose
    mammalian expression of human Oma1 with C-terminal FLAG tag
    Depositor
    Oleh Khalimonchuk
    Insert
    OMA1 (OMA1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    61719-plasmid-map-sequence-id-175330

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  21. pJK640

    Plasmid
    #72467
    Purpose
    Encodes Escherichia coli evgA
    Depositor
    T. J. Kappock
    Insert
    response regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72467-plasmid-map-sequence-id-134013

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  22. pJK641

    Plasmid
    #72468
    Purpose
    Encodes Escherichia coli evgA (Plac noncoding)
    Depositor
    T. J. Kappock
    Insert
    response regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
    Expression
    Bacterial
    Promoter
    lac
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72468-plasmid-map-sequence-id-134015

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  23. pJK646

    Plasmid
    #72469
    Purpose
    Encodes Escherichia coli evgA (Plac coding)
    Depositor
    T. J. Kappock
    Insert
    response regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
    Expression
    Bacterial
    Promoter
    none
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72469-plasmid-map-sequence-id-134016

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  24. PKA1284

    Plasmid
    #239367
    Purpose
    GST-Las17 (342-394) minimal component
    Depositor
    Kathryn Ayscough
    Insert
    Las17 (amino acids 342-392)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    fragment corresponding to amino acids 342-392
    Available Since
    July 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    239367-plasmid-map-sequence-id-478746

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  25. XLone-BSD SPI1 P2A mCherry

    Plasmid
    #140031
    Purpose
    Tunable and temporal expression control of SPI1 and mCherry
    Depositor
    Xiaoping Bao
    Insert
    SPI1 (SPI1 Human)
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140031-plasmid-map-sequence-id-275874

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  26. XLone-Puro SPI1 P2A mCherry

    Plasmid
    #140029
    Purpose
    Tunable and temporal expression control of SPI1 and mCherry
    Depositor
    Xiaoping Bao
    Insert
    SPI1 (SPI1 Human)
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140029-plasmid-map-sequence-id-275844

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  28. LO601: pMVP (R4-R3) destabilized firefly luciferase (Luc2P)

    Plasmid
    #132926
    Purpose
    pMVP R4-R3 entry plasmid, contains destabilized Firefly Luciferase (Luc2P) for 3- or 4-component MultiSite Gateway Pro assembly
    Depositor
    Christopher Newgard
    Insert
    Luc2P
    Use
    Luciferase and Synthetic Biology; Pmvp gateway en…
    Tags
    PEST destabilization domain
    Available Since
    Oct. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    132926-plasmid-map-sequence-id-314396

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  29. XLone-eGFP NLS (no drug resistant gene)

    Plasmid
    #140032
    Purpose
    Tunable and temporal expression control of eGFP (or gene of interest) with constitutive nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140032-plasmid-map-sequence-id-275878

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  33. pOpen-RNAse A

    Plasmid
    #165565
    Purpose
    Ribonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    RNAse A
    Use
    Synthetic Biology
    Available Since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165565-plasmid-map-sequence-id-340093

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  35. pDRGFP

    Plasmid
    #26475
    Purpose
    In vivo homologous recombination substrate composed of two differentially mutated GFP genes oriented as direct repeats and separated by a drug selection marker
    Depositor
    Maria Jasin
    Article
    Pierce et al Genes Dev. 1999 Oct 15. 13(20):2633-8.
    Insert
    DRGFP
    Expression
    Mammalian
    Available Since
    Oct. 18, 2010
    Availability
    Academic Institutions and Nonprofits only
    26475-plasmid-map-sequence-id-432928

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  36. pAAV2-CAGIG-WGA-mCherry

    Plasmid
    #224119
    Purpose
    To express the fusion protein composed of the Wheat germ agluttinin to the fluorescent protein mCherry. Labels the Golgi apparatus, ER and the secretory pathway. Secreted form, labels Lysosomes.
    Depositor
    Pantelis Tsoulfas
    Insert
    WGA-mCherry
    Use
    AAV
    Tags
    WGA and mCherry
    Expression
    Mammalian
    Mutation
    All mutations related to the WGA in this construc…
    Promoter
    CAGIG
    Available Since
    Sept. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    224119-plasmid-map-sequence-id-446520

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  38. XLone-BSD NFIL3 P2A eGFP NLS

    Plasmid
    #140030
    Purpose
    Tunable and temporal expression control of NFIL3 and nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    NFIL3 (NFIL3 Synthetic, Human)
    Use
    Synthetic Biology
    Tags
    eGFP
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140030-plasmid-map-sequence-id-275866

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  40. {Ud}RpL14.Dm

    Plasmid
    #53220
    Purpose
    {Ud} is composed of two genes; (1) a UAS-RpL14.dsRNA targeting RNAi to a haploinsufficient gene RpL14 and (2) an RNAi insensitive RpL14 rescue
    Depositor
    Diethard Tautz
    Insert
    RpL14 (RpL14 Fly)
    Use
    Attb drosophila melanogaster transformation
    Mutation
    14 synonymous mutations introduced
    Promoter
    endogenous promoter
    Available Since
    Nov. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    53220-plasmid-map-sequence-id-82863

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Showing: 2841 - 2880 of 3513 results