We narrowed to 6,480 results for: poly
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EGFP-mCherry-Ki67
Plasmid#183750PurposeExpresses EGFP-mCherry-Ki67DepositorInsertMKI67 (MKI67 Human)
UseTagsEGFP and mCherryExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-GST
Plasmid#171486PurposeT7 promotor drives in vitro transcription of GST mRNADepositorInsertGST
UsePgehmeTagsExpressionMutationPromoterT7Available sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFAP-Cre
Plasmid#24704DepositorInsertCre
UseTagsExpressionMammalianMutationPromoterGFAP (glial fibrillary acidic protein)Available sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
Ki67 (LR)-mNeonGreen-IRESpuro2
Plasmid#183743PurposeExpresses Ki67 (LR)-mNeonGreenDepositorInsertMKI67 LR domain (MKI67 Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationLR domain of Ki67PromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
ptrc99a-pVhb-tdTER-fdh
Plasmid#215649PurposepBR322 Origin, AmpR, with tdTER and Fdh genes under Vhb promoterDepositorInsertstrans-enoyl-CoA reductase
formate dehydrogenase
UseTagsExpressionBacterialMutationPromoterVhbAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBP-J23100
Plasmid#72963PurposeLevel 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion)DepositorInsertJ23100 promoter
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 WAVE 2
Plasmid#67098PurposeStandard for cell-free expression benchmarking.DepositorInsertWAVE 2 (WASF2 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ki67∆repeats-mNeonGreen-IRESpuro2
Plasmid#183742PurposeExpresses Ki67∆repeats-mNeonGreenDepositorInsertMKI67(lacking repeats) (MKI67 Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationMKI67(lacking repeats)PromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBTRCk-pVhb-maACR
Plasmid#215651PurposepBBR1 Origin, KanR, with maACR gene under Vhb promoterDepositorInsertacyl-CoA reductase
UseTagsMaltose binding protein and His tagExpressionBacterialMutationPromoterVhbAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-pVhb-vfFadBA
Plasmid#215650PurposepACYC Origin, CmR, with vfFadBA gene under Vhb promoterDepositorInsertthiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)
UseTagsExpressionBacterialMutationPromoterVhbAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21a - SodA
Plasmid#89612PurposeExpresses recombinant 6HIS tagged T. cruzi superoxide dismutase A protein in E. coli upon IPTG inductionDepositorInsertSuperoxide dismutase A
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLMB757
Plasmid#83792PurposeFructose biosensor plasmidDepositorInsertRegion upstream RL0489 (frcB) containing all of RL0488 (frcR)
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB713
Plasmid#83785PurposeSalicylic acid biosensor plasmidDepositorInsertRegion upstream RL1329 (salA) containing all of RL1328 (salR)
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a - APX
Plasmid#89618PurposeExpresses recombinant 6HIS tagged T. cruzi ascorbate-dependent tryparedoxin peroxidase protein in E. coli upon IPTG inductionDepositorInsertAscorbate dependent Tryparedoxin peroxidase
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 RUVBL1
Plasmid#67091PurposeStandard for cell-free expression benchmarking.DepositorInsertRUVBL1 (RUVBL1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 IL2
Plasmid#67053PurposeStandard for cell-free expression benchmarking.DepositorInsertIL2 (IL2 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mClover3
Plasmid#171497PurposeT7 promotor drives in vitro transcription of mClover3 mRNADepositorInsertmClover3
UsePgehmeTagsExpressionMutationPromoterT7Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 PDGFRB
Plasmid#67130PurposeStandard for cell-free expression benchmarking.DepositorInsertPDGFRB (PDGFRB Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
H2B-2A-GFP-IRESpuro2
Plasmid#183746PurposeExpresses H2B-2A-GFPDepositorInsertH2B (H2BC16P Human)
UseTagsGFP with 2A protease cleavage site between H2B an…ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a - CPX
Plasmid#89620Purpose[Edit] Expresses recombinant 6HIS tagged T. cruzi cytoplasmic tryparedoxin peroxidase protein in E. coli upon IPTG inductionDepositorInsertCytosolic Tryparedoxin peroxidase
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLMB577
Plasmid#83778PurposePhenylalanine biosensor plasmidDepositorInsertpromoter of RL1860
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 PRKCA
Plasmid#67115PurposeStandard for cell-free expression benchmarking.DepositorInsertPRKCA Protein kinase C alpha type (PRKCA Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLMB686
Plasmid#83780Purposemyo-Inositol biosensor plasmidDepositorInsertupstream region of RL4655 (intA)
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a - SodB
Plasmid#89613PurposeExpresses recombinant 6HIS tagged T. cruzi superoxide dismutase B protein in E. coli upon IPTG inductionDepositorInsertSuperoxide dismutase B
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceApril 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLMB684
Plasmid#83779PurposeXylose biosensor plasmidDepositorInsertupstream region of RL2720 (rbsC)
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 PPARG
Plasmid#67099PurposeStandard for cell-free expression benchmarking.DepositorInsertPPARG (PPARG Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLMB687
Plasmid#83781PurposeSucrose biosensor plasmidDepositorInsertRegion upstream pRL120556 containing all of pRL120555
UseTagsExpressionMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 PSMC1
Plasmid#67087PurposeStandard for cell-free expression benchmarking.DepositorInsertPSMC1 (PSMC1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationE275G, I287V and R294GPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLMB708
Plasmid#83782PurposeTartrate biosensor plasmidDepositorInsertRegion upstream RL0996 containing all of RL0997
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB710
Plasmid#83783PurposeFormate biosensor plasmidDepositorInsertRegion upstream RL4393 (fdsG) containing all of RL4394
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB712
Plasmid#83784PurposeHesperetin biosensor plasmidDepositorInsertRegion upstream pRL100185 (nodA) containing part, but not all of, pRL100184 (nodD)
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB714
Plasmid#83786PurposeC4-dicarboxylates biosensor plasmidDepositorInsertRegion upstream RL3424 (dctA)
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB705
Plasmid#83787PurposeMannitol biosensor plasmidDepositorInsertRegion upstream RL4218 (mtlB) containing all of RL4219
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB717
Plasmid#83788PurposeErythritol biosensor plasmidDepositorInsertRegion upstream pRL90085 containing all of pRL90087
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB737
Plasmid#83790PurposeMalonate biosensor plasmidDepositorInsertRegion upstream RL0990 (matA) containing all of RL0989 (matR)
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB738
Plasmid#83791PurposeGABA biosensor plasmidDepositorInsertRegion upstream RL0102 (gabT) containing all of RL0103
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TCF3
Plasmid#67110PurposeStandard for cell-free expression benchmarking.DepositorInsertTCF3 (TCF3 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationTruncated isoformPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTU2-A-RFP
Plasmid#72954PurposeLevel 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDEDepositorInsertLevel 2 - A site
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 YWHAB
Plasmid#67063PurposeStandard for cell-free expression benchmarking.DepositorInsertYWHAB (YWHAB Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFE-dabAB2
Plasmid#133002PurposepFE carrying both the dabA2 (Uniprot: D0KWS7) and dabB2 genes (Uniprot: D0KWS8)DepositorInsertsdabB2
dabA2
UseTagsExpressionBacterialMutationPromotertetRAvailable sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a - NDH
Plasmid#89626PurposeExpresses recombinant 6HIS tagged T. cruzi NADP-dependent oxidoreductase in E. coli upon IPTG inductionDepositorInsertNAD(P)-dependent oxidoreductase
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceApril 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBz106_CsoS2_MR
Plasmid#140843PurposeHis-tagged CsoS2 Middle Region (MR), pET-14b backboneDepositorInsertCsoS2 Middle Region (MR)
UseTagsHexahistidine tagExpressionBacterialMutationonly amino acids 257-603PromoterT7Available sinceJune 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz75_CB_operon_CsoS2_N3-N4
Plasmid#140857PurposeSame as pLz37 but with CsoS2 truncated such that NTD repeats N1 and N2 are removedDepositorInsertCbbL, CbbS, CsoS2 (delN1-N2), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
UseTagsExpressionBacterialMutationdeleted amino acids 1-109 of CsoS2PromoterAvailable sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz77_CB_operon_CsoS2_N3
Plasmid#140859PurposeSame as pLz37 but with CsoS2 discontinuously truncated such that repeats N1, N2, and N4 are removedDepositorInsertCbbL, CbbS, CsoS2 (delN1-N2, N4), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
UseTagsExpressionBacterialMutationdeleted amino acids 1-108 and 220-235 of CsoS2PromoterAvailable sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz78_CB_operon_CsoS2_delNTD
Plasmid#140860PurposeSame as pLz37 but with CsoS2 truncated to remove the entire NTD (i.e. N1, N2, N3, and N4 are removed)DepositorInsertCbbL, CbbS, CsoS2 (delNTD), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
UseTagsExpressionBacterialMutationdeleted amino acids 1-235 of CsoS2PromoterAvailable sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz76_CB_operon_CsoS2_N4
Plasmid#140858PurposeSame as pLz37 but with CsoS2 truncated such that NTD repeats N1, N2, and N3 are removedDepositorInsertCbbL, CbbS, CsoS2 (delN1-N3), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
UseTagsExpressionBacterialMutationdeleted amino acids 1-189 of CsoS2PromoterAvailable sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz109_CsoS2_NTD
Plasmid#140842PurposeHis-tagged CsoS2 N-terminal domain (NTD), pET-14b backboneDepositorInsertCsoS2 N-terminal domain (NTD)
UseTagsHexahistidine tagExpressionBacterialMutationdeleted amino acids 262-869PromoterT7Available sinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz110_CsoS2_CTD
Plasmid#140844PurposeHis-tagged CsoS2 C-terminal domain (CTD), pET-14b backboneDepositorInsertCsoS2 C-terminal domain (CTD)
UseTagsHexahistidine tagExpressionBacterialMutationdeleted amino acids 1-594PromoterT7Available sinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA-dabAB2
Plasmid#133012PurposepFA carrying the badabA2 (locus_tag: BAS2958) and badabB2 genes (locus_tag: BAS2959)DepositorInsertsdabB2
dabA2
UseTagsExpressionBacterialMutationPromotertetRAvailable sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only
