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pFRT-TO-RPB1-His K1278R CRres si2,4R

Plasmid

#139406

Purpose

Dox-inducible, K1278R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

Depositor

Jesper Svejstrup

Article

Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

Insert

RPB1 (POLR2A Human)

Tags

6xHis

Expression

Mammalian

Mutation

K1278R

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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R619-M68-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-529)-3C-His8-SBP

Plasmid

#166019

Purpose

mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD) optimized for serology

Depositor

Dominic Esposito

Article

Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

Insert

SARS-CoV-2 RBD (S )

Tags

3C-His8-SBP

Expression

Mammalian

Promoter

CMV51

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-RPB1-His CRres K619,627,642R si2,4R

Plasmid

#139409

Purpose

Dox-inducible, K619, 627, 642R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

Depositor

Jesper Svejstrup

Article

Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

Insert

RPB1 (POLR2A Human)

Tags

6xHis

Expression

Mammalian

Mutation

K619, 627, 642R

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-RPB1-His K751,758,767R CRres si2,4R

Plasmid

#139408

Purpose

Dox-inducible, K751, 758, 767R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

Depositor

Jesper Svejstrup

Article

Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

Insert

RPB1 (POLR2A Human)

Tags

6xHis

Expression

Mammalian

Mutation

K751, 758, 767R

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-RPB1-His K710,719R CRres si2,4R

Plasmid

#139407

Purpose

Dox-inducible, K710, 719R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

Depositor

Jesper Svejstrup

Article

Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

Insert

RPB1 (POLR2A Human)

Tags

6xHis

Expression

Mammalian

Mutation

K710,719R

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pTol2-Fli1-zFUCCI-IRES-mTagBFP2 (JDW 1517)

Plasmid

#242602

Purpose

A Tol2 expression vector containing the fli promoter driving FUCCI to label cell cycle in all blood vessels followed by an IRES-mTagBFP2.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean-zGem-P2A-mCherry-zCdt1, nls-mTagBFP2

Use

Zebrafish expression

Promoter

fli promoter

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-FastFUCCI-IRES-myr-mKate2 (JDW 1503)

Plasmid

#242598

Purpose

A CAGGS driven expression vector containing FastFUCCI to label cell cycle state followed by an IRES and a membrane targeted mKate2

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

CAGGS

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-FastFUCCI-IRES-myr-mTagBFP (JDW 1502)

Plasmid

#242597

Purpose

A CAGGS driven expression vector containing FastFUCCI to label cell cycle state followed by an IRES and a membrane targeted mTagBFP2

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

CAGGS

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-BLAST-CMV-LiCre-WPRE (JDW 1495)

Plasmid

#242594

Purpose

Lentiviral vector with a CMV promoter driving a light inducible cre (LiCre).

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LiCre

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-hs-miR-126 (JDW 1401)

Plasmid

#242564

Purpose

Gateway middle entry clone containing an EGFP fused to the human microRNA 126.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP with artificial intron encoding miR-126 (MIR126 Human)

Use

Gateway subcloning

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-mm-miR-500 (JDW 8)

Plasmid

#242563

Purpose

Gateway middle entry clone containing an EGFP fused to the murine microRNA 500.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

GFP with artificial intron and murine microRNA 500 (Mir500 Mouse)

Use

Gateway subcloning

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Rox-nlsKate5V-stop-Rox-myr-Flag-BFP-NEO (JDW 473)

Plasmid

#242587

Purpose

A CAGGS driven, Dre recombinase dependent switch reporter (nls-mKate2 to MbBFP following Dre recombination).

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

nlsKateV5, MbBPF-FLAG, FRTNeoFRT

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1a-EGFP-hs-miR-126 (JDW 1415)

Plasmid

#242581

Purpose

An EF1a driven expression vector containing an EGFP fused to the human microRNA 126.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP with an intron containing hs-miR-126 (MIR126 Human)

Expression

Mammalian

Promoter

human EF1a

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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p5E-Dll4-F2-E1B-B-Globin (JDW 1366)

Plasmid

#229843

Purpose

A gateway compatible 5' entry clone containing the murine Dll4 F2 arterial specific enhancer upstream of a minimal E1b promoter and intron for arterial and endocardial expression.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Dll4-F2 (exon3)

Use

Gateway entry clone

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-lexPR-nls-GFP-pA (JDW 1316)

Plasmid

#229811

Purpose

A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus (1st generation)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA transactivator-LexA-Operon-nls-GFP

Use

Tol2 based expression vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-lexA-oMDC-pA-lexO-cFos-nlsGFP-pA (JDW 1382)

Plasmid

#229813

Purpose

A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator and a c-fos minimal promoter.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-mODC-LexAOp-cFos

Use

Tol2 based expression vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA-2xIns-fli1ep-zCreI-BFP (JDW 1218)

Plasmid

#229819

Purpose

A gateway compatible Tol2 destination vector containing the fli1a promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

Tol2 destination vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Tet-Off-FLEX-DEST (JDW 1186)

Plasmid

#229820

Purpose

An AAV, tet-off, gateway-compatible destination vector with cre-dependent expression of the insert. EFS driving destablized rTA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

AAV

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-Luciferase-P2A-H2A-mCherry (JDW 1129)

Plasmid

#229823

Purpose

A CAGGS driven luciferase reporter followed by a P2A cleavage peptide and an H2A mCherry cassette for nuclear labeling.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase-P2A-H2A-mCherry

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-DEST-hsp70-zCreI-BFP (JDW 1184)

Plasmid

#229831

Purpose

A gateway compatible Tol2 destination vector containing the hsp70 promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

Tol2 destination vector

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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