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18065-M03-412

Plasmid

#225666

Purpose

Lentivirus protein expression. Control plasmid.

Depositor

Dominic Esposito

Article

Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:

Inserts

dsCopGFP
delCD19 (CD19 Human)

Use

Lentiviral

Tags

3xFLAG

Mutation

C-terminally truncated (aa 1-333 only)

Promoter

WPRE-SV40p and mCMVp

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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R725-X11-566: His6-MBP-tev-Hs.LGALS3

Plasmid

#159699

Purpose

E. coli protein expression of His6-MBP-tev-Hs.LGALS3

Depositor

Dominic Esposito

Article

Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

Insert

His6-MBP-tev-Hs.LGALS3 (LGALS3 Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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R717-X61-566: His6-MBP-tev-Hs.RAF1(52-192)

Plasmid

#159687

Purpose

E. coli protein expression of His6-MBP-tev-Hs.RAF1(52-192)

Depositor

Dominic Esposito

Article

Tran et al Nat Commun. 2021 Feb 19;12(1):1176. doi:

Insert

His6-MBP-tev-Hs.RAF1(52-192) (RAF1 Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R725-X20-566: His6-MBP-tev-Avi-Hs.LGALS3

Plasmid

#159700

Purpose

E. coli protein expression

Depositor

Dominic Esposito

Article

Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

Insert

His6-MBP-tev-Avi-Hs.LGALS3 (LGALS3 Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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R713-M50-303: CMV51p> Halotag-HRasHVR

Plasmid

#159685

Purpose

Mammalian protein expression of HRAS HVR (Hypervariable region)-Halotag fusion

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-HRasHVR (HRAS Human)

Tags

Halotag

Expression

Mammalian

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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R713-M48-303: CMV51p> Halotag-Kras4aHVR

Plasmid

#159684

Purpose

Mammalian protein expression of KRAS4a HVR (Hypervariable region)-Halotag fusion

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-Kras4aHVR (KRAS Human)

Tags

Halotag

Expression

Mammalian

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R703-M80-303: CMV51p> Halotag-Kras4bHVR

Plasmid

#159683

Purpose

Mammalian protein expression of KRAS4b HVR (Hypervariable region)-Halotag fusion

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-Kras4bHVR (KRAS Human)

Tags

Halotag

Expression

Mammalian

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R713-M52-303: CMV51p> Halotag-NRasHVR

Plasmid

#159686

Purpose

Mammalian protein expression of NRAS HVR (Hypervariable region)-Halotag fusion

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-NRasHVR (NRAS Human)

Tags

Halotag

Expression

Mammalian

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R620-M06-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-529)-3C-His8-SBP K417N/E484K/N501Y

Plasmid

#170192

Purpose

Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), triple mutant from B.1.351 variant

Depositor

Dominic Esposito

Article

Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

Insert

SARS-CoV-2 RBD (S SARS-CoV-2)

Tags

3C-His8-SBP

Expression

Mammalian

Mutation

K417N, E484K, N501Y

Promoter

CMV51

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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R620-M04-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(318-529)-3C-His8-SBP N501Y

Plasmid

#170190

Purpose

ammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.7 variant

Depositor

Dominic Esposito

Article

Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

Insert

SARS-CoV-2 RBD (S SARS-CoV-2)

Tags

3C-His8-SBP

Expression

Mammalian

Mutation

N501Y

Promoter

CMV51

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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p3E mCherry-T2A-hsHRAS-G12V-pA (JDW 1188)

Plasmid

#242571

Purpose

Gateway 3' entry clone containing mCherry followed by a T2A cleavage peptide and then human HRAS G12V.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

HRAS-G12V (HRAS Human)

Use

Gateway subcloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-Sun1-2xsfGFP-6xMYC-pA (JDW 694)

Plasmid

#242588

Purpose

A CMV driven expression vector containing a Sun1-2xsfGFP for labeling nuclear envenlope.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Sun1-2xsfGFP-6xMYC (Sun1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pME-V5-mTagBFP2-KRAS4A-WT (JDW 831)

Plasmid

#242568

Purpose

Gateway middle entry clone containing an mTagBFP2 fused human KRAS4A WT.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

KRAS4A (WT) (KRAS Human)

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME-V5-mClover-KRAS4A-WT (JDW 812)

Plasmid

#242566

Purpose

Gateway middle entry clone containing an mClover3 fused human KRAS4A WT.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

KRAS4A (WT) (KRAS Human)

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-lsl-EGFP-nls-mCherry:fli1aep-CreI-zf1-BFP (JDW 1236)

Plasmid

#229816

Purpose

A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with fli1-TagBFP-zCre in the opposite direction.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

loxp-EGFP-stop-lox

Use

Tol2 based expression vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Tet-Off-FLEX-Lifeact-mScarlet-HA-WPRE-pA (JDW 721)

Plasmid

#229826

Purpose

Tet-off, AAV vector for cre dependent expression of Lifeact mScarlet tagged with HA with EFS (minimal EF1a promoter or EF1a short/ EFS) driving destablized tTA (d2tTA)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mScarlet-I

Use

AAV

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Hsp70-H2B-mCerulean-lsl-mScarlet-fli1-zCre-I-BFP (JDW 1233)

Plasmid

#229841

Purpose

A Tol2 based expression vector with the Hsp70 promoter driving a cre dependent switch reporter. Contains an endothelial driven cre

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

loxp-H2B-mCerulean-2xStop-loxP

Use

Cre/Lox; Tol2 based expression vector

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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18065-M04-412

Plasmid

#225667

Purpose

Lentivirus protein expression. Control plasmid.

Depositor

Dominic Esposito

Article

Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:

Inserts

dsmCherry
delCD19 (CD19 Human)

Use

Lentiviral

Tags

3xFLAG

Mutation

C-terminally truncated (aa 1-333 only)

Promoter

WPRE-SV40p and mCMVp

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pME-Actin-Vhh-sfGFP-P2A-1xHA-iRFP-caax (JDW 1216)

Plasmid

#224496

Purpose

Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and iRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-sfGFP-P2A-HA-iRFP-caax

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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R620-M18-303: CMV51p> SARS-CoV-2 S-RBD(319-541)-His6 K417T/E484K/N501Y

Plasmid

#170202

Purpose

Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.28 variant

Depositor

Dominic Esposito

Article

Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

Insert

SARS-CoV-2 RBD (S SARS-CoV-2)

Tags

His6

Expression

Mammalian

Mutation

K417T, E484K, N501Y

Promoter

CMV51

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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