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pFREDusk-AmpR-MCS

Plasmid

#229737

Purpose

For far-red light-induced bacterial expression of gene of interest (AmpR)

Depositor

Heikki Takala

Article

Bohm et al ACS Synth Biol. 2025 Jul 11. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_NAP1-eGFP

Plasmid

#216837

Purpose

For mammalian expression of NAP1-GFP (Internal ref. SMC1638)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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EcAvs1

Plasmid

#188787

Purpose

Avs1 from Escherichia coli NCTC9036

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

EcAvs1

Expression

Bacterial

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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SeAvs1

Plasmid

#188786

Purpose

Avs1 from Salmonella enterica NCTC10718

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

SeAvs1

Expression

Bacterial

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Ec3Avs4

Plasmid

#188798

Purpose

Avs4 from Escherichia coli NCTC11132

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

Ec3Avs4

Expression

Bacterial

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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CcAvs4

Plasmid

#188797

Purpose

Avs4 from Corallococcus coralloides DSM2259

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

CcAvs4

Expression

Bacterial

Promoter

lac

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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Ec2Avs4

Plasmid

#188795

Purpose

Avs4 from Escherichia coli 382_h

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

Ec2Avs4

Expression

Bacterial

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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AcAvs3

Plasmid

#188793

Purpose

Avs3 from Arcobacter cryaerophilus M830A

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

AcAvs3

Expression

Bacterial

Promoter

lac

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTK643

Plasmid

#114703

Purpose

Express NuMA(214-705)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(214-705)-RFP-Nano

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pLG010 (DRT type 2)

Plasmid

#157888

Purpose

Expresses the DRT type 2 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

DRT type 2

Expression

Bacterial

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-HaloTag7-NCOA4

Plasmid

#192806

Purpose

Stable expression of HaloTag7-NCOA4 in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Tags

HaloTag7

Expression

Mammalian

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO SwiChRca-TS-EYFP

Plasmid

#55631

Purpose

Cre-Dependent Chloride Channel

Depositor

Karl Deisseroth

Article

Berndt et al Science. 2014 Apr 25;344(6182):420-4. do

Insert

iC1C2-TS-EYFP

Use

AAV and Cre/Lox

Tags

EYFP

Expression

Mammalian

Mutation

C128A

Promoter

Ef1a

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pLG006 (Ec67 retron)

Plasmid

#157884

Purpose

Expresses the Ec67 retron defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

Ec67 retron

Expression

Bacterial

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLG007 (Ec86 retron)

Plasmid

#157885

Purpose

Expresses the Ec86 retron defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

Ec86 retron

Expression

Bacterial

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-mGFP-NCOA4

Plasmid

#192802

Purpose

Stable expression of mGFP-NCOA4 in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Tags

monomeric EGFP (A206K)

Expression

Mammalian

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 TEV (full-length)

Plasmid

#64276

Purpose

Expresses tobacco etch virus protease (TEV)

Depositor

Xiaokun Shu

Article

To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

Tobacco etch virus (TEV) protease

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pLG005 (Retron-TIR)

Plasmid

#157883

Purpose

Expresses the retron-TIR defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

Retron-TIR

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCXLE-gw

Plasmid

#37626

Purpose

Non-integrating (episomal) expression vector with Gateway cassette

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Type

Empty backbone

Expression

Mammalian

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pLG009 (DRT type 1)

Plasmid

#157887

Purpose

Expresses the DRT type 1 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

DRT type 1 (RT-nitrilase)

Expression

Bacterial

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLG011 (DRT type 3)

Plasmid

#157889

Purpose

Expresses the DRT type 3 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

DRT type 3 (RT + RT)

Expression

Bacterial

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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