We narrowed to 162,094 results for: addgene

1. pLVX-PERK(LD)-EGFP-HOTag3

   Plasmid

   #225678

   Purpose

   Used to generate stable cell lines that express PERK(LD)-EGFP-HOTag3 and to detect the unfolded proteins in the ER lumen.

   Depositor

   Likun Wang

   Article

   Xu et al Cell Rep. 2024 Jun 25;43(6):114358. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. HO-hisG-URA3-hisG-poly-HO

   Plasmid

   #51663

   Purpose

   yeast plasmid for integration of target sequence at HO locus, URA3 selection

   Depositor

   David Stillman

   Article

   Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

   Type

   Empty backbone

   Use

   Yeast integration

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiGuide-tagBFP

   Plasmid

   #217007

   Purpose

   lentiviral plasmid for gRNA cloning with selectable tagBFP expression

   Depositor

   Deepta Bhattacharya

   Article

   D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBabe 12S E1A

   Plasmid

   #18742

   Depositor

   Scott Lowe

   Article

   Samuelson et al Proc Natl Acad Sci U S A. 1997 Oct 28. 9

   Insert

   12S E1A
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSCVII

   Plasmid

   #162750

   Purpose

   MSCV backbone

   Depositor

   Maki Nakayama

   Article

   Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. p-dCas9-HDAC1-Hygro

   Plasmid

   #104409

   Purpose

   transient expression of dCas9-HDAC1 fusion protein

   Depositor

   Stephan Beck

   Insert

   HDAC1 (HDAC1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSADdeltaG-mCherry

   Plasmid

   #32636

   Depositor

   Edward Callaway

   Article

   Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

   Insert

   mCherry
   

   Use

   Rabies virus

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti SpBsmBI sgRNA Hygro

   Plasmid

   #62205

   Purpose

   An empty sgRNA expression vector for expression of sgRNA for Sp Cas9

   Depositor

   Rene Maehr

   Article

   Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. CROPseq-Guide-Zeo

   Plasmid

   #127173

   Purpose

   Entry plasmid for guide cloning

   Depositor

   Paul Blainey

   Article

   Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNR220

    Plasmid

    #79541

    Purpose

    Input plasmid: expresses three serine recombinases (TP901, BxbI, and A118) downstream of three inducible promoters.

    Depositor

    Timothy Lu

    Article

    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

    Inserts

    TP901
    BxbI
    A118
    

    Use

    Tags

    His tag and LAA degradation tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pInducer20_EGFP

    Plasmid

    #228878

    Purpose

    Tet-inducible lentiviral vector for expression of optimized GFP

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-Clover-HIF-1alpha Wildtype

    Plasmid

    #163365

    Purpose

    Express HA- and Clover-tagged HIF-1alpha wildtype plasmid

    Depositor

    Violaine Sée

    Insert

    Hypoxia Inducible Factor 1 alpha (HIF1A Human)
    

    Use

    Tags

    HA- and Clover-tagged

    Expression

    Mammalian

    Mutation

    Wildtype

    Promoter

    CMV Promoter

    Available since

    Jan. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTec_mCherry

    Plasmid

    #229145

    Purpose

    Promoter strength evaluation of Ptec

    Depositor

    Shrikrishnan Sankaran

    Article

    Blanch-Asensio et al Microb Cell Fact. 2024 Feb 7;23(1):42. d

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    No

    Promoter

    Ptec

    Available since

    Jan. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-PERK(LD)-EGFP

    Plasmid

    #225680

    Purpose

    Used to generate stable cell lines that express PERK(LD)-EGFP. This plasmid serves as a control for pLVX-PERK(LD)-EGFP-HOTag3.

    Depositor

    Likun Wang

    Article

    Xu et al Cell Rep. 2024 Jun 25;43(6):114358. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHdzCas9-KRAB

    Plasmid

    #92341

    Purpose

    Expression dzCas9-KRAB in microalgae. CRISPR/Cas based plant genome editing and gene regulation, Hyg resistance

    Depositor

    I-Son Ng

    Article

    Kao et al Bioresour Technol. 2017 May 4. pii: S096

    Type

    Empty backbone

    Use

    CRISPR; Plant expression

    Tags

    KRAB

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKL11-GCaMP6f bb100

    Plasmid

    #158983

    Purpose

    Constitutive expression (sigma 70 promoter) of GCaMP6f fluorophore in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

    Insert

    GCaMP6f
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    codon optimized for e. coli from Addgene#100833

    Promoter

    Bba_J23100 (sigma 70)

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. HECTD1

    Plasmid

    #32860

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HECTD1 (HECTD1 Human)
    

    Use

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    amino acids 1879-1966 only

    Promoter

    Available since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pAAV-CMV-FLEX-SaCas9-U6-sgHtr2a

    Plasmid

    #159908

    Purpose

    Mutagenesis of Htr2a

    Depositor

    Larry Zweifel

    Insert

    Htr2a (Htr2a Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pN3-Sp3FL

    Plasmid

    #24541

    Depositor

    Guntram Suske

    Article

    Sapetschnig et al J Biol Chem. 2004 Oct 1. 279(40):42095-1

    Insert

    transcription factor Sp3 (SP3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pLenti-dCas9-KRAB-gRNA-TRE-blast

    Plasmid

    #201152

    Purpose

    Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Inserts

    dCas9-KRAB
    gRNA-TRE
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mutation

    gRNA sequence: TACGTTCTCTATCACTGAT

    Promoter

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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