Addgene

We narrowed to 14,720 results for: nans

Showing: 1441 - 1460 of 14720 results

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pCS2-FAK0

Plasmid

#59128

Purpose

Cloning vector for FAK fret sensor without helix

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Use

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 12, 2014

Availability

Academic Institutions and Nonprofits only

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pNOC-stacked-aequorin

Plasmid

#101010

Purpose

Construct for expression of aequorin, calcium sensitive luciferase reporter and hygromycin resistance gene by a bidirectional promoter

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Tags

Expression

Mutation

Promoter

Ribi

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pUC-TALO8

Plasmid

#83409

Purpose

TRP1-ARS1-8xLacO (TALO8) minichromosome system for histone purification in yeast

Depositor

Toshi Tsukiyama

Article

Unnikrishnan et al Nat Struct Mol Biol. 2010 Apr;17(4):430-

Insert

TRP-ARS-8xLacO

Use

Tags

Expression

Yeast

Mutation

Please see depositor comments below

Promoter

TRP1

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pMSC130

Plasmid

#216364

Purpose

xhNup98-Nb3i Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup98-Nb3i

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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LaM8-AK74

Plasmid

#159594

Purpose

Opto-Nanobody

Depositor

Jared Toettcher, Jose Avalos

Article

Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

Insert

LaM8 with AsLOV2 at AK74

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pSA110

Plasmid

#59784

Purpose

drives mCherry in C. elegans intestinal cells

Depositor

Jeremy Nance

Article

Armenti et al Development. 2014 Dec;141(23):4640-7. do

Inserts

elt-2 promoter (elt-2 Nematode)
mCherry (worm)
unc-54 3' UTR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pBabe puro RalB S28N

Plasmid

#19722

Depositor

Channing Der

Article

Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Insert

V-ral simian leukemia viral oncogene homolog B (RALB Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

S28N for dominant negative function.

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pCDNA-FRT-FAK10

Plasmid

#59119

Purpose

FAK fret sensor with 10 nm helix

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Use

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pMito-GBPen

Plasmid

#128269

Purpose

Expression of mitochondrially targeted GBPen (GFP Nanobody)

Depositor

Stephen Royle

Article

Kuey et al J Cell Sci. 2019 Nov 6;132(21). pii: jcs

Insert

Mito-GBPen

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAF124

Plasmid

#222372

Purpose

Recombinant human IgG1 antibody (3D5) that binds to 6xHis at the C-terminus of proteins

Depositor

Andrew S. Flies

Article

Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

Insert

IGHG

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-csGBP

Plasmid

#108891

Purpose

Multisite gateway vector for 3' tagging with a conditionally stable nanobody against GFP

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

csGBP

Use

Multisite gateway

Tags

Expression

Mutation

Promoter

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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LaM8-GG15-irFP

Plasmid

#159591

Purpose

Opto-Nanobody

Depositor

Jared Toettcher, Jose Avalos

Article

Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

Insert

LaM8 with AsLOV2 at GG15

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pSB_init-Sb44

Plasmid

#159421

Purpose

Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb44

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

Sb44

Use

Tags

c-Myc and poly-His

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hAIM2-T7

Plasmid

#51538

Purpose

Express T7-tagged hAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pSA120

Plasmid

#59789

Purpose

C. elegans heat-shock driven ZIF-1 and mCherry co-expressed in the same operon

Depositor

Jeremy Nance

Article

Armenti et al Development. 2014 Dec;141(23):4640-7. do

Inserts

hsp-16.41 promoter
zif-1 coding
gpd-2/gpd-3 intergenic sequence
mCherry (worm)
unc-54 3' UTR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pXJ40-GFP-iBox-PAK4cat

Plasmid

#190179

Purpose

expresses GFP-tagged iBox-PAK4cat

Depositor

Bianxiao Cui

Article

Li et al Nano Lett. 2019 Oct 9;19(10):6955-6963.

Insert

GFP-iBox-PAK4cat

Use

Tags

Flag and GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA-FRT-FAK0

Plasmid

#45047

Purpose

FAK fret sensor without helix

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Use

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pSB_init_Sb68

Plasmid

#159423

Purpose

Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb68

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

Sb68

Use

Tags

c-Myc and poly-His

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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LaM8-GG15

Plasmid

#159593

Purpose

Opto-Nanobody

Depositor

Jared Toettcher, Jose Avalos

Article

Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

Insert

LaM8 with AsLOV2 at GG15

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pre_II_CS3_7560_v1

Plasmid

#126853

Purpose

7560 b scaffold from compatibility group 3, made by method II

Depositor

Hendrik Dietz

Article

Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

Insert

II_CS3_7560_v1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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