Skip to main content
Addgene
Showing: 2881 - 2920 of 8320 results

  1. pHH0103 C20orf67 WW domain

    Plasmid
    #104273
    Purpose
    Bacterial expression of WW domain from C20orf67
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    C20orf67 WW domain (PCIF1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104273-plasmid-map-sequence-id-203480

    Enlarge

  2. LCB7_crtYB

    Plasmid
    #140232
    Purpose
    Expression of gene coding lycopene bifunctional β-cyclase for production beta-carotene from lycopene
    Depositor
    Kang Zhou
    Insert
    phytoene-beta carotene synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    K307E
    Promoter
    pthrC3
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    140232-plasmid-map-sequence-id-288146

    Enlarge

  3. pJK367

    Plasmid
    #72406
    Purpose
    Produces Acetobacter aceti 1023 Est06
    Depositor
    T. J. Kappock
    Insert
    lipase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    lacks Met1 and Thr2 in annotated (v1) sequence
    Promoter
    T7
    Available since
    Feb. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    72406-plasmid-map-sequence-id-134674

    Enlarge

  4. pHH0103 WWP2 WW domain #1

    Plasmid
    #104212
    Purpose
    Bacterial expression of WW domain #1 from WWP2
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    WWP2 WW domain #1 (WWP2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    March 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    104212-plasmid-map-sequence-id-204807

    Enlarge

  5. pHH0103 WWP2 WW domain #3

    Plasmid
    #104214
    Purpose
    Bacterial expression of WW domain #3 from WWP2
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    WWP2 WW domain #3 (WWP2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    104214-plasmid-map-sequence-id-204291

    Enlarge

  6. pHH0103 WWP2 WW domain #2

    Plasmid
    #104213
    Purpose
    Bacterial expression of WW domain #2 from WWP2
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    WWP2 WW domain #2 (WWP2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    104213-plasmid-map-sequence-id-204203

    Enlarge

  7. pHH0103 WWP2 WW domain #4

    Plasmid
    #104215
    Purpose
    Bacterial expression of WW domain #4 from WWP2
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    WWP2 WW domain #4 (WWP2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    104215-plasmid-map-sequence-id-204195

    Enlarge

  8. pAAV-CAG-FLEX-splitTVA-EGFP

    Plasmid
    #59332
    Purpose
    Expresses splitTVA-P2A-EGFP
    Depositor
    Ian Wickersham
    Insert
    splitTVA950-P2A-EGFP
    Use
    AAV and Cre/Lox
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Sept. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    59332-plasmid-map-sequence-id-92028

    Enlarge

  9. pFastBacI/MDR3cysless

    Plasmid
    #74400
    Purpose
    expression of MDR3 in insect cells
    Depositor
    Robert Nakamoto
    Insert
    MDR3 (Abcb1a Mouse)
    Use
    Tags
    10His
    Expression
    Insect
    Mutation
    all Cys to Ala
    Promoter
    Polyhedrin
    Available since
    March 2, 2018
    Availability
    Academic Institutions and Nonprofits only

  10. pX330 Mouse 3' Hp1a gRNA

    Plasmid
    #127898
    Purpose
    WT Cas9 Vector targeting the 3' end of the mouse Hp1a gene
    Depositor
    Mark Groudine
    Insert
    gRNA/Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127898-plasmid-map-sequence-id-251076

    Enlarge

  11. pSB700-ACTC1-Puro

    Plasmid
    #128324
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)
    Depositor
    Alejandro Chavez
    Insert
    gRNA against human ACTC1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128324-plasmid-map-sequence-id-254244

    Enlarge

  12. p(+)MV-NSE-FlagrP-3g_haP-F497A-3p

    Plasmid
    #58799
    Purpose
    Expresses recombinant measles virus with duplicated P gene. wt P gene and P-F497A gene are tagged with Flag and HA peptides and si1 (siGFP) and si2 (siP) target sequences, respectively.
    Depositor
    Branka Horvat
    Insert
    Measles virus antigenome
    Use
    Tags
    Flag and Ha
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    58799-plasmid-map-sequence-id-91094

    Enlarge

  13. pExp-IF2

    Plasmid
    #112567
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and IF2 (domain I of translation initiation factor IF-2 from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-IF2 and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112567-plasmid-map-sequence-id-217215

    Enlarge

  14. pAG472 H2B-iFAST

    Plasmid
    #130818
    Purpose
    Expresses H2B-iFAST in mammalian cells (nuclear labeling)
    Depositor
    Arnaud Gautier
    Insert
    H2B-iFAST
    Use
    Tags
    myc-tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    130818-plasmid-map-sequence-id-254956

    Enlarge

  15. pJK402

    Plasmid
    #72415
    Purpose
    Produces Acetobacter aceti 1023 Est03, N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    4-hydroxybenzoyl-CoA thioesterase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72415-plasmid-map-sequence-id-135527

    Enlarge

  16. pJK580

    Plasmid
    #71694
    Purpose
    Produces Acetobacter aceti 1023 thioredoxin with a C-terminal His6 tag (AaTrxAH6)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71694-plasmid-map-sequence-id-134489

    Enlarge

  17. pLIX403-EYFP-ccdB-EBFP2

    Plasmid
    #158553
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158553-plasmid-map-sequence-id-309848

    Enlarge

  18. pTXB3-Flag PLK1 T210D

    Plasmid
    #68272
    Purpose
    bacterial expression of Plk1 T210D mutant
    Depositor
    Stefano Ferrari
    Insert
    PLK1 (PLK1 Human)
    Use
    Tags
    Flag and Mxe intein/chitin binding domain
    Expression
    Bacterial
    Mutation
    T210D
    Promoter
    T7
    Available since
    Aug. 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. pCAGGS-S-P1-FLAG

    Plasmid
    #177095
    Purpose
    P1 variant S-protein with c-terminal FLAG tag
    Depositor
    Daniel Conway
    Insert
    S-protein P1 (S SARS-CoV-2)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    177095-plasmid-map-sequence-id-345528

    Enlarge

  20. Paxillin_PSCFP2_ mEOS2

    Plasmid
    #114192
    Purpose
    studying focal adhesion proteins by superresolution microscopy (PALM) with dual labelling
    Depositor
    Aleksandra Radenovic
    Insert
    Paxillin (PXN Chicken)
    Use
    Tags
    PS-CFP2 and mEos2
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    Oct. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    114192-plasmid-map-sequence-id-225613

    Enlarge

  21. pLV V5-Sun1

    Plasmid
    #175113
    Purpose
    Lentiviral expression of V5-tagged mouse Sun1
    Depositor
    Larry Gerace
    Insert
    Sun1 (Sun1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    175113-plasmid-map-sequence-id-351771

    Enlarge

  22. pBS3ndd

    Plasmid
    #97354
    Purpose
    Low background cloning vector for conventional or homologous recombination cloning with sticky blunt or TA compatible ends, kanamycin resistant
    Depositor
    Jamorn Somana
    Insert
    Nucleoid Disruptive Domain
    Use
    Cloning
    Tags
    Expression
    Bacterial
    Mutation
    changed methionine 1 to isoleucine
    Promoter
    E. coli lacZ promoter
    Available since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    97354-plasmid-map-sequence-id-191848

    Enlarge

  23. Clim-DC1-mCherry-H2B-Venus

    Plasmid
    #129559
    Purpose
    nuclear marker
    Depositor
    Inaki Ruiz-Trillo
    Insert
    mcherry and Histone 2B fused to venus
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    129559-plasmid-map-sequence-id-258976

    Enlarge

  24. 4ROJ

    Plasmid
    #64661
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    VAV2:JMC092-C08:C231327 (VAV2 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    May 1, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  25. pORTMAGE302A

    Plasmid
    #128970
    Purpose
    Broad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with high temperature induction
    Depositor
    George Church
    Inserts
    PapRecT
    mutL E32K
    Use
    Tags
    Expression
    Bacterial
    Mutation
    E32K
    Promoter
    cI-pL
    Available since
    Aug. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    128970-plasmid-map-sequence-id-252505

    Enlarge

  26. HC080

    Plasmid
    #59545
    Purpose
    Expresses yeast Atg13 under the endogenous promoter with a GFP N-terminal tag
    Depositor
    Daniel Klionsky
    Insert
    ATG13 (ATG13 Budding Yeast)
    Use
    Tags
    GFP
    Expression
    Bacterial and Yeast
    Mutation
    AvrII site created after start codon
    Promoter
    ATG13
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  27. PB CAG-R-3xSV40pA-R-td-sfGFP

    Plasmid
    #177150
    Purpose
    CAG tdGFP dre/rox reporter piggyBac transposon (3x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Dre / rox ; piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    177150-plasmid-map-sequence-id-348857

    Enlarge

  28. Abr in pAcG2T

    Plasmid
    #38162
    Depositor
    Nora Heisterkamp
    Insert
    ABR (ABR Human)
    Use
    Tags
    GST
    Expression
    Insect
    Mutation
    Promoter
    polyhedron
    Available since
    Aug. 27, 2012
    Availability
    Academic Institutions and Nonprofits only

  29. U6-Lin28aIR

    Plasmid
    #120530
    Purpose
    dsRNA expression
    Depositor
    Petr Svoboda
    Insert
    Lin28 (Lin28a Mouse)
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    120530-plasmid-map-sequence-id-233972

    Enlarge

  30. pRK GFP Vinculin

    Plasmid
    #50531
    Depositor
    Kenneth Yamada
    Insert
    Vinculin (VCL Chicken)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  31. pAAV-synP-FLEX-EGFP-B19G

    Plasmid
    #59333
    Purpose
    Expresses EGFP-P2A-B19G
    Depositor
    Ian Wickersham
    Insert
    EGFP-P2A-B19G
    Use
    AAV and Cre/Lox
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin-1
    Available since
    Sept. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    59333-plasmid-map-sequence-id-92029

    Enlarge

  32. pAc5.1B-EGFP-DmRBM7

    Plasmid
    #66594
    Purpose
    expresses EGFP-tagged RBM7 in Drosophila cells
    Depositor
    Elisa Izaurralde
    Insert
    RBM7 (CG11454 Fly)
    Use
    Drosophila expression
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    Actin 5C
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    66594-plasmid-map-sequence-id-113870

    Enlarge

  33. pTWEEN-miR-224

    Plasmid
    #35683
    Depositor
    George Calin
    Insert
    nsa-mir-224 (MIR224 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  34. pJW1328

    Plasmid
    #69488
    Purpose
    sgRNA(F+E) targeting pha-1 (no Cas9); for co-conversion in C. elegans
    Depositor
    Jordan Ward
    Insert
    sgRNA(F+E) targeting pha-1
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    R07E5.16 U6 promoter
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    69488-plasmid-map-sequence-id-125217

    Enlarge

  36. pX330 Mouse 5' Npm1 gRNA

    Plasmid
    #127900
    Purpose
    WT Cas9 Vector targeting the 5' end of the mouse Npm1 gene
    Depositor
    Mark Groudine
    Insert
    gRNA/Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127900-plasmid-map-sequence-id-249686

    Enlarge

  37. pBEST-PLlacO-1-UTR1-TetR-YbaQ-T500

    Plasmid
    #45778
    Depositor
    Richard Murray
    Insert
    Tetracycline repressor
    Use
    Synthetic Biology
    Tags
    YbaQ degradation tag
    Expression
    Bacterial
    Mutation
    Promoter
    PLlacO-1
    Available since
    July 19, 2013
    Availability
    Academic Institutions and Nonprofits only
    45778-plasmid-map-sequence-id-64250

    Enlarge

  38. p(+)MV-NSE-FlagrP-3g_haP-M502-3p

    Plasmid
    #58798
    Purpose
    Expresses recombinant measles virus with duplicated P gene tagged with Flag and HA peptides and si1 (siGFP) and si2 (siP) target sequences, respectively.
    Depositor
    Branka Horvat
    Insert
    Measles virus antigenome
    Use
    Tags
    Flag and Ha
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    58798-plasmid-map-sequence-id-91093

    Enlarge

  39. pBEST-OR2-OR1-Pr-UTR1-araC-T500

    Plasmid
    #45786
    Depositor
    Richard Murray
    Insert
    araC
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    OR2-OR1-Pr (bacteriophage Lambda with one mutatio…
    Available since
    July 19, 2013
    Availability
    Academic Institutions and Nonprofits only
    45786-plasmid-map-sequence-id-64257

    Enlarge

  40. pSC49

    Plasmid
    #104823
    Purpose
    CRISPR/Cas9 2xplex gRNA targeting Glyma17g11235 (Dcl4a). Also expresses Cas9 from AtUBQ10 promoter.
    Depositor
    Robert Stupar
    Insert
    Glyma17g11235
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    104823-plasmid-map-sequence-id-203134

    Enlarge

Showing: 2881 - 2920 of 8320 results