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Plasmid#87000Purposeplasmid for cell culture expression of SYNseq componentsDepositorInsertSNAP-Nlg1AB-1xλN(i)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCO489
Plasmid#139732PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, LuzDepositorInsertpMOD_D_AtuMas:NnLuz:trbcSE9
UseLuciferaseTagsExpressionPlantMutationPromoterAtuMASAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK3a_003
Plasmid#123756PurposeEncodes Nuclear import sequence as a Type 3a part to be used in the MTK systemDepositorInsertNLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
IDP431
Plasmid#86999Purposeplasmid for cell culture expression of SYNseq componentsDepositorInsertCLIP-Nrx1B-4xλN(i)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
IDP455
Plasmid#87001Purposeplasmid for cell culture expression of SYNseq componentsDepositorInsertbarcode landing site Gfp-4xboxB
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCaSpeR-4 porcupine (genomic DNA)
Plasmid#37376DepositorInsertporcupine (por Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
P16ΔS:(MCS)-S11
Plasmid#108184PurposeBinary vectors used for the constitutive expression of tripartite split-sfGFP components in plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterP16ΔSAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV_Zdk3-VPR_P2A_dCas9-AsLOVx3-NLSx3
Plasmid#231568PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertAll-in-one LOOMINA construct
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV_dCas9-AsLOV2x3-NLSx3
Plasmid#231567PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertdCas9-AsLOV2x3
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
1993_pCCLsin.PPT.hPGK.KaedeCETN1.PRE
Plasmid#231804PurposeLabel centrosomes with lentivirus by encoding a fusion protein consisting of the photo-convertible protein Kaede fused to Centrin 1 (CETN1), an integral component of centrosomes.DepositorInsertKaede-CETN1 (Cetn1 Mouse)
UseLentiviralTagsKaedeExpressionMammalianMutationPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
apt-CBE-SL3
Plasmid#219940PurposeMammalian expression of apt-CBE-SL3 protein (MCP_16aa-linker_evoAPOBEC1) with P2A-EGFP markerDepositorInsertMCP_16-aa_evo-rA1_2xUGI
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
PYPQ121-DdCBE
Plasmid#218174PurposeExpress plastid DdCBE, composed of TALE, split DddA originated from Burkholderia cenocepacia and UGIsDepositorInsertTALE-Intron-N-DddAtox half (G1397N)-UGI-T2A-TALE-C-DddAtox half (G1397C)-UGI
UsePlastid base editingTagsExpressionPlantMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV:: PDGFR-Cry2olig-FRB
Plasmid#221773PurposeExpresses PDGFR-Cry2olig-FRB component in mammalian cellsDepositorInsertpCMV:: PDGFR-Cry2olig-FRB
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS306-PTDH3-Dimer-1SUMO
Plasmid#190249PurposeExpression dimer-1SUMO component in yeast cellsDepositorInsertdimer-SUMO
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS304-PTDH3-HEXAMER
Plasmid#190251PurposeExpression hexamer component in yeastDepositorInserthexamer
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
ubc-nls-pcp-10xSunTag (SRv1.1)
Plasmid#185798Purposepcp-10xSunTag component for SunRISER SRv1.1DepositorInsertpcp-10xSunTag
UseTagsFactorXa site, HA, and NLSExpressionMammalianMutationPromoterhuman ubiquitin C promoterAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
ubc-nls-pcp-12xSunTag (SRv1.2)
Plasmid#185799Purposepcp-12xSunTag component for SunRISER SRv1.2DepositorInsertpcp-12xSunTag
UseTagsFactorXa site, HA, and NLSExpressionMammalianMutationPromoterhuman ubiquitin C promoterAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNUT2
Plasmid#175745PurposeSingle-component photo-inducible (LOV2-based) nucleolus-targeting tool 2DepositorInsertmCherry-LOV2-NoLS (human nuclear factor-κB inducing kinase)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH53_pE12_hyCal2
Plasmid#160408PurposeGateway entry clone containing postsynaptic HyCal component (HyNaC2) for HySyn systemDepositorInsertpDONR221 hyCal2
UseOtherTagsExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH54_pE12_hyCal7
Plasmid#160409PurposeGateway entry clone containing postsynaptic HyCal component (HyNaC7) for HySyn systemDepositorInsertpDONR221 hyCal7
UseOtherTagsExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH55_pE12_hyCal9
Plasmid#160410PurposeGateway entry clone containing postsynaptic HyCal component (HyNaC9) for HySyn systemDepositorInsertpDONR221 hyCal9
UseOtherTagsExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH120_pE12_HyPep_emGFP
Plasmid#160406PurposeGateway entry clone containing HyPep presynaptic component (Hydra RFamide) for HySynDepositorInsertpDONR221 HyPep emGFP
UseOtherTagsExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH161_pE12_HyPep_mRuby
Plasmid#160407PurposeGateway entry clone containing HyPep presynaptic component (Hydra RFamide) for HySynDepositorInsertpDONR221 HyPep mRuby
UseOtherTagsExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
IDP254
Plasmid#87004Purposeplasmid for cell culture expression of SYNseq componentsDepositorInsertpostCLAP
UseSynthetic BiologyTagsspGFP (1-10)ExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
IDP253
Plasmid#87003Purposeplasmid for cell culture expression of SYNseq componentsDepositorInsertpreCLAP
UseSynthetic BiologyTagsGFP11ExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
IDP379
Plasmid#87006Purposeplasmid for cell culture expression of SYNseq componentsDepositorInsertpostLib-mRFP-4xboxB
UseSynthetic BiologyTagsmRFPExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
IDP378
Plasmid#87005Purposeplasmid for cell culture expression of SYNseq componentsDepositorInsertpreLib-bfp-4xboxB
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ca-FLARE (TF)
Plasmid#92213PurposeTranscription factor component of FLAREDepositorInsertNRX-TM-Nav1.6 -CaMbp(M2)-eLOV-TEVcs(ENLYFQ▲M)-tTA-VP16
UseAAVTagsExpressionMammalianMutationPromotersynapsinAvailable sinceJune 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)IRES GFP
Plasmid#51406PurposeExpresses GFP from internal ribosome entry sequence, cloning componentDepositorInsertIRES GFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-M13-TEV-C-P2A-TdTomato
Plasmid#92391Purposeproduces AAV expressing Cal-light M13-TevC component and TdTomatoDepositorInsertM13-TEV-C-P2A-TdTomato
UseAAV and Synthetic BiologyTagsTdTomatoExpressionMammalianMutationPromoterhuman synapsinAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
P307-FBP_6
Plasmid#139697PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, control pathwayDepositorInsertpLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
P362-FBP_12
Plasmid#139703PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathwayDepositorInsertpSlH4:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtuMas:NhH3H:tAtuMas, p35S:NnHisps:t35S, p35S:NnLuz:AtuOcs
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAD-GamS
Plasmid#99246PurposeExpresses 6xHis-tagged GamS from T7lac promoter in the E.coli Tuner (DE3) strain. See notes below for important strain information.DepositorInsertgamS (with C-terminal 6xHis tag)
UseTags6xHis tagExpressionBacterialMutationPromoterT7lacAvailable sinceOct. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:(MCS)-S11-DI-GFP1–9
Plasmid#108260PurposeBinary vectors used for the inducible expression of dual intein-coupled tripartite split-sfGFP components in plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
optoAKT
Plasmid#207164PurposeLight-inducible expression of optoAKT; for precise temporal control of the AKT signaling pathwayDepositorInsertCRY2-mCherry-AKT(_PH)-2A-CIBN2-GFP-CAAX
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV:: NES-FKBP-iLID-LRP6c
Plasmid#221774PurposeExpresses NES-FKBP-iLID-LRP6c component in mammalian cellsDepositorInsertpCMV:: NES-FKBP-iLID-LRP6c
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-b2AR-eLOV-TEVcs-FLAG-GAL4-V5
Plasmid#104841Purposeb2AR-SPARK TF componentDepositorInsertb2AR-eLOV-TEVcs-FLAG-GAL4-V5
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR SFFVp YFP-PIF-SOS2cat
Plasmid#50851PurposeOptogenetic Ras activation system; cytoplasmic componentDepositorInsertsUseLentiviralTagsYFPExpressionMutationresidues 566–1049PromoterAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
P336-FBP_11
Plasmid#139702PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing LuzDepositorInsertpLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNPGA:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:(MCS)-S11
Plasmid#108179PurposeBinary vectors used for the inducible expression of tripartite split-sfGFP components in plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only