Addgene

We narrowed to 48,478 results for: ANG

Showing: 1441 - 1460 of 48478 results

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pAs-Cpf1-pGL3-U6-sgRNA

Plasmid

#107683

Purpose

Expresses AsCpf1 crRNA in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pST1374-Lb-Cpf1-NLS

Plasmid

#107680

Purpose

Expresses LbCpf1 in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

LbCpf1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-ccdB-boxB-tBE-V5-mA3

Plasmid

#171693

Purpose

Expresses tBE-V5-mA3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

Insert

ccdB-sgRNA scaffold-boxB-tBE-V5-mA3

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 and EF1a

Available since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCgCas9_recT

Plasmid

#153337

Purpose

Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; Kanr

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

Insert

Cas9

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCgsgRNA_crtYf

Plasmid

#153338

Purpose

Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; rep oriVC. glutamicum; pMB1 oriVE. Coli; Pj23119-crRNA targeting crtYf; Spcr

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

Insert

crRNA targeting crtYf

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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Sa-SchCas9

Plasmid

#192137

Purpose

Expresses Sa-SchCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al Adv Sci (Weinh). 2022 Feb;9(4):e2104789.

Insert

Sa-SchCas9

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBRC_double_attB_GFP_donor

Plasmid

#169692

Purpose

cargo vector for the phiC31 system with Psur5:GFP, cb_unc-119, and Gateway R1R2 site (for gene of interest insertion)

Depositor

John Wang

Article

Yang et al Genetics. 2022 Feb 4;220(2):iyab206. doi

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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Sha3Cas9

Plasmid

#192136

Purpose

Expresses Sha3Cas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al Adv Sci (Weinh). 2022 Feb;9(4):e2104789.

Insert

Sha3Cas9

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-dCpf1-BE

Plasmid

#107685

Purpose

Expresses dLbCpf1-BE in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

dLbCpf1-BE

Use

Tags

Expression

Mammalian

Mutation

D832A/E925A/D1148A

Promoter

CMV

Available since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-mCamk2a-EGFP

Plasmid

#153197

Purpose

Mouse Camk2a (mCamk2a) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-hSyn1-EGFP

Plasmid

#153205

Purpose

Human synapsin-1 (hSyn1) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-mSyn1-EGFP

Plasmid

#153187

Purpose

Mouse synapsin-1 (mSyn1) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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cpLID-micro

Plasmid

#168995

Purpose

circularly permuted LOV2 based optical dimerization system (ssrA-cpLOV2 and sspB)

Depositor

Yubin Zhou

Article

He et al Nat Chem Biol. 2021 Aug;17(8):915-923. d

Insert

mCh-sspB-T2A-cpLID-CAAX

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-CAG-EGFP

Plasmid

#153186

Purpose

CAG promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-ECC-Biosensor

Plasmid

#140832

Purpose

Encoding the CAG promoter based eccDNA biosensor

Depositor

Yonglun Luo

Article

Moller et al Nucleic Acids Res. 2018 Dec 14;46(22):e1

Insert

ECC-Biosensor

Use

Piggybac (pb) transposon

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-dCpf1-eBE-YE

Plasmid

#107689

Purpose

Expresses dLbCpf1-eBE-YE in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

dLbCpf1-eBE-YE

Use

Tags

Expression

Mammalian

Mutation

APOBEC1(W90Y/R126E), dLbCpf1(D832A/E925A/D1148A)

Promoter

CMV

Available since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-TRE-ECC-Biosensor

Plasmid

#140833

Purpose

Encoding the TRE promoter based eccDNA biosensor

Depositor

Yonglun Luo

Article

Moller et al Nucleic Acids Res. 2018 Dec 14;46(22):e1

Insert

TRE-ECC Biosensor

Use

Piggybac (pb) transposon

Tags

Expression

Mammalian

Mutation

Promoter

inverse

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-hIsl2-EGFP

Plasmid

#153206

Purpose

Human Isl2 (hIsl2) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-mIsl2-EGPF

Plasmid

#153191

Purpose

Mouse Isl2 (mIsl2) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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ABE9

Plasmid

#194208

Purpose

Mammalian expression of ABE9

Depositor

Dali Li

Article

Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038

Insert

TadA-8e (N108Q/L145T)-nSpCas9

Use

CRISPR

Tags

6*His

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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