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We narrowed to 1,873 results for: FRT

Showing: 1441 - 1460 of 1873 results

  1. pSU312

    Plasmid
    #169694
    Purpose
    Template plasmid for generating C-terminal 1xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.
    Depositor
    Nara Figueroa
    Article
    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98
    Insert
    1xFLAG epitope tag adjacent to FRT-flanked aph (KanR) gene
    Use
    Tags
    1xFLAG tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169694-plasmid-map-sequence-id-334513

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  2. pSU313

    Plasmid
    #169695
    Purpose
    Template plasmid for generating C-terminal 1xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.
    Depositor
    Nara Figueroa
    Article
    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98
    Insert
    1xFLAG epitope tag adjacent to FRT-flanked cat (CamR) gene
    Use
    Tags
    1xFLAG tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169695-plasmid-map-sequence-id-334516

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  4. pADH100-2

    Plasmid
    #90981
    Purpose
    NAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Insert
    NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, FRT, DS_HIS1
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90981-plasmid-map-sequence-id-180508

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  5. pHW1133

    Plasmid
    #229869
    Purpose
    Destination RMCE vector containing FRT::SA::51-bp Artificial exon::3xStop::SL2::SapI Insertion sites (to clone new driver with 3'UTR in slot 5 and SEC in slot 6)::reverse FRT3 to swap driver via RMCE
    Depositor
    Han Wang
    Article
    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available Since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    229869-plasmid-map-sequence-id-460828

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  8. pcDNA5-hygro-eGFP-STOP-HEC1

    Plasmid
    #114026
    Purpose
    Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.
    Depositor
    Geert Kops
    Article
    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1
    Insert
    eGFP-STOP-HEC1 (NDC80 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Start codon of Hec1 is mutated to stop codon
    Promoter
    Available Since
    Sept. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    114026-plasmid-map-sequence-id-221030

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  9. OpenLoopControl

    Plasmid
    #59895
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Does not contain the target containing Vamp3 3′UTR
    Depositor
    Georg Seelig
    Article
    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.
    Insert
    mCherry with intron containing the mouse mir-124–3
    Use
    Tags
    PEST
    Expression
    Mammalian
    Mutation
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available Since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    59895-plasmid-map-sequence-id-108253

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  18. Fireworks GFP[PTC-] (AVA2598)

    Plasmid
    #85445
    Purpose
    Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.
    Depositor
    Joan Steitz
    Article
    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:
    Insert
    tDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
    Use
    Minimal backbone fragment for low-copy bacterial …
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    85445-plasmid-map-sequence-id-171491

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  20. Fireworks RFP[PTC+] (AVA2626)

    Plasmid
    #85444
    Purpose
    Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.
    Depositor
    Joan Steitz
    Article
    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:
    Insert
    tDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-HygromycinR
    Use
    Minimal backbone fragment for low-copy bacterial …
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85444-plasmid-map-sequence-id-171527

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Showing: 1441 - 1460 of 1873 results