Addgene

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mNeonGreen-Sec61β

Plasmid

#169031

Purpose

General ER marker

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Sec61β (SEC61B Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn3L-mNeonGreen

Plasmid

#169034

Purpose

Express human Rtn3L in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rtn3L (RTN3 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-Rab5CA(Q79L)

Plasmid

#35138

Depositor

Sergio Grinstein

Article

Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

Insert

Rab5a (RAB5A Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Constitutively Active- Q79L

Promoter

CMV

Available since

March 20, 2012

Availability

Academic Institutions and Nonprofits only

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mCherry-Rab5DN(S34N)

Plasmid

#35139

Depositor

Sergio Grinstein

Article

Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

Insert

Rab5a (RAB5A Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Dominant Negative- S34N

Promoter

CMV

Available since

March 20, 2012

Availability

Academic Institutions and Nonprofits only

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pCSIIhyg-RGECO1.0

Plasmid

#175813

Purpose

The lentiviral vector for R-GECO1.0, a genetically encoded red fluorescent Ca2+ sensor.

Depositor

Michiyuki Matsuda

Article

Hirota et al Endocrinology. 2022 Mar 1;163(3). pii: 6

Insert

RGECO1.0

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-PKG-hNo.7

Plasmid

#175812

Purpose

The lentiviral vector for PKG #7, a genetically encoded FRET biosensor for monitoring cGMP concentration.

Depositor

Michiyuki Matsuda

Article

Hirota et al Endocrinology. 2022 Mar 1;163(3). pii: 6

Insert

PKG #7

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pDIV297

Plasmid

#204820

Purpose

AMA1 plasmid with amdS selection marker

Depositor

Uffe Mortensen

Article

Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

Insert

amdS

Use

Fungal expression

Tags

Expression

Bacterial

Mutation

two silent mutations (nt177T>C, nt879C>T)

Promoter

Aspergillus nidulans trpC promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pFL-SV40

Plasmid

#115352

Purpose

firefly luciferase (Fluc) reporter gene driven by SV40 promoter

Depositor

Ming-Chih Lai

Article

Lai et al Mol Biol Cell. 2008 Sep;19(9):3847-58. d

Type

Empty backbone

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pPath-Violacein-pT7native

Plasmid

#200002

Purpose

Violacein pathway. pT7-native CDS sequence

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Pathways

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-MCS-TurboID-3xFLAG

Plasmid

#186814

Purpose

CAG promoter-driven expression of BioID2-fused protein (C terminus)

Depositor

Masahito Ikawa

Article

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Type

Empty backbone

Use

Tags

TurboID-3xFLAG

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-3xFLAG-TurboID-MCS

Plasmid

#186813

Purpose

CAG promoter-driven expression of TurboID-fused protein (N terminus)

Depositor

Masahito Ikawa

Article

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Type

Empty backbone

Use

Tags

3xFLAG-TurboID

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Rab5CA (Q79L)

Plasmid

#35140

Depositor

Sergio Grinstein

Article

Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

Insert

Rab5a (RAB5A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Constitutively Active- Q79L

Promoter

CMV

Available since

March 20, 2012

Availability

Academic Institutions and Nonprofits only

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pCAG-3xFLAG-BioID2-MCS

Plasmid

#186811

Purpose

CAG promoter-driven expression of BioID2-fused protein (N terminus)

Depositor

Masahito Ikawa

Article

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Type

Empty backbone

Use

Tags

3xFLAG-BioID2

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Rab5DN(S34N)

Plasmid

#35141

Depositor

Sergio Grinstein

Article

Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

Insert

Rab5a (RAB5A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Dominant Negative- S34N

Promoter

CMV

Available since

March 20, 2012

Availability

Academic Institutions and Nonprofits only
Rtn3S-GFP

Plasmid

#169035

Purpose

Express human Rtn3S in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rtn3S (RTN3 Human)

Use

Tags

acGFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-PHLPP1 N-terminal truncation

Plasmid

#22404

Purpose

Expresses a HA tagged, N-terminal truncation of human PHLPP1 in mammalian cells

Depositor

Alexandra Newton

Article

Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

Insert

PHLPP1 (PHLPP1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pLSL

Plasmid

#51493

Purpose

T-DNA destination plasmid. Contains the bean yellow dwarf virus cis-acting elements (LIR and SIR) in an LIR-SIR-LIR orientation. Contains attR1 and attR2 sites between the LIR and SIR sequence.

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Type

Empty backbone

Use

Plant t-dna plasmid

Tags

Expression

Mutation

Promoter

virion-sense LIR and 2x35S

Available since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-300(HAT-)

Plasmid

#23254

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

p300 (EP300 Human)

Use

Tags

Expression

Mammalian

Mutation

H1415A E1423A Y1424A L1428S Y1430A H1434A

Promoter

Available since

March 15, 2010

Availability

Academic Institutions and Nonprofits only

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TroponinT-GCaMP5-Zeo

Plasmid

#46027

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

GCaMP5-2A-Zeo

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TNNT2

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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pDIV296

Plasmid

#204687

Purpose

AMA1 plasmid with nat1 resistance marker

Depositor

Uffe Mortensen

Article

Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

Insert

nat1

Use

Fungal expression

Tags

Expression

Bacterial

Mutation

Promoter

Aspergillus nidulans trpC promoter

Available since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge