We narrowed to 8,596 results for: Set

1. AAV-CAG-flex-MCS--4x6T

   Plasmid

   #196420

   Purpose

   Empty vector for astrocyte-selective Cre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassette

   Depositor

   Stanley Thomas Carmichael

   Article

   Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

   Type

   Empty backbone

   Use

   AAV and Cre/Lox; Astrocyte-selective

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pv6_CBX7_2xChromo

   Plasmid

   #179396

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_2xChromo

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX7_2xChromo
   

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Mutation

   G113V in flexible linker

   Promoter

   CAGGS

   Available Since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1-Neo-CAG-Flpe-ERT2

   Plasmid

   #68460

   Purpose

   AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and Neo selection gene

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

   Insert

   mammalian-codon-optimized Flpe recombinase fused with ERT2
   

   Use

   Synthetic Biology

   Tags

   ERT2

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDual-IN-SMN1

   Plasmid

   #215721

   Purpose

   dual-color reporter express eGFP constitutively ; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7; expresses SMN1

   Depositor

   Chaolin Zhang

   Article

   Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

   Insert

   SMN1 EXON 7 (SMN1 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPR-Cre stuffer v4

   Plasmid

   #158033

   Purpose

   Lentiviral construct with Cas9, Cre recombinase and U6 driven sgRNA cassette with modified tracr RNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI)

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPFO*

   Plasmid

   #120400

   Purpose

   Expresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.

   Depositor

   Arun Radhakrishnan

   Article

   Das et al Proc Natl Acad Sci U S A. 2013 Jun 25;11

   Insert

   Mutant form of Perfringolysin O
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Amino acids 1-27 are deleted, starts at amino aci…

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMDC32B-AtMIR390a-B/c

   Plasmid

   #51776

   Purpose

   Plant expression vector (2x35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor

   Depositor

   James Carrington

   Article

   Carbonell et al Plant Physiol. 2014 Mar 19.

   Insert

   AtMIR390a-B/c
   

   Use

   Plant expression

   Mutation

   A. thaliana MIR390a precursor sequence including …

   Available Since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)

   Plasmid

   #86421

   Purpose

   N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequence

   Depositor

   Sue Jaspersen

   Article

   Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

   Insert

   CDC42pr-KAR2SS-GFP1-10
   

   Tags

   GFP1-10

   Expression

   Yeast

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

   Plasmid

   #117687

   Purpose

   U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.

   Depositor

   Erik Sontheimer

   Article

   Gao et al Methods Enzymol. 2019;616:365-383. doi:

   Inserts

   Kanamycin cassette
   TetR-P2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and hPGK

   Available Since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pv6_MBD1_MBD

    Plasmid

    #179404

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    MBD1_MBD
    

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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