We narrowed to 16,820 results for: URE

1. FH-TET3-pEF

   Plasmid

   #49446

   Purpose

   This plasmid expresses full-length human TET3.

   Depositor

   Anjana Rao

   Article

   Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

   Insert

   Tet methylcytosine dioxygenase 3 (TET3 Human)
   

   Use

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha

   Available since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. H9 F42A pAcGP67a

   Plasmid

   #41810

   Depositor

   Chris Garcia

   Article

   Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

   Insert

   H9 Super-2 (IL2 Human)
   

   Use

   Tags

   8x His

   Expression

   Insect

   Mutation

   Phenylalanine 42 to Alanine

   Promoter

   Polyhedron

   Available since

   Jan. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

3. AAV-LK03

   Plasmid

   #206512

   Purpose

   AAV packaging vector containing AAV2 rep and LK03 cap and partial AAV2 p5 promoter

   Depositor

   Mark Kay

   Article

   Lisowski et al Nature. 2014 Feb 20;506(7488):382-6. doi

   Insert

   LK03 capsid
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   p5

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFW2500

   Plasmid

   #224213

   Purpose

   Bacteroides genomic editing tool; IPTG inducible-Fncpf1 and recT

   Depositor

   Ophelia Venturelli

   Article

   Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

   Insert

   Fncpf1 and recT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT2/LTR7-GFP

   Plasmid

   #62541

   Purpose

   LTR7-driving reporter

   Depositor

   Zsuzsanna Izsvak

   Article

   Wang et al Nature. 2014 Dec 18;516(7531):405-9. doi

   Insert

   LTR7-GFP
   

   Use

   Sleeping beauty transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR7

   Available since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV-EGFP-Bulged-miR-19

   Plasmid

   #91976

   Purpose

   EGFP reporter cell line with eight bulged miR-19 sites

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   EGFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28-SaCas9

   Plasmid

   #178901

   Purpose

   SaCas9 (Cas9 from Staphylococcus aureus)

   Depositor

   Hendrik Milting

   Article

   Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

   Insert

   SaCas9
   

   Use

   Tags

   His6-Tag, bipartite nuclear localization signal f…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMX-mTAF6

   Plasmid

   #44337

   Depositor

   Marc Timmers

   Article

   Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

   Insert

   TAF6
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. piggyFlex

   Plasmid

   #218234

   Purpose

   A piggyBac transposon-based gRNA expression vector, to allow for genomic integration and stable expression of gRNAs. Contains both antibiotic (puromycin) and fluorophore (GFP) markers.

   Depositor

   Jay Shendure

   Article

   Chardon et al bioRxiv [Preprint]. 2023 Mar 28:2023.03.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET151-GyrA

    Plasmid

    #216229

    Purpose

    S. aureus USA300 GyrA Codon-optimized for E. coli

    Depositor

    Eric Brown

    Article

    French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

    Insert

    DNA gyrase
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV hSyn FLEx-OFF hM4Di-mCherry

    Plasmid

    #161579

    Purpose

    To express hM4Di in cells not expressing Cre.

    Depositor

    Robert Malenka, Kevin Beier

    Article

    Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

    Insert

    hM4Di-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    hSyn

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA6-IgK-hFRa(23-234)C11S-th-Fc

    Plasmid

    #177841

    Purpose

    Mammalian Expression of Folate Receptor

    Depositor

    Karsten Melcher

    Article

    Chen et al Nature. 2013 Aug 22;500(7463):486-9. doi

    Insert

    Folate receptor alpha (FOLR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    all 11 C mutated to S

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. L2_UBE2:eG-Lt-CsA

    Plasmid

    #136134

    Purpose

    Plasmid to transform Mp with constitutive promoter marking cell outline with eGFP. HygR

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35S:HygR p5-MpUBE2:eGFP-Lti6b
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. L2_UBE2:mT-N7-CsA

    Plasmid

    #136133

    Purpose

    Plasmid to transform Mp with constitutive promoter driving mTurq to the nuclei. HygR

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35S:HygR p5-MpUBE2:mTurq-N7
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pET-28c(+)-YTHDF3

    Plasmid

    #102276

    Purpose

    For bacterial expression of His-tagged human YTHDF3

    Depositor

    Samie Jaffrey

    Article

    Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

    Insert

    YTHDF3 (YTHDF3 Human)
    

    Use

    Tags

    6X HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV hSyn FLEx-OFF hM3Dq-mCherry

    Plasmid

    #161578

    Purpose

    To express hM3Dq in cells not expressing Cre.

    Depositor

    Robert Malenka, Kevin Beier

    Article

    Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

    Insert

    hM3Dq-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    hSyn

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Prolactin-CD4d3+4-bio

    Plasmid

    #73123

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding Prolactin with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    PRL (PRL Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFRT-TO-DEST

    Plasmid

    #106348

    Purpose

    Destination vector for gateway cloning. Developed for Flp-In T-REx system.

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Type

    Empty backbone

    Use

    Gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. BA31_pU.CAG.SaCas9-D10A.rBGpA

    Plasmid

    #199251

    Purpose

    Expression construct encoding the S. aureus Cas9.D10A nickase (SaCas9.D10A)

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Insert

    SaCas9.D10A nickase
    

    Use

    CRISPR

    Tags

    3XHA epitope, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CAG promoter

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRISPRi

    Plasmid

    #199808

    Purpose

    vector for constitutive CRISPRi-mediated gene knockdown in S. aureus

    Depositor

    Steve Salipante

    Article

    Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.

    Inserts

    dCas9
    sgRNA with GFP cassette
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    P23 and PRAB17 (sgRNA) and SarAP1 (GFP)

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. L1_lacZgRNA-Ck3

    Plasmid

    #136136

    Purpose

    L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screening

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpU6:lacZgRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. L1_Cas9-Ck4

    Plasmid

    #136135

    Purpose

    L1 in position 4, for AtcoCas9 (Puchta) expression in L2 CRISPR vectors

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpEF1a:Cas9_3tNos-35S
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSUMO His6 SUMO MYC3

    Plasmid

    #177848

    Purpose

    Bacterial Expression of MYC3

    Depositor

    Karsten Melcher

    Article

    Zhang et al Nature. 2015 Sep 10;525(7568):269-73. do

    Insert

    MYC3 (MYC3 Mustard Weed)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. L1_lacZgRNA-Ck2

    Plasmid

    #136137

    Purpose

    L1 in position 2, to clone gRNA target using BbsI, lacZ blue-white screening

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpU6:lacZgRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. FLAG-Venus-pLenti6

    Plasmid

    #36948

    Depositor

    William Kaelin

    Article

    Koivunen et al Nature. 2012 Feb 15;483(7390):484-8. doi

    Insert

    Venus (EYFP)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    -F46L/F64L/V68L/M153T/V163A/S175G

    Promoter

    CMV

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEGFP-Map7-fulllength

    Plasmid

    #46076

    Depositor

    Edgar Gomes

    Article

    Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

    Insert

    Map7-fulllength (Map7 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEGFP-N1-human lyn–GFP

    Plasmid

    #35958

    Purpose

    Expresses human lyn protein codon optimized for zebrafish expression.

    Depositor

    Anna Huttenlocher

    Article

    Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

    Insert

    LYN (LYN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    cmv

    Available since

    April 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET24a HAB1 (172-511)

    Plasmid

    #177844

    Purpose

    Bacterial Expression of HAB1

    Depositor

    Karsten Melcher

    Article

    Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

    Insert

    Protein phosphatase 2C 16
    

    Use

    Tags

    6xHis-GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLQ-Pxyl/tet-cas9-Pj23119-sgRNA

    Plasmid

    #92121

    Purpose

    CRISPR-Cas9 based efficient genome editing in S. aureus

    Depositor

    Sheng Yang

    Article

    Liu et al Acta Biochim Biophys Sin (Shanghai). 201

    Insert

    Cas9-Pxyltet-sgRNA-pj23119
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAB078d8

    Plasmid

    #79206

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1+2) luxAB; Ppro1 434cI-SH2ABL1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (OR1+2); Ppro1

    Available since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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