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Showing: 2921 - 2960 of 4784 results

  2. p5E-dr_Unc (JDW 1365)

    Plasmid
    #224482
    Purpose
    Gateway compatible 5' entry clone containing the Zebrafish unc-503 promoter with a minimal c-fos promoter and b-globin intron
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv 2024.07.13.603267
    Insert
    Unc-503 promoter
    Use
    Gateway cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    224482-plasmid-map-sequence-id-447104

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  7. pME-TAEL (JDW 1324)

    Plasmid
    #224508
    Purpose
    Gateway compatible middle entry clone containing TAEL2.0 transcription factor for making binary TAEL system vectors (generating a tissue specific driver)
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv 2024.07.13.603267
    Insert
    TAEL
    Use
    Gateway cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    224508-plasmid-map-sequence-id-446945

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  10. pME iCre-I (JDW 1203)

    Plasmid
    #224520
    Purpose
    A Gateway compatible middle entry clone containing a loxP flanked, self inactivating Cre that contains an intron to prevent recombination in bacteria
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv 2024.07.13.603267
    Insert
    iCre-I
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    224520-plasmid-map-sequence-id-446918

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  15. p3E-WPRE-SV40-pA (JDW 922)

    Plasmid
    #224545
    Purpose
    A Gateway compatible 3' entry clone containing a woodchuck herpes simplex virus regulatory element (WPRE) to stabilize mRNA upstream of an SV40 polyA
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv 2024.07.13.603267
    Insert
    WPRE-SV40-pA
    Use
    Gateway cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    224545-plasmid-map-sequence-id-446720

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  16. pEndo-I-OnuI-TSM

    Plasmid
    #207954
    Purpose
    Expresses a fusion of the homing endonuclease I-OnuI with the thermosensitive L212P VMA1 intein. Endonuclease activity is restricted to lower temperatures.
    Depositor
    David Edgell
    Article
    Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206
    Insert
    Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Leucine 212 changed to proline, leucine 434 in fu…
    Promoter
    Available since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    207954-plasmid-map-sequence-id-412663

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  17. pETSUMO2_Nostoc-bGSDM-WELQ

    Plasmid
    #222100
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Nostoc species with a WELQut protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Nostoc-bGSDM-WELQ ORF
    Use
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222100-plasmid-map-sequence-id-437100

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  23. pBCC086

    Plasmid
    #202213
    Purpose
    Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlum
    Depositor
    Paul Schulze-Lefert
    Article
    Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.
    Insert
    pBCC25, pBCC33, pBCC55, pBCC27, pBCC26, pBCC28, pICH41822 (G5)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    202213-plasmid-map-sequence-id-403467

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  24. pBCC091

    Plasmid
    #202218
    Purpose
    Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlum
    Depositor
    Paul Schulze-Lefert
    Article
    Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.
    Insert
    pBCC25, pBCC30, pBCC55, pBCC27, pBCC26, pBCC28, pICH41822 (G5)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    202218-plasmid-map-sequence-id-403465

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  25. pBCC100

    Plasmid
    #202226
    Purpose
    Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Kanamycin selectable marker and far-red-FP fluorescent marker mPlum
    Depositor
    Paul Schulze-Lefert
    Article
    Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.
    Insert
    pBCC25, pBCC32, pBCC55, pBCC27, pBCC26, pBCC28, pICH41822 (G5)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    202226-plasmid-map-sequence-id-403464

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Showing: 2921 - 2960 of 4784 results