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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 6,769 results for: /1000

Showing: 1461 - 1480 of 6769 results

  1. KIF3A560GFP

    Plasmid
    #129769
    Purpose
    Expresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged
    Depositor
    William Hancock
    Insert
    KIF3A (Kif3a Mouse)
    Use
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 359, fused to kinesin-1 starting Al…
    Promoter
    Available since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129769-plasmid-map-sequence-id-263530

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  2. FKBP1B

    Plasmid
    #73241
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    FKBP1B (FKBP1B Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 22, 2016
    Availability
    Academic Institutions and Nonprofits only

  3. MYST1

    Plasmid
    #25180
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MYST1_01 (KAT8 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 9, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. 6PBG

    Plasmid
    #162263
    Purpose
    Bacterial Expression
    Depositor
    Cheryl Arrowsmith
    Insert
    COPA (COPA Human)
    Use
    Baculovirus expresssion
    Tags
    6xHis-TEV
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    Dec. 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    162263-plasmid-map-sequence-id-319824

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  5. R777-E061 Hs.ERBB2

    Plasmid
    #70345
    Purpose
    Gateway ORF clone of human ERBB2 [NM_004448.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    ERBB2 (ERBB2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70345-plasmid-map-sequence-id-125794

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  6. Reduced Expression Alpha-5-Integrin-GFP

    Plasmid
    #30450
    Depositor
    Rick Horwitz
    Insert
    alpha 5 integrin (ITGA5 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    91-544 of enhancer region of CMV promoter removed…
    Promoter
    Available since
    Aug. 29, 2011
    Availability
    Academic Institutions and Nonprofits only

  7. pDBD-R

    Plasmid
    #50571
    Purpose
    Double Ub R-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50572
    Depositor
    Alexander Varshavsky
    Article
    Turner et al Science. 2000 Sep 22;289(5487):2117-20.
    Insert
    DHFR-Ubiquitin-ArgBetaGal-Ubiquitin-DHFR-Ubiquitin
    Use
    Tags
    Expression
    Yeast
    Mutation
    M1R (B-gal)
    Promoter
    Gal1
    Available since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. GFP Cav2.2 HA D665A pcDNA3

    Plasmid
    #206113
    Purpose
    expression of rabbit Cav2.2 with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D665A mutation that allows trafficking to the plasma membrane but channel is not functional
    Depositor
    Annette Dolphin
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    D665A
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    206113-plasmid-map-sequence-id-415392

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  9. pcDNA_DiCre2_59.F2

    Plasmid
    #60209
    Purpose
    Expresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(19-59)
    Depositor
    Jean-Paul Herman
    Insert
    Cre recombinase (CD207 Human, Synthetic, Bacteriophage P1)
    Use
    Tags
    FKBP12(F36V) and Nuclear localisation signal
    Expression
    Mammalian
    Mutation
    FKBP12 has the F36V mutation; amino acids 19-59 o…
    Promoter
    CMV
    Available since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    60209-plasmid-map-sequence-id-96295

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  10. HSPA5A

    Plasmid
    #42447
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HSPA5A (HSPA5 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. mPA-GFP-Caveolin-C-10

    Plasmid
    #57123
    Purpose
    Localization: Membrane, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Insert
    Caveolin (CAV1 Human)
    Use
    Tags
    mPA-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 29, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. DIRAS2

    Plasmid
    #38819
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DIRAS2 (diras2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. mEos-A69V-Actin-7

    Plasmid
    #57334
    Purpose
    Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    Actin (ACTB Human)
    Use
    Tags
    mEos A69V
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. pcDNA3.1/hpa5

    Plasmid
    #59972
    Purpose
    integrin a5 wild-type headpiece expression (a5 beta-propeller and a5 thigh domain)
    Depositor
    Timothy Springer
    Insert
    human integrin a5 headpiece (ITGA5 Human)
    Use
    Tags
    acid coiled-coil and StrepII tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Oct. 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. R777-E006 Hs.AKT3-nostop

    Plasmid
    #70290
    Purpose
    Gateway ORF clone of human AKT3 [NM_005465.4] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    AKT 3 (AKT3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70290-plasmid-map-sequence-id-125739

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  19. PSD-95_Y12E-pTagRFP

    Plasmid
    #52672
    Purpose
    expression of TagRFP-tagged PSD-95_Y12E
    Depositor
    Johannes Hell
    Insert
    PSD-95 (Dlg4 Rat)
    Use
    Tags
    tagRFP
    Expression
    Mammalian
    Mutation
    Y12E
    Promoter
    CMV
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    52672-plasmid-map-sequence-id-81956

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  20. Mouse 5' Srcap AID GFP Puro

    Plasmid
    #127903
    Purpose
    HDR (donor) Plasmid for Inserting PuroR-2A-GFP-AID into the 5' end of the Mouse Srcap Gene
    Depositor
    Mark Groudine
    Insert
    Srcap (Srcap Mouse, Mustard Weed)
    Use
    Donor plasmid
    Tags
    GFP, AID, PuroR
    Expression
    Mutation
    Promoter
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127903-plasmid-map-sequence-id-251069

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Showing: 1461 - 1480 of 6769 results