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Plasmid#154056PurposeLevel 0 Golden Gate vector, contains domesticated NAM-B1DepositorInsertTaNAM-B1
UseSynthetic BiologyTagsExpressionBacterialMutationSynonymous mutation at base 3257, in exon 3, to r…PromoterN/AAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM54
Plasmid#154157PurposeFirefly luciferase under the control of 8 copies of the Aedes aegypti Rel2 NF-κB siteDepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoter8 copies of the Aedes aegypti Rel2 NF-κB siteAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM44
Plasmid#154153PurposeFirefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoterDepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoterDrosophila melanogaster minimal Hsp70 promoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
ELK1 A box-NLS pcDNA3.1/HisC
Plasmid#121096PurposeExpression of the A box fragment of ELK1DepositorInsertELK1 (Elk1 Mouse)
UseTags6XHis-Omni-xpressExpressionMammalianMutationPromoterAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
ELK1 B box-NLS pcDNA3.1/HisC
Plasmid#121095PurposeExpression of the B box fragment of ELK1DepositorInsertELK1 (Elk1 Mouse)
UseTags6XHis-Omni-xpressExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-Cas3
Plasmid#209427PurposePlasmid for CASCADE-Cas3 based genome engineering of streptomycetesDepositorInsertcodon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPtipA; crRNA under control of PermE*Available sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P3nmt1-GFP
Plasmid#39289DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutation-1163 to +6 of nmt1 locusPromoterAvailable sinceSept. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GFP(S65T)-His3MX6
Plasmid#41598DepositorInsertHis3
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionYeastMutationPromoterTEFAvailable sinceMay 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-2-EWSR1
Plasmid#46384DepositorInsertEWSR1 (EWSR1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-LIN-41
Plasmid#52716Purposeretroviral expression of human LIN-41 / TRIM71DepositorInsertLIN-41 (TRIM71 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_T804N_hPGK1_mScarlet-I_Donor
Plasmid#173207PurposeHDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I cassette to ATP1A1 intron 17.DepositorInsertATP1A1-T804N mScarlet-I donor
UseCRISPRTagsExpressionMammalianMutationPromoterhPGK1Available sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28 His6-mEGFP-D4
Plasmid#226372PurposeProduction of recombinant mEGFP-D4, a fluorescent probe binding cholesterolDepositorInsertD4 (pfoA Clostridium perfringens)
UseTagsHis6 and monomeric EGFPExpressionBacterialMutationPromoterT7Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_10E
Plasmid#91209PurposeDirect Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers , Plant Selection: noneDepositorInsert35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
mbYFPQS
Plasmid#80742Purposemembrane-targeted biosensor for monitoring changes in intracellular chlorideDepositorInsertYFPQS
UseTagspalmitoylation signal sequence of neuromodulinExpressionMammalianMutationH148Q (CAC to CAG) and V163S (GTG to AGC).PromoterCMVAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
Integrin beta1-tev-BHCys
Plasmid#27314DepositorInsertintegrin beta1 (ITGB1 Human)
UseTagsExpressionMammalianMutationintegrin beta1(1-708)+GG+TEV+GGKN+BHCysPromoterAvailable sinceApril 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-beta-3
Plasmid#27289DepositorInsertintegrin beta 3 (ITGB3 Human)
UseTagsHis and MycExpressionMammalianMutationn/aPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKM7
Plasmid#154164PurposemCherry expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.DepositorInsertmCherry
UseTagsExpressionInsectMutationPromoterIE1 promoter and hr5 enhancer from Autographa cal…Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
plexm-human LRRC33
Plasmid#111600PurposeExpress human LRRC33 in 393T cellsDepositorInsertLRRC33 (NRROS Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTJV1Sc-rpoB
Plasmid#166978PurposeGenerates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistanceDepositorInsertsUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23101 and VanCCAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P3nmt1
Plasmid#39280DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutation-1163 to +6 of nmt1 locusPromoterAvailable sinceAug. 14, 2012AvailabilityAcademic Institutions and Nonprofits only