We narrowed to 2,695 results for: SR

1. pCAG-FLEX-fwd[Chrimson-tdT]

   Plasmid

   #59137

   Purpose

   Expression vector for Chrimson-tdTomato in a floxed/forward-reading, Cre-dependent manner (Cre turns gene off)

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chrimson-tdTomato
   

   Use

   Cre/Lox

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGAPTrap-eGFP-IRESMygro

   Plasmid

   #82503

   Purpose

   Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene.

   Depositor

   Ed Stanley

   Article

   Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

   Insert

   eGFP-IRESMygro
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSUMO His6 SUMO PYL1 (36-211)

   Plasmid

   #177843

   Purpose

   Bacterial Expression of PYL1

   Depositor

   Karsten Melcher

   Article

   Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

   Insert

   PYR1-like 1 (PYL1 Mustard Weed)
   

   Use

   Tags

   6xHis-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1α1.1-GFP

   Plasmid

   #122100

   Purpose

   AAV-mediated expression of GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Has Service

   AAV2

   Insert

   GFP
   

   Use

   AAV

   Tags

   NA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a（1.1kb short version)

   Available since

   May 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1α1.1-FLPX-rc [Chronos-GFP]

   Plasmid

   #122102

   Purpose

   AAV-mediated expression of Chronos-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a（1.1kb short version)

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQC MCS IRES Puro

   Plasmid

   #110343

   Purpose

   γ-Retroviral transfer vector for cloning and expressing your gene of interest. IRES-driven Puromycin selection.

   Depositor

   Sandra Martha Gomes Dias

   Article

   Adamoski et al Nat Struct Mol Biol. 2023 Dec;30(12):190

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. VARP GFP-pLXIN

   Plasmid

   #62950

   Purpose

   full length human VARP with C-terminal EGFP tag cloned into pLXIN

   Depositor

   Paul Luzio

   Article

   Hesketh et al Dev Cell. 2014 Jun 9;29(5):591-606. doi:

   Insert

   VARP (ANKRD27 Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   silent mutations of amino acids 422-428 to produc…

   Promoter

   LTR

   Available since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

8. AAV-CAG-FLPX-rc [Chronos-tdTomato]

   Plasmid

   #105834

   Purpose

   AAV-mediated expression of Chronos-tdTomato under the CAG promoter in frt/reversed (Flp-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-tdTomato
   

   Use

   AAV

   Tags

   tdTomato (codon diversified version)

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCW57-mCherry-2A-BRD4 Iso AdelCTD

   Plasmid

   #137722

   Purpose

   Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform missing its C-terminal, P-TEFb interacting domain (CTD)

   Depositor

   Scott Floyd

   Article

   Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

   Insert

   BRD4 long isoform without its C-terminal, P-TEFb interacting domain (BRD4 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Truncated at amino acid 1328

   Promoter

   Tight TRE promoter

   Available since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1α-FLEX-rc[Chronos-GFP]

    Plasmid

    #62725

    Purpose

    AAV-mediated expression of Chronos-GFP under the EF1α promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA 3.1 (+) FLAG-Ago2 S387E

    Plasmid

    #60653

    Purpose

    Expresses CMV-FLAG-Ago2 (N-term tag) S387E mutant in mammalian cells

    Depositor

    Carl Novina

    Article

    Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    CMV-FLAG-Ago2 (N-term tag)

    Expression

    Mammalian

    Mutation

    S387E mutant

    Promoter

    CMV

    Available since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX 4T-1 GST-Ago2 S387A

    Plasmid

    #60656

    Purpose

    Expresses GST-Ago2 S387A mutant in mammalian cells

    Depositor

    Carl Novina

    Article

    Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    S387A

    Promoter

    tac

    Available since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA 3.1 (+) FLAG-Ago2 S387A

    Plasmid

    #60652

    Purpose

    Expresses CMV-FLAG-Ago2 (N-term tag) S387A mutant in mammalian cells

    Depositor

    Carl Novina

    Article

    Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    CMV-FLAG-Ago2 (N-term tag)

    Expression

    Mutation

    S387A mutant

    Promoter

    CMV

    Available since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGAPTrap-IRESMuro

    Plasmid

    #82336

    Purpose

    Base vector targeting genes to the GAPDH locus of human cells. Inserted genes are expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    IRESMuro
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST26 Ago2 S387A

    Plasmid

    #59905

    Purpose

    Expresses 6xHis-Ago2 (N-termi tag) S387A mutant in mammalian cells

    Depositor

    Carl Novina

    Article

    Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    6xHis (N-termi tag)

    Expression

    Mammalian

    Mutation

    S387A, phospho-null alanine

    Promoter

    CMV

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. DSPQ-HTT-TALEN2 BSD

    Plasmid

    #92246

    Purpose

    TALEN targeting downstream of HTT gene (ELD) with Blasticidin selection, forms obligate heterodimer with DSPQ-HTT-TALEN1 ZEO. TALEN: NN, HD, NN, NN, HD, NG, NN, NI, NN, NN, HD, NI, NN, HD, NI, NN

    Depositor

    Mahmoud Pouladi

    Article

    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

    Insert

    TALEN
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. DSPQ-HTT-TALEN1 ZEO

    Plasmid

    #92245

    Purpose

    TALEN targeting downstream of HTT gene (KKR) with Blasticidin selection, forms obligate heterodimer with DSPQ-HTT-TALEN2 BSD. TALEN: HD, NI, NN, HD, NG, NG, HD, HD, NG, HD, NI, NN, HD, HD, NN, HD

    Depositor

    Mahmoud Pouladi

    Article

    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

    Insert

    TALEN
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-EF1α1.1-Chronos-GFP

    Plasmid

    #122098

    Purpose

    AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α (1.1 kb short version)

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGAPTrap-mtagtdTom_IRESMuro

    Plasmid

    #82355

    Purpose

    Vector targeting the mTagtandem tomato gene to the GAPDH locus of human cells. It is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    mtagTandemTomato-IRESMuro
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQC MCS IRES G418

    Plasmid

    #110344

    Purpose

    γ-Retroviral transfer vector for cloning and expressing your gene of interest. IRES-driven Geneticin selection.

    Depositor

    Sandra Martha Gomes Dias

    Article

    Adamoski et al Nat Struct Mol Biol. 2023 Dec;30(12):190

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge