We narrowed to 2,521 results for: crispr system
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Plasmid#117323PurposeCRISPR-Cas9 for miR-124-3, 5'DepositorAvailable SinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only
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PB-U6insert-EF1puro
Plasmid#104537PurposeA piggybac vector with puromycin resistance and a U6 promoter that allows cloning of guide RNAsDepositorTypeEmpty backboneUseCRISPR, Mouse Targeting, and Synthetic Biology ; …ExpressionMammalianPromoterU6Available SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKHH030
Plasmid#89358PurposehU6 sgRNA cloning vector for CDKO systemDepositorInsertGFP, Puromycin resistance
UseCRISPR and LentiviralExpressionMammalianAvailable SinceApril 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDB4282
Plasmid#98701PurposeA plasmid containing the 3' portion of the ura4 marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-ura4 systemDepositorInsertgRNA PCR template
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB4283
Plasmid#98702PurposeA plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX systemDepositorInsertgRNA PCR template
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS
Plasmid#60904PurposeThe plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64DepositorInsertscFv-GCN4
UseLentiviralExpressionMammalianAvailable SinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-Cas9-recX
Plasmid#158715PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recXDepositorInsertcas9
UseCRISPRExpressionBacterialPromoterTetOAvailable SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-Cas12a-recX
Plasmid#158714PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses FnCpf1, NHEJ machinery of M. marinum and recXDepositorInsertrecX
UseCRISPRExpressionBacterialPromoterPmycAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-Cas12a
Plasmid#158713PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis, expresses FnCpf1, NHEJ machinery of M. marinumDepositorInsertthe MmNHEJ machinery (MMAR_4573, MMAR_4574, and MMAR_4575)
UseCRISPRExpressionBacterialAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS424-ysg(G:H)
Plasmid#138257PurposeExpression of GFP-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequence to be used in yeast dual reporter systemDepositorInsertGFP targeting sgRNAs G and H
UseCRISPRExpressionYeastPromoterSNR52Available SinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ202
Plasmid#86198PurposeMultisite Gateway T-DNA entry plasmid (attR1 & attR2); AtUBQ10 promoter and Hygromycin resistance markerDepositorTypeEmpty backboneUseGateway compatible attr1-attr2 destination vectorExpressionPlantPromoterArabidopsis ubiquitin 10 (AtUbi10)Available SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLQ9757: CMV-BPNLS_TadA-linkerA-TadA*(8e)-Linker B-SV40 NLS-dCasMINI-V4-linkerC-c-Myc_NLS-3x FLAG-polyA
Plasmid#176270PurposeExpression of TadA-TadA* (8e)-dCasMINI-V4 in mammalian cellsDepositorInsertTadA-TadA*(8e)-dCasMINI-V4
ExpressionMammalianPromoterCMVAvailable SinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
th-P2A-EGFP donor
Plasmid#65562PurposeIntron targeting-mediated EGFP knockin at the zebrafish th locus.DepositorAvailable SinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDIV301
Plasmid#204969PurposeAMA1 plasmid with Aspergillus optimized Mad7 and ble (bleomycin) resistance markerDepositorInsertsMad7
ble (bleomycin resistance marker)
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialPromoterAspergillus nidulans tef1 promoter and Aspergillu…Available SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro-CAG-GCaMP6s
Plasmid#120896PurposeDonor plasmid targeting human AAVS1 safe harbor locus to generate GCaMP6s gene knockin. Contains two homologous arms flanking T2A-Puro resistance gene and CAG-driven GCaMP6s gene.DepositorInsertGCaMP6s
ExpressionMammalianPromoterCAGAvailable SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiCD19_H1_Gal4VP64
Plasmid#169918PurposeExpression of a synNotch receptor containing antiCD19, a human Notch1 core, and Gal4VP64.DepositorInsertantiCD19-Notch1-GL4VP64
UseLentiviralExpressionMammalianPromoterPGKAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
th-P2A-Gal4 donor
Plasmid#65563PurposeIntron targeting-mediated Gal4 knockin at the zebrafish th locus.DepositorAvailable SinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSH-EFIRES-P-AtAFB2
Plasmid#129715PurposeAAVS1 safe harbor targeting vector expressing AtAFB2DepositorInsertAFB2 (AFB2 Mustard Weed)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsnoExpressionMammalianMutationcodon optimizedPromoterEF1aAvailable SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPV-EF1a-EGxxFP-iP-A
Plasmid#204624PurposeCloning vector for SSA vector (EGxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestionDepositorTypeEmpty backboneExpressionMammalianAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPV-EF1a-mRxxFP1(AfeI)-iP-A
Plasmid#204625PurposeCloning vector for SSA vector (mRxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestionDepositorTypeEmpty backboneExpressionMammalianAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only