We narrowed to 30,996 results for: uros

1. pLenti Puro HA-Ubiquitin-K63

   Plasmid

   #236291

   Purpose

   Lentiviral vector expressing HA-tagged ubiquitin K63 mutant

   Depositor

   Melina Fan, Addgene Research Program

   Insert

   Ubiquitin K63 (UBB Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Single ubiquitin coding sequence containing K63 m…

   Promoter

   CMV

   Available since

   April 10, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. qTAG-C-mStayGold-Puro-CANX

   Plasmid

   #227281

   Purpose

   Donor template for mStayGold-2A-Puro insertion into the C-terminus of the CANX locus. For cilia visualization. To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   CANX Homology Arms flanking a mStayGold-2A-Puro Cassette (CANX Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBABE puro SDF-1 alpha

   Plasmid

   #12270

   Depositor

   Bob Weinberg

   Article

   Orimo et al Cell. 2005 May 6. 121(3):335-48.

   Insert

   SDF-1 alpha (CXCL12 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-TetOne-Puro-MCL1

   Plasmid

   #202663

   Purpose

   Tet-ON 3rd generation expression vector with inducible expression of MCL1 and a puromycin selection cassette

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   MCL1 apoptosis regulator, BCL2 family member (MCL1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPBT/EFS-Cas9-PuroTk

   Plasmid

   #170542

   Purpose

   Expresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinase

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

   Insert

   PuroTK
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV x EF1a_EKAREN5(TA)-ires-puro

   Plasmid

   #167823

   Purpose

   control sensor for EKAREN5

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN5(TA)
   

   Use

   Lentiviral

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W, T420A

   Promoter

   EF1a

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSicoR-Ef1a-mCh-Puro

   Plasmid

   #31845

   Purpose

   Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression. Includes puromycin selection.

   Depositor

   Bruce Conklin

   Article

   Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

   Type

   Empty backbone

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for shRNA; Ef1alpha for mCherry-puromycin

   Available since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAVS1 hPGK-PuroR-pA donor

   Plasmid

   #22072

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   PGK-Puro (PGK1 Human)
   

   Use

   Zfn donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. OCT4-2A-eGFP-PGK-Puro

   Plasmid

   #31938

   Purpose

   Human OCT4 genomic targeting donor

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

   Insert

   OCT4 (POU5F1 Human)
   

   Use

   Targeting donor

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCVpuro-hAIM2-DsRed monomer

    Plasmid

    #51609

    Purpose

    Express DsRed monomer-tagged hAIM2 in mammalian cells

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    AIM2 (AIM2 Human)
    

    Use

    Retroviral

    Tags

    DsRed monomer

    Expression

    Mammalian

    Mutation

    Promoter

    MESV

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro

    Plasmid

    #28289

    Purpose

    3rd generation lentiviral plasmid; This plasmid is the backbone for the DECIPHER libraries (currently DISCONTINUED)

    Depositor

    Alex Chenchik, Gus Frangou

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-mCherry-CAAX PuroR

    Plasmid

    #166228

    Purpose

    Lentiviral plasmid with Puromycin selection for expression of mCherry with a C-terminal CAAX polybasic sequence from KRras targeting mCherry to the plasma membrane

    Depositor

    Carlos Carmona-Fontaine

    Article

    Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

    Insert

    mCherry-CAAX
    

    Use

    Lentiviral

    Tags

    CAAX

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1-puro-irf3-shrna1

    Plasmid

    #127648

    Purpose

    Knock-down of human IRF3

    Depositor

    Nicolas Manel

    Article

    Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

    Insert

    IRF3 shRNA (IRF3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-HA-UBE3BΔHECT-IRES-Puro

    Plasmid

    #107347

    Purpose

    Expresses HA tagged UBE3B deletion of HECT domain in mammalian cells.

    Depositor

    Robert Sobol

    Article

    Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

    Insert

    UBE3BΔHECT (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deletion HECT domain (757-end)

    Promoter

    CMV

    Available since

    June 26, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pMXs-puro GFP-DFCP1

    Plasmid

    #38269

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Autophagy. 2010 Aug;6(6):764-76.

    Insert

    Zinc finger FYVE domain-containing protein 1 (Zfyve1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. FASTHDR-Cterm-HaloTag-Puromycin

    Plasmid

    #167208

    Purpose

    Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with Puromycin

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH-puro-CMV-CC3AI

    Plasmid

    #78909

    Purpose

    Indicator of caspase-3-like protease activity

    Depositor

    Binghui Li

    Article

    Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

    Insert

    CC3AI
    

    Use

    Lentiviral

    Tags

    HA and Myc

    Expression

    Mutation

    S320A, deletion of AA1-200

    Promoter

    CMV

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pMKO.1 puro GFP shRNA

    Plasmid

    #10675

    Purpose

    Negative control vector for pMKO.1 puro (Addgene plasmid #8452); Contains shRNA against GFP.

    Depositor

    William Hahn

    Article

    Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

    Insert

    GFP shRNA
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMXs-puro GFP-p62 dC

    Plasmid

    #38282

    Depositor

    Noboru Mizushima

    Article

    Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

    Insert

    Sequestosome-1 (Sqstm1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Truncate mutant 1-265 amino acids residues

    Promoter

    Available since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. qTAG-N-Puro-moxGFP-LMNB1

    Plasmid

    #207773

    Purpose

    Donor template for Puro-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LMNB1 Homology Arms flanking a Puro-moxGFP Cassette (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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