163,354 results

1. phCMV-229E-D15

   Plasmid

   #188908

   Purpose

   express truncated 229E spike for pseudovirus production

   Depositor

   David Nemazee

   Insert

   229E spike (S 229E)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   c-terminal 15aa deletion

   Promoter

   CMV

   Available since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiCRISPRi(v2)-Blast

   Plasmid

   #170068

   Purpose

   Expresses dCas9 repressor KRAB-dCas9-MeCP2 and blasticidin resistance

   Depositor

   Neville Sanjana

   Article

   Morris et al Science. 2023 May 19;380(6646):eadh7699.

   Insert

   KRAB-dCas9-MeCP2
   

   Use

   CRISPR and Lentiviral

   Tags

   2A and 2xHA tag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. 4xM67 pTATA TK-Luc

   Plasmid

   #8688

   Depositor

   Jim Darnell

   Article

   Besser et al Mol Cell Biol 1999 Feb;19(2):1401-9.

   Insert

   4 repeated M67 sites
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBroad-T7RNAP-chlorR

   Plasmid

   #199992

   Purpose

   T7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) chlorR

   Depositor

   Farren Isaacs

   Article

   Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

   Insert

   T7RNAP Circuit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   copFnull

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRE-mCherry-p2A-eNpHR3.0-TS

   Plasmid

   #158703

   Purpose

   Reporter construct

   Depositor

   Alice Ting

   Article

   Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

   Insert

   mCherry-p2A-eNpHR3.0-TS
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEBG-Pak1 83-149

   Plasmid

   #12214

   Depositor

   Jonathan Chernoff

   Article

   Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

   Insert

   Pak1 (PAK1 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Dominant negative Pak1: autoinhibitory domain, aa…

   Promoter

   Available since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-Mm.cargo(Cas9)

   Plasmid

   #174865

   Purpose

   Expresses mouse Peg10 cargoRNA encoding SpCas9

   Depositor

   Feng Zhang

   Article

   Segel et al Science. 2021 Aug 20;373(6557):882-889.

   Insert

   SpCas9 flanked by MmPeg10 UTRs
   

   Use

   Tags

   HA-SV40 NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. 2X_pX458_pSpCas9(BB)-2A-GFP

   Plasmid

   #172221

   Purpose

   Cas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.

   Depositor

   Alexander Meissner

   Article

   Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKanCMV_mRuby3

   Plasmid

   #172854

   Purpose

   mRuby3 expressed under the CMV promotor

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   mRuby3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-CRY2clust

    Plasmid

    #105624

    Purpose

    Expresses improved CRY2PHR homo-oligomerization

    Depositor

    Won Do Heo

    Article

    Park et al Nat Commun. 2017 Jun 23;8(1):30. doi: 10

    Insert

    mCherry-CRY2PHR-9aa (CRY2 Mustard Weed, Human)
    

    Use

    Tags

    9 amino acids

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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