We narrowed to 2,171 results for: D10

1. pLenti_dCas9-2xAM_hIRF-1

   Plasmid

   #92221

   Purpose

   Lentiviral plasmid expressing dCas9-2xAM and gRNA for human IRF-1 promoter

   Depositor

   Hodaka Fujii

   Article

   Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

   Inserts

   Sp-dCas9-2xAM tag
   gRNA targeting human IRF-1 promoter
   

   Use

   CRISPR and Lentiviral

   Tags

   2xAM tag

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   CBh and U6

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. CABE-T2.6

   Plasmid

   #193263

   Purpose

   Expression of CABE-T2.6 base editor in mammalian cells, CMV promoter

   Depositor

   Nicole Gaudelli

   Article

   Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   CABE-T2.6
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A (Cas9), TadA mutations within

   Promoter

   CMV

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-C-intein-C-spC9-H840A-N863A-2xNLS-hGH

   Plasmid

   #112211

   Purpose

   Targeted DNA methylation

   Depositor

   Xingxu Huang

   Article

   Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

   Insert

   C-terminus of dCas9
   

   Use

   AAV

   Tags

   Expression

   Mutation

   D10A

   Promoter

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. CBE-T1.6

   Plasmid

   #193275

   Purpose

   Expression of CBE-T1.6 base editor in mammalian cells, CMV promoter

   Depositor

   Nicole Gaudelli

   Article

   Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   CBE-T1.6
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A (Cas9), TadA mutations within

   Promoter

   CMV

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDIRECT_21D

   Plasmid

   #91131

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A, H840A

   Promoter

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. PET11a-Af2sir2

   Plasmid

   #8383

   Depositor

   Cynthia Wolberger

   Article

   Avalos et al Mol Cell 2002 Sep;10(3):523-35.

   Insert

   Sir2-Af2 full length (SIRT2, Sir2.2, SIRT1 Archaeoglobus Fulgidus)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   PET11a-Af2Sir2D103N

   Promoter

   Available since

   Dec. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. 360 pCR kdrb

   Plasmid

   #13067

   Depositor

   Nathan Lawson

   Article

   Covassin et al Proc Natl Acad Sci U S A. 2006 Apr 25. 1

   Insert

   kdrb (kdr Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 18, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pMEH60 Lamp2b MS2 HA

   Plasmid

   #168209

   Purpose

   Mammalian expression of lysosome-associated membrane protein 2b fused to the MS2 dimer and C-terminal HA tag

   Depositor

   Joshua Leonard

   Article

   Hung et al J Extracell Vesicles. 2016 May 13;5:3102

   Insert

   Lamp2b (LAMP2 Human)
   

   Use

   Tags

   HA and MS2 dimer

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJT45_GalL_nCDA1-BE3

   Plasmid

   #145038

   Purpose

   Expresses nCDA1-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

   Insert

   nCDA1-BE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A)

   Promoter

   GalL

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMyc-CMV1-huTLR4mut-C2141A

    Plasmid

    #53527

    Purpose

    expression of mutant TLR4 in mammalian cells

    Depositor

    Linda Yu

    Article

    Kuo et al Cell Death Differ. 2015 Jan 30. doi: 10.

    Insert

    TLR4 C2141A (TLR4 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    C2141A

    Promoter

    CMV

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJT79_GalL_nCDA1Δ192-BE3

    Plasmid

    #145045

    Purpose

    Expresses nCDA1Δ192-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    nCDA1Δ192-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    pmCDA1(1-192AA); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-myc-Sema4DdeltaC

    Plasmid

    #51602

    Purpose

    expresses Sema4D lacking C terminus

    Depositor

    Suzanne Paradis

    Article

    Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

    Insert

    Sema4DdeltaC (Sema4d Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Last 69 amino acids of Sema4D C-terminus are dele…

    Promoter

    CMV

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pMyc-CMV1-huTLR4mut-C1196T

    Plasmid

    #53526

    Purpose

    expression of mutant TLR4 in mammalian cells

    Depositor

    Linda Yu

    Article

    Kuo et al Cell Death Differ. 2015 Jan 30. doi: 10.

    Insert

    TLR4 C1196T (TLR4 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    C1196T

    Promoter

    CMV

    Available since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMyc-CMV1-huTLR4mut-A896G

    Plasmid

    #53525

    Purpose

    expression of mutant TLR4 in mammalian cells

    Depositor

    Linda Yu

    Article

    Kuo et al Cell Death Differ. 2015 Jan 30. doi: 10.

    Insert

    TLR4 A896G (TLR4 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    A896G

    Promoter

    CMV

    Available since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. Unc5b.z-AP-His

    Plasmid

    #72053

    Purpose

    Expresses the extracellular region of the Unc5B, isoform z protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5b.z (Unc5b Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-Hook3 I136A, I139A, M142A

    Plasmid

    #74617

    Purpose

    Expresses a triple mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, I136A, I139A, M142A (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    D102A, E108A

    Promoter

    T7

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. KIF5A-mVenus-iLID

    Plasmid

    #240422

    Purpose

    +end oriented kinesin for optogenetic pulling experiments

    Depositor

    Anna Akhmanova

    Article

    Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    iLID and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBABE FLAG HA p100 (Ser707/711Ala)

    Plasmid

    #236469

    Purpose

    stable expression of p100 using virus infection

    Depositor

    Michele Pagano

    Article

    Busino et al Nat Cell Biol. 2012 Mar 4;14(4):375-85.

    Insert

    p100 (NFKB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ser707/711Ala

    Promoter

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. cBEST4

    Plasmid

    #234660

    Purpose

    Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion

    Depositor

    Mingzi M. Zhang

    Article

    Lin et al Appl Environ Microbiol. 2025 Mar 10:e019

    Inserts

    spCas9 cytosine base editor
    Golden Gate compatible sgRNA insertion cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P3 and tcp830

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHEE901-A3A

    Plasmid

    #229044

    Purpose

    nCas9 mediated-base editing with NGG PAM recognition motif in Arabidopsis. A3A-nCas9-UGI includes D10A in Cas9.

    Depositor

    Huawei Zhang

    Article

    Wang et al Genome Biol. 2024 Aug 9;25(1):215. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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