We narrowed to 11,926 results for: pcDNA

1. TRAPa-3xFLAG-pCDNA

   Plasmid

   #201963

   Purpose

   Mammalian expression of Translocon-associated protein subunit alpha

   Depositor

   Ville Paavilainen

   Article

   Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

   Insert

   SSR1 (SSR1 Human)
   

   Use

   Tags

   3xFLAG-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pY006 (pcDNA3.1-hBpCpf1)

   Plasmid

   #69978

   Purpose

   Expresses humanized BpCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hBpCpf1
   

   Use

   CRISPR

   Tags

   NLS & 3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3-FLAG-MKRN1_H307E

   Plasmid

   #78756

   Purpose

   To overexpress MKRN1_H307E in Mammalian Cell

   Depositor

   Jaewhan Song

   Article

   Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

   Insert

   MKRN1 (MKRN1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   H307E

   Promoter

   CMV

   Available since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA 3.1(+) eGFP p6

   Plasmid

   #78582

   Purpose

   Expresses GFP p6 in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Gurer et al J Virol. 2005 Jan;79(2):910-7.

   Insert

   eGFP p6
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HIV-1 HXB2 p6 with conservative mutations (G78A, …

   Promoter

   Available since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1(+)_Paragranulin-no linker

   Plasmid

   #176917

   Purpose

   express Paragranulin without linker 1 in mammalian cells

   Depositor

   Thomas Kukar

   Article

   Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

   Insert

   Paragranulin (GRN Human)
   

   Use

   Tags

   Twin-Strep and FLAG tags encoded after Signal Pep…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.3-hALDOA D34S

   Plasmid

   #210810

   Purpose

   To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

   Depositor

   Sheng-cai Lin

   Article

   Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

   Insert

   ALDOA (ALDOA Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   residues 34 of ALDOA mutated to serine

   Promoter

   CMV

   Available since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3 10 mut B-Myb

   Plasmid

   #25966

   Depositor

   Rob Lewis

   Article

   Johnson et al J Biol Chem. 1999 Dec 17. 274(51):36741-

   Insert

   B-Myb (Mybl2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed threonines 267, 408, 497, 519, 522 and 52…

   Promoter

   Available since

   Sept. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1: TfR1-CoilE

   Plasmid

   #181988

   Purpose

   CoilE genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with CoilR labeled with a reporter

   Depositor

   Kimberly Beatty

   Article

   Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

   Insert

   TfR1-CoilE
   

   Use

   Tags

   CoilE

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. EPHA3_1-972 pcDNA3.1

   Plasmid

   #102739

   Purpose

   Expression of EPHA3 in mammalian cells

   Depositor

   Jin Chen

   Article

   Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

   Insert

   EPHA3 (EPHA3 Human)
   

   Use

   Tags

   myc-6xhis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. XE213 RGS11-pcDNA3.1

    Plasmid

    #16675

    Depositor

    Randall Moon

    Insert

    RGS11 (RGS11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1-DTXK-lumitoxin

    Plasmid

    #51691

    Purpose

    Expression vector for DTXK-lumitoxin

    Depositor

    Edward Boyden

    Article

    Schmidt et al Nat Commun. 2014 Jan 10;5:3019. doi: 10.

    Insert

    DTXK-lumitoxin
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3 Connexin43-APEX

    Plasmid

    #44439

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

    Insert

    Connexin43 fused to pea APEX
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    K14D, W41F, E112K mutations on APEX

    Promoter

    CMV

    Available since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDNA3 Flag Jnk2b1

    Plasmid

    #13756

    Depositor

    Roger Davis

    Article

    Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

    Insert

    Jnk2b1 (MAPK9 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA4/TO-BcRF1-2xStrep

    Plasmid

    #138436

    Purpose

    Expresses full-length BcRF1 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    BcRF1 (BcRF1 EBV (HHV-4))
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pY013 (pcDNA3.1-hEeCpf1)

    Plasmid

    #69985

    Purpose

    Expresses humanized EeCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hEeCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA-LmoCas6-p300

    Plasmid

    #126486

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas6-p300
    

    Use

    CRISPR

    Tags

    Flag and Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-PKCZeta KR HA Tagged

    Plasmid

    #24608

    Depositor

    Jeff Wrana

    Article

    Wang et al Science. 2003 Dec 5. 302(5651):1775-9.

    Insert

    PKCZeta (Prkcz Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K281R mutation in the ATP binding domain causing …

    Promoter

    Available since

    June 7, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

18. mCHOP10(dLZ).pCDNA1

    Plasmid

    #21900

    Depositor

    David Ron

    Article

    Ron et al Genes Dev. 1992 Mar;6(3):439-53. doi: 10

    Insert

    CHOP10 (Ddit3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The coding region of the murine CHOP-10 cDNA was …

    Promoter

    Available since

    Oct. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

19. CHAF1B_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176969

    Purpose

    Mammalian expression vector encoding CHAF1B and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    CHAF1B (CHAF1B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5FRT/TO-YOD1-Strep-HA

    Plasmid

    #113483

    Purpose

    Expression of human YOD1 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    YOD1 (YOD1 Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA6-N-3XFLAG-Fzd6

    Plasmid

    #123588

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    frizzled class receptor 6 (Fzd6 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1-P2X2-CFP

    Plasmid

    #22401

    Depositor

    Henry Lester

    Article

    Khakh et al J Neurosci. 2005 Jul 20. 25(29):6911-20.

    Insert

    ATP-gated P2X channel 2 (P2rx2 Rat)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

23. pCDNA3 Flag MKK7b2

    Plasmid

    #14623

    Depositor

    Roger Davis

    Article

    Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

    Insert

    MKK7b2 (Map2k7 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

24. pcDNA-Cx43-YA/VD-GFP

    Plasmid

    #60703

    Purpose

    mammalian expression of rat Cx43 YA/VD mutant fused to GFP

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    YA/VD (Y286A/V289D)

    Promoter

    CMV

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

25. pCDNA6-LRRC38-V5-His

    Plasmid

    #127820

    Purpose

    Expression of human LRRC38 in mammalian cells

    Depositor

    Jiusheng Yan

    Article

    Li et al J Gen Physiol. 2015 Jun;145(6):543-54. d

    Insert

    LRRC38 (LRRC38 Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA5-LAP-KNL1-M3

    Plasmid

    #115896

    Purpose

    Used for expression of siRNA-resistant KNL1-M3 (modified codons 258 and 259) from the FRT site of FlpIn cells upon addition of doxycyclin.

    Depositor

    Geert Kops

    Article

    Vleugel et al J Cell Biol. 2013 Dec 23;203(6):943-55.

    Insert

    KNL1-M3 (KNL1 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Fragment of KNL1 containing three KNL1 repeat seq…

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA5-FRT-TO-Nsp5

    Plasmid

    #157685

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp5 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. Y921F EphA2 pcDNA3

    Plasmid

    #102736

    Purpose

    Expression of EphA2 in mammalian cells

    Depositor

    Jin Chen

    Article

    Fang et al J Biol Chem. 2008 Jun 6;283(23):16017-26

    Insert

    EphA2 (Epha2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y921F (TAC > TTC)

    Promoter

    CMV

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3-FLAG_FBXL7 (R310H)

    Plasmid

    #171849

    Purpose

    pcDNA3-FLAG_FBXL7 (R310H)

    Depositor

    Michele Pagano

    Article

    Moro et al Nat Cell Biol. 2020 Sep;22(9):1130-1142.

    Insert

    F-box and leucine rich repeat protein 7 (FBXL7 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed Arginine 310 to Histidine

    Promoter

    Available since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA5/FRT/TO DNAJA1

    Plasmid

    #19464

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJA1 (DNAJA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D244G

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pY019 (pcDNA3.1-hPmCpf1)

    Plasmid

    #69991

    Purpose

    Expresses humanized PmCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hPmCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pcDNA3.1-nAChr-Alpha6

    Plasmid

    #48812

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr-alpha6 (Chrna6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Kohinoor-Paxillin/pcDNA3

    Plasmid

    #67775

    Purpose

    Paxilin-targetted Kohinoor for mammalian cell expression

    Depositor

    Takeharu Nagai

    Article

    Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

    Insert

    Kohinoor
    

    Use

    Tags

    Paxillin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

34. pY009 (pcDNA3.1-hSsCpf1)

    Plasmid

    #69981

    Purpose

    Expresses humanized SsCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hSsCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

35. ciCas9(L22)_pcDNA5

    Plasmid

    #100551

    Purpose

    Expresses ciCas9(L22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

    Insert

    ciCas9(L22)
    

    Use

    CRISPR; Frt, flp-in

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA-Cx43-Myc

    Plasmid

    #60693

    Purpose

    mammalian expression of Myc-tagged rat Cx43

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

37. pcDNA3-Lyn-GR-AKAR3

    Plasmid

    #173016

    Purpose

    Plasma membrane-targeted variant of improved green-red FRET-based PKA activity reporter.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    Lyn-GR-AKAR3
    

    Use

    Tags

    EGFP, N-terminal targeting sequence from Lyn kina…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCDNA3.1-hMcl-1(T163A)

    Plasmid

    #25376

    Depositor

    Roger Davis

    Article

    Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

    Insert

    hMCL1 (MCL1 Human)
    

    Use

    Tags

    V5 and His tag

    Expression

    Mammalian

    Mutation

    changed threonine 163 to alanine

    Promoter

    Available since

    Aug. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

39. pCDNA4.TO-K4.1-2xCSTREP

    Plasmid

    #136152

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K4.1

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    K4.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCDNA4.TO-K10-2xCSTREP

    Plasmid

    #136163

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K10

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    K10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge