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Showing: 3701 - 3750 of 16058 results

  7. FLAG-MBP1-E322TAG-E309C-W340A-CAAX.pcDNA3-k

    Plasmid
    #127409
    Purpose
    Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site
    Depositor
    Sharona Gordon
    Article
    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1
    Insert
    Maltose Binding Protein
    Use
    Tags
    CAAX and FLAG
    Expression
    Mammalian
    Mutation
    Amino acid mutations: E322 to Amber codon, E309C …
    Promoter
    CMV
    Available since
    July 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    127409-plasmid-map-sequence-id-248721

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  8. FLAG-MBP1-K295TAG-W340A-S233H-T237C-CAAX.pcDNA3-k

    Plasmid
    #127403
    Purpose
    Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site
    Depositor
    Sharona Gordon
    Article
    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1
    Insert
    Maltose Binding Protein
    Use
    Tags
    CAAX and FLAG
    Expression
    Mammalian
    Mutation
    Amino acid mutations: K295 to Amber codon, W340A,…
    Promoter
    CMV
    Available since
    July 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    127403-plasmid-map-sequence-id-248720

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  9. FLAG-MBP1-E322TAG-K305H-E309H-W340A-CAAX.pcDNA3-k

    Plasmid
    #127407
    Purpose
    Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site
    Depositor
    Sharona Gordon
    Article
    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1
    Insert
    Maltose Binding Protein
    Use
    Tags
    CAAX and FLAG
    Expression
    Mammalian
    Mutation
    Amino acid mutations: E322 to Amber codon, K305H,…
    Promoter
    CMV
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127407-plasmid-map-sequence-id-247970

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  12. FLAG-MBP1-K295TAG-W340A-S233H-T237H-CAAX.pcDNA3-k

    Plasmid
    #127401
    Purpose
    Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site
    Depositor
    Sharona Gordon
    Article
    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1
    Insert
    Maltose Binding Protein
    Use
    Tags
    CAAX and FLAG
    Expression
    Mammalian
    Mutation
    Amino acid mutations: K295 to Amber codon, W340A,…
    Promoter
    CMV
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127401-plasmid-map-sequence-id-247963

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  29. pfMDH_K381A

    Plasmid
    #67732
    Purpose
    Expresses K381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed lysine 381 to alanine
    Promoter
    T7
    Available since
    Sept. 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  30. pfMDH_N300D

    Plasmid
    #67728
    Purpose
    Expresses N300D mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed asparagine 300 to aspartate
    Promoter
    T7
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  31. pfMDH_R373A

    Plasmid
    #67731
    Purpose
    Expresses R373A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed arginine 373 to alanine
    Promoter
    T7
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  33. pfMDH_N300S

    Plasmid
    #67729
    Purpose
    Expresses N300S mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed asparagine 300 to serine
    Promoter
    T7
    Available since
    Sept. 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  39. pAS-7 (mm12-21)

    Plasmid
    #40842
    Purpose
    Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
    Depositor
    Phillip Zamore
    Article
    Ameres et al Science. 2010 Jun 18;328(5985):1534-9.
    Insert
    let-7–mm12-21
    Use
    Mirna reporter
    Tags
    EGFP
    Expression
    Insect
    Mutation
    Promoter
    Actin5C
    Available since
    Sept. 4, 2013
    Availability
    Academic Institutions and Nonprofits only

  40. pAS-6 (mm17-21)

    Plasmid
    #40841
    Purpose
    Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
    Depositor
    Phillip Zamore
    Article
    Ameres et al Science. 2010 Jun 18;328(5985):1534-9.
    Insert
    let-7–mm17-21
    Use
    Mirna reporter
    Tags
    EGFP
    Expression
    Insect
    Mutation
    Promoter
    Actin5C
    Available since
    Sept. 4, 2013
    Availability
    Academic Institutions and Nonprofits only

  41. pAS-5 (mm18-21)

    Plasmid
    #40840
    Purpose
    Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
    Depositor
    Phillip Zamore
    Article
    Ameres et al Science. 2010 Jun 18;328(5985):1534-9.
    Insert
    let-7–mm18-21
    Use
    Mirna reporter
    Tags
    EGFP
    Expression
    Insect
    Mutation
    Promoter
    Actin5C
    Available since
    Sept. 4, 2013
    Availability
    Academic Institutions and Nonprofits only

  42. pAS-4 (mm19-21)

    Plasmid
    #40839
    Purpose
    Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
    Depositor
    Phillip Zamore
    Article
    Ameres et al Science. 2010 Jun 18;328(5985):1534-9.
    Insert
    let-7–mm19-21
    Use
    Mirna reporter
    Tags
    EGFP
    Expression
    Insect
    Mutation
    Promoter
    Actin5C
    Available since
    Sept. 4, 2013
    Availability
    Academic Institutions and Nonprofits only

  43. pAS-3 (mm20-21)

    Plasmid
    #40838
    Purpose
    Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
    Depositor
    Phillip Zamore
    Article
    Ameres et al Science. 2010 Jun 18;328(5985):1534-9.
    Insert
    let-7–mm20-21
    Use
    Mirna reporter
    Tags
    EGFP
    Expression
    Insect
    Mutation
    Promoter
    Actin5C
    Available since
    Sept. 4, 2013
    Availability
    Academic Institutions and Nonprofits only

  46. pAS14 (mm16-21)

    Plasmid
    #40849
    Purpose
    Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
    Depositor
    Phillip Zamore
    Article
    Ameres et al Science. 2010 Jun 18;328(5985):1534-9.
    Insert
    let-7–mm16-21
    Use
    Mirna reporter
    Tags
    EGFP
    Expression
    Insect
    Mutation
    Promoter
    Actin5C
    Available since
    Sept. 4, 2013
    Availability
    Academic Institutions and Nonprofits only

  47. pAS-12 (mm1-8)

    Plasmid
    #40847
    Purpose
    Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
    Depositor
    Phillip Zamore
    Article
    Ameres et al Science. 2010 Jun 18;328(5985):1534-9.
    Insert
    let-7–mm1-8
    Use
    Mirna reporter
    Tags
    EGFP
    Expression
    Insect
    Mutation
    Promoter
    Actin5C
    Available since
    Sept. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 3701 - 3750 of 16058 results