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We narrowed to 2,537 results for: PORA

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Showing: 1481 - 1500 of 2537 results

  7. SSH-pSUMO-α-COPI-WD40

    Plasmid
    #208341
    Purpose
    Bacterial expression plasmid for Schizosaccharomyces pombe α-COPI-WD40
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    N-terminal WD40 domain of yeast α-COPI (1-327) with substitutions (Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys and Phe197Lys)
    Tags
    His-tag, SUMO-tag, and Strep-tag
    Expression
    Bacterial
    Mutation
    Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys and Ph…
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    208341-plasmid-map-sequence-id-436201

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  10. pCRI011-pYFAC-pyroA-PgpdA-4xcrRNAarrayelcA-TtrpC

    Plasmid
    #140203
    Purpose
    Fungal vector to express an LbCas12a crRNA array targeted to Pelca, to be contransformed with pCRI009 containing PelcA-mCherry in a dLbCas12a-VPR strain as proof-of-concept of CRISPRa.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Insert
    crRNA array elcA
    Use
    CRISPR and Synthetic Biology
    Promoter
    PgpdA
    Available Since
    Sept. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    140203-plasmid-map-sequence-id-310119

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  11. FB013

    Plasmid
    #119710
    Purpose
    TU of the HSVtk negative marker for F2dU domesticated into pUPD2 with GGAG/TACT barcodes (reverse orientation). Used for gene KO with dual selec. According to FungalBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Negative selection marker HSVtk (Pgpda:HSVtk:Ttub)
    Use
    Synthetic Biology; Domestication of dna parts for…
    Available Since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119710-plasmid-map-sequence-id-237145

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  12. pmCherry-3xNLS-OptoJNKi-dsm

    Plasmid
    #89743
    Purpose
    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11
    Depositor
    Michael Courtney
    Article
    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10
    Insert
    NLS-OptoJNKi dark-state mutant
    Use
    Fluorescent protein
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    C450A mutation of AsLov2Jalpha
    Promoter
    CMV
    Available Since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    89743-plasmid-map-sequence-id-192503

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  13. pmCherry-3xNLS-OptoJNKi-lsm

    Plasmid
    #89742
    Purpose
    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11
    Depositor
    Michael Courtney
    Article
    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10
    Insert
    NLS-OptoJNKi lit-state mutant
    Use
    Fluorescent protein
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    I539E mutation of AsLov2Jalpha
    Promoter
    CMV
    Available Since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    89742-plasmid-map-sequence-id-192502

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  20. pSA075 Cas9-BFP-HBB_IVS2

    Plasmid
    #249136
    Purpose
    Overexpression of SpCas9-BFP with HBB IVS2 intron in 3' UTR and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    Cas9-TagBFP-HBB_IVS2 (HBB Human, S. pyogenes Cas9, synthetic)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249136-plasmid-map-sequence-id-496444

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Showing: 1481 - 1500 of 2537 results