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Plasmid#182335PurposeExpresses human FOXA1 and miRFP670 via T2A linker under control of a CMV promoter.DepositorInsertForkhead Box A1 (FOXA1 Human)
UseTagsT2A-miRFP670ExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV-FOXC1-T2A-miRFP670
Plasmid#182336PurposeExpresses human FOXC1 and miRFP670 via T2A linker under control of a CMV promoter.DepositorInsertForkhead Box C1 (FOXC1 Human)
UseTagsInserted T2A-miRFP670 at 3' terminal of MCS.ExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
psLIB TRE H2B iRFP720
Plasmid#229968Purposeencodes a H2B-iRFP720 fusion contruct under the control of a dox inducible promoterDepositorInsertH2B iRFP720
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yemiRFP670-Kan
Plasmid#224088PurposeYeast tagging vector to create a gene fusion with yeast optimized emiRFP670 with Kan selectionDepositorInsertyemiRFP670
UseTagsyemiRFP670ExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yemiRFP670-SpHis5
Plasmid#224098PurposeYeast tagging vector to create a gene fusion with yeast optimized emiRFP670 with SpHis5 selectionDepositorInsertyemiRFP670
UseTagsyemiRFP670ExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
fabp10a:FLAG-SpiCee-mRFP1; cryaa:mCherry
Plasmid#230062PurposeExpression of genetically encoded calcium chelator in zebrafish hepatocytes.DepositorInsertSpiCee
UseZebrafish expressionTagsFLAG and mRFP1ExpressionMutationPromoterfabp10aAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLViN-iRFP670-α-cateninA+ΔβH
Plasmid#229707PurposeLentiviral expression of iRFP670-alpha-cateninA+delta-beta-H in mammalian cellsDepositorInsertCTNNA1 (alpha-catenin) Human (CTNNA1 Human)
UseLentiviralTagsiRFP670ExpressionMammalianMutationamino acids 670-673, RAIM--> GSGSPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR-TRE-miRFP703_PGK-Puro
Plasmid#216667PurposeExpresses tTa-inducible miRFP703, with puromycin resistanceDepositorInsertsmiRFP703
PuroR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-miRFP670nano3-H3C2
Plasmid#227335PurposeDonor template for miRFP670nano3 insertion into the C-terminus of the H3C2 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 (Addgene #207780)DepositorInsertH3C2 Homology Arms flanking a miRFP670nano3 Tag (H3C2 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-miRFP670nano3-H2BC11
Plasmid#227331PurposeDonor template for miRFP670nano3 insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)DepositorInsertH2BC11 Homology Arms flanking a miRFP670nano3 Tag (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-9YS-miRFP670-TRF1
Plasmid#227381PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric FUS IDR, 9YS (TERF1 Human)
UseLentiviralTagsExpressionMutation9YSPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-27YS-miRFP670-TRF1
Plasmid#227383PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric FUS IDR, 27YS (TERF1 Human)
UseLentiviralTagsExpressionMutation27YSPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-15YS-miRFP670-TRF1
Plasmid#227382PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric FUS IDR, 15YS (TERF1 Human)
UseLentiviralTagsExpressionMutation15YSPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-5YS-miRFP670-TRF1
Plasmid#227380PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric FUS IDR, 5YS (TERF1 Human)
UseLentiviralTagsExpressionMutation5YSPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-3YS-miRFP670-TRF1
Plasmid#227379PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric FUS IDR, 3YS (TERF1 Human)
UseLentiviralTagsExpressionMutation3YSPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-a(+)-6xHis-miRFP(-5)-HO1
Plasmid#219424PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorInsertsmiRFP682
HO1
UseTagsMGHHHHHGGExpressionBacterialMutationContains Ribosomal Binding Site for expression of…PromoterT7Available sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME_Golgi-BFP_P2A_H2A_iRFP (JDW 1007)
Plasmid#224490PurposeGateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-iRFP (BFP golgi reporter and infrared RFP nuclear reporter)DepositorInsertGolgi-mTagBFP-P2A-H2A-iRFP
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME_Golgi-BFP_P2A_H2A_tdiRFP (JDW 1079)
Plasmid#224491PurposeGateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-tdiRFP (BFP golgi reporter and tandem infrared RFP nuclear reporter)DepositorInsertGolgi-mTagBFP-P2A-H2A-tdiRFP
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDRF1 ymiRFP680-T2A-mTurquoise2
Plasmid#219840Purposeequimolar expression of ymiRFP680 and mTurquoise2 in budding yeastDepositorInsertymiRFP680-T2A-mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 27, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDRF1 yemiRFP670-T2A-mTurquoise2
Plasmid#219841Purposeequimolar expression of yemiRFP670 and mTurquoise2 in budding yeastDepositorInsertyemiRFP670-T2A-mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 27, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits