We narrowed to 5,621 results for: pcas

1. pCAG-mcherry-WIPI2dR108E-cs-TEV-STREP

   Plasmid

   #171842

   Purpose

   Mammalian expression of human WIPI2d with N-terminal mCherry and C-terminal Strep

   Depositor

   James Hurley

   Article

   Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

   Insert

   WIPI2d (WIPI2 Human)
   

   Use

   Tags

   Strep and mCherry

   Expression

   Mammalian

   Mutation

   R108E

   Promoter

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-mcherry-WIPI2dR125E-cs-TEV-STREP

   Plasmid

   #171843

   Purpose

   Mammalian expression of human WIPI2d R108E with N-terminal mCherry and C-terminal Strep

   Depositor

   James Hurley

   Article

   Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

   Insert

   WIPI2d (WIPI2 Human)
   

   Use

   Tags

   Strep and mCherry

   Expression

   Mammalian

   Mutation

   R125E

   Promoter

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-mcherry-WIPI2dK128E-cs-TEV-STREP

   Plasmid

   #171844

   Purpose

   Mammalian expression of human WIPI2d K128E with N-terminal mCherry and C-terminal Strep

   Depositor

   James Hurley

   Article

   Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

   Insert

   WIPI2d (WIPI2 Human)
   

   Use

   Tags

   Strep and mCherry

   Expression

   Mammalian

   Mutation

   K128E

   Promoter

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-OSF-PP-squirrel-monkey-CHMP3

   Plasmid

   #154182

   Purpose

   expresses squirrel monkey CHMP3 in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

   Insert

   CHMP3
   

   Use

   Tags

   FLAG, Strep-Tag II, and preScission site

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-OSF-PP-squirrel-monkey-retroCHMP3

   Plasmid

   #154184

   Purpose

   expresses squirrel monkey retroCHMP3 in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

   Insert

   retroCHMP3
   

   Use

   Tags

   FLAG, Strep-Tag II, and preScission site

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCaggs-NLS-PAmCherry1-GSS-EGFP

   Plasmid

   #158000

   Purpose

   Expression of nucleus localized PAmCherry1 and EGFP fusion protein

   Depositor

   Francois St-Pierre

   Article

   Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

   Insert

   NLS-PAmCherry1-GSS-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Actin promoter with CMV enhancer

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAAA)

   Plasmid

   #137860

   Purpose

   Monomeric TagBFP2 and iCre-ERT2 recombinase (PAAA N-terminus)

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-iCreERT2
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAAAA)

   Plasmid

   #137861

   Purpose

   Monomeric TagBFP2 and iCre-ERT2 recombinase (PAAAA N-terminus)

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-iCreERT2
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAGSA)

   Plasmid

   #137862

   Purpose

   Monomeric TagBFP2 and iCre-ERT2 recombinase (PAGSA N-terminus)

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-iCreERT2
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAAGSA)

    Plasmid

    #137863

    Purpose

    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAAGSA N-terminus)

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCreERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAGGS-mTagBFP2-T2A-iCreERT2 (PVV)

    Plasmid

    #128172

    Purpose

    Monomeric TagBFP2 and iCre-ERT2 recombinase (PVV N-terminus)

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCreERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAGGS-mTagBFP2-T2A-iCreERT2 (PVGSA)

    Plasmid

    #128173

    Purpose

    Monomeric TagBFP2 and iCre-ERT2 recombinase (PVGSA N-terminus)

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCreERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MEI-S332-GFP genomic in pCaSpeR

    Plasmid

    #113174

    Purpose

    In P element vector for transposon insertion into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Article

    Kerrebrock et al Cell. 1995 Oct 20;83(2):247-56.

    Insert

    mei-S332 (Sgo)
    

    Use

    P insertion vector

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX459V2.0-eSpCas9(1.1)+K810A+K855A

    Plasmid

    #108299

    Purpose

    pX459 V2.0 (Plasmid #62988) with the K810A, K848A, K855A, K1003A, and R1060A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBW759_pCAG-PV2-lox2272-GFP-BGHpA

    Plasmid

    #87575

    Purpose

    PV2 for 2-input, 4-output decoder circuit and 3-input, 2-output Full Adder

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    EGFP
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBW807_pCAG-PV4-loxP-mCherry-BGHpA

    Plasmid

    #87583

    Purpose

    PV4 for 3-input, 2-output Full Adder

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    mCherry
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBW770_pCAG-PV4-loxP-mRuby2-BGHpA

    Plasmid

    #87577

    Purpose

    PV4 for 2-input, 4-output decoder circuit

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    mRuby2
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBW707_pCAG-PV3-FRT-loxP-mCherry

    Plasmid

    #87571

    Purpose

    PV3 for 2-input, 2-output circuit library

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    mCherry
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBW705_pCAG-PV1-FRT-lox2272-STOP

    Plasmid

    #87569

    Purpose

    PV1 for 2-input, 2-output circuit library

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    Neomycin Resistance Gene
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCASCADE-gltA1-gltA2-udhA-zwf

    Plasmid

    #87152

    Purpose

    gltA1-gltA2-udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction

    Depositor

    Michael Lynch

    Insert

    gltA1-gltA2-udhA-zwf guide array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCASCADE-gltA1-gltA2-udhA-GapAP1

    Plasmid

    #87151

    Purpose

    gltA1-gltA2-udhA-GapAP1 targeting guide RNA E. coli , Low Phosphate Induction

    Depositor

    Michael Lynch

    Insert

    gltA1-gltA2-udhA-GapAP1 guide array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCASCADE-fabI-gltA1-gltA2-gapAP1

    Plasmid

    #87147

    Purpose

    fabI-gltA1-gltA2-gapAP1 targeting guide RNA E. coli , Low Phosphate Induction

    Depositor

    Michael Lynch

    Insert

    fabI-gltA1-gltA2-gapAP1 guide array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

    Plasmid

    #139999

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

    Promoter

    CAG

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p23-NES-PspCas13b-msfGFP-NES-Flag

    Plasmid

    #165071

    Purpose

    overexpression of PspCas13b in human cells

    Depositor

    Ling-Ling Chen

    Article

    Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

    Insert

    PspCas13b
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Ef1a-pspCas13b-NES-3xFLAG-T2A-BFP

    Plasmid

    #173029

    Purpose

    For expression of cytoplasmic pspCas13b protein tagged with 3xHA-T2A-BFP for gene silencing in mamallian cells.

    Depositor

    Mohamed Fareh

    Article

    Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

    Insert

    pspCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG, BFP, HIV NES, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)

    Plasmid

    #185910

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpCas9-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A

    Promoter

    CMV and T7

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-pCAG eGFP T2A SP HALO HAPLN1

    Plasmid

    #228200

    Purpose

    AAV vector expressing GFP and HaloTag-HAPLN1 under constitutive promoter

    Depositor

    Sungjin Park

    Article

    Sterin et al J Neurosci. 2024 Sep 9:e0666242024. doi:

    Insert

    HAPLN1 (Hapln1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    constitutive CAG promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAGGS puro-GFP-BamHI-IRES-mCherry

    Plasmid

    #232247

    Purpose

    AcGFP1-BamHI-IRES-mCherry empty backbone

    Depositor

    Francisco Bustos

    Article

    Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pT3TS-spCas9 ABE-Umax-ex2-rest1

    Plasmid

    #222143

    Purpose

    Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Expression

    Mutation

    Promoter

    T3

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pT3TS-spCas9 ABE-Umax-ex1-rest1

    Plasmid

    #222140

    Purpose

    Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Expression

    Mutation

    Promoter

    T3

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge