We narrowed to 11,246 results for: VARS

1. pETM33_DCUN1D1_PONY

   Plasmid

   #178473

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   DCUN1D1_PONY (DCUN1D1 Human, Synthetic)
   

   Tags

   His-GST

   Expression

   Bacterial

   Promoter

   T7/LacO

   Available Since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM33_KLC1_TPR

   Plasmid

   #178477

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   KLC1_TPR (KLC1 Human, Synthetic)
   

   Tags

   His-GST

   Expression

   Bacterial

   Promoter

   T7/LacO

   Available Since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPICZ-aC-VTS-US

   Plasmid

   #160425

   Purpose

   Expression of c-luciferase from sp. Vargula tsujii (USA)

   Depositor

   Todd Oakley

   Article

   Hensley et al Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.1

   Insert

   luciferase
   

   Use

   Luciferase

   Tags

   6x Histidine tag, Yeast alpha secretion factor, c…

   Expression

   Yeast

   Mutation

   See depositor comments below

   Promoter

   AOX

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. GRB2-SHN

   Plasmid

   #139625

   Purpose

   StrepIII-HA-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

   Insert

   GRB2 (GRB2 Human)
   

   Tags

   StrepIII-HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDD2034

   Plasmid

   #101377

   Purpose

   Name: pBluescript-Δ611-843REV. Blue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), 611-843 REV.

   Depositor

   Dirk Dittmer

   Article

   Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

   Insert

   Blue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), 611-843 REV
   

   Use

   Unspecified

   Mutation

   Blue Heron Biotechnology Inc. bidirectional mutan…

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. p3R112C/T180CHFm

   Plasmid

   #85091

   Purpose

   Expresses PCNA3 variant (R112C/T180C) fused to heme-FMN domain of P450 BM3 variant (A74G) in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   The R112C/T180C variant of PCNA3 fused to Heme-FMN domain of P450 BM3
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   R112C/T180C in PCNA3, A74G in heme-FMN domain of …

   Promoter

   T7 promoter

   Available Since

   Dec. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBABE-puro-THAP11-rescue-3xFLAG

   Plasmid

   #36998

   Depositor

   Debu Chakravarti

   Article

   Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

   Insert

   THAP11 (THAP11 Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Mutations were generated at codons 273-275 conver…

   Available Since

   May 21, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

   Plasmid

   #139998

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

   Promoter

   CAG

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLEX-uORF-HA-birA*-N-Ras(wildtype)-IRES-Puro

   Plasmid

   #120563

   Purpose

   Expresses HA-birA*-N-Ras wildtype fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro

    Plasmid

    #120564

    Purpose

    Expresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Q61K

    Promoter

    CMV

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti_ABE8e-SpRY-P2A-BFP_HygroR

    Plasmid

    #213009

    Purpose

    A lentiviral vector used to create stable cell lines expressing the ABE8e-SpRY base editor and BFP

    Depositor

    Richard Sherwood

    Article

    Ryu et al Nat Genet. 2024 Apr 24. doi: 10.1038/s41

    Insert

    ABE8e-SpRY-D10A
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A nickase variant of SpRY

    Promoter

    Ef-1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVITRO1-dV-IgG1/κ

    Plasmid

    #52213

    Purpose

    Human immunoglobulin gamma 1 antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    Human immunoglobulin gamma 1 constant region
    Human immunoglobulin kappa constant region
    

    Expression

    Mammalian

    Mutation

    Deleted Variable region

    Promoter

    Mouse Elongation Factor 1 Alpha and Rat Elongatio…

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVITRO1-dV-IgE/κ

    Plasmid

    #61878

    Purpose

    Human immunoglobulin epsilon antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    Human immunoglobulin Epsilon constant region
    Human immunoglobulin kappa constant region
    

    Expression

    Mammalian

    Mutation

    Deleted Variable region

    Promoter

    mEF1 Prom and rEF1 Prom

    Available Since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVITRO1-dV-IgE/λ

    Plasmid

    #61879

    Purpose

    Human immunoglobulin epsilon antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    Human immunoglobulin Epsilon constant region
    Human immunoglobulin lambda constant region
    

    Expression

    Mammalian

    Mutation

    Deleted Variable region

    Promoter

    mEF1 Prom and rEF1 Prom

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. p3XFLAG-CMV-14-WDR5

    Plasmid

    #59974

    Purpose

    Mammalian Expression of WDR5-FLAG

    Depositor

    Debu Chakravarti

    Article

    Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

    Insert

    WDR5 (WDR5 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Oct. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVITRO1-dV-IgG1/λ

    Plasmid

    #52214

    Purpose

    Human immunoglobulin gamma 1 antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    Human immunoglobulin gamma 1 constant region
    Human immunoglobulin lambda constant region
    

    Expression

    Mammalian

    Mutation

    Deleted Variable region

    Promoter

    Mouse Elongation Factor 1 Alpha and Rat Elongatio…

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. B-Sniper SpCas9

    Plasmid

    #207361

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-Sniper SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    F539S, M763I, K890N, amino acids 1005-1013 replac…

    Promoter

    CBh

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. MAC-FURIN

    Plasmid

    #158386

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    furin, paired basic amino acid cleaving enzyme (FURIN Human)
    

    Tags

    MAC-tag

    Expression

    Mammalian

    Available Since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pcDNA3_erGAP3

    Plasmid

    #78118

    Purpose

    Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum

    Depositor

    Javier García-Sancho, Teresa Alonso

    Article

    Navas-Navarro et al Cell Chem Biol. 2016 Jun 1. pii: S2451-9

    Insert

    erGAP3
    

    Tags

    KDEL and calreticulin signal peptide

    Expression

    Mammalian

    Mutation

    S175G, D180Y, I167V and L15Q in GFP moiety and D…

    Promoter

    CMV

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV5-hBMPR-1A CA

    Plasmid

    #49527

    Purpose

    expresses constitutively active human BMP receptor 1A

    Depositor

    Peter ten Dijke, Lee Niswander

    Article

    Varley et al Dev Biol. 1998 Apr 1;196(1):107-18.

    Insert

    BMPR-1A CA (BMPR1A Human)
    

    Expression

    Mammalian

    Mutation

    constitutively active mutation (Q233D); also P2A …

    Promoter

    CMV

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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