Addgene

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pMD2.G-VSV-G-mut

Plasmid

#196508

Purpose

VSV-G mutant (K47Q, R354A)

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

VSV-G

Use

Tags

NA

Expression

Mammalian

Mutation

K47Q, R354A

Promoter

CMV

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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SpyCatcher003-mi3

Plasmid

#159995

Purpose

Expresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccination

Depositor

Mark Howarth

Article

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

SpyCatcher003-mi3

Use

Tags

C-Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBad-sfGFP

Plasmid

#85482

Purpose

Arabinose inducible E. coli codon optimized superfolder GFP with C-terminal His6 tag

Depositor

Ryan Mehl

Article

Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

Insert

superfolder GFP

Use

Tags

6x His

Expression

Bacterial

Mutation

Promoter

Arabinose

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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peYFP-C1-mTOR

Plasmid

#73384

Purpose

Expresses YFP tagged mTOR

Depositor

Jie Chen, Taekjip Ha

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

mTOR (MTOR Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pEF.myc.ER-E2-Crimson

Plasmid

#38770

Purpose

In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)

Depositor

Benjamin Glick

Article

Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

Insert

E2-Crimson

Use

Tags

ER retention signal (KDEL), ER signal sequence (M…

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA-NHERF1

Plasmid

#31650

Depositor

Edward Weinman

Article

Weinman et al Biochemistry. 2003 Nov 4. 42(43):12662-8

Insert

solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)

Use

Tags

His & S tag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only

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mTurquoise2

Plasmid

#198196

Purpose

Spectral correction: Expression of mTurquoise2

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

A206K

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBad-EBFP2

Plasmid

#14891

Purpose

Bacterial expression of EBFP2 blue fluorescent protein

Depositor

Robert Campbell

Article

Ai et al Biochemistry. 2007 Apr 20. ():.

Has Service

Cloning Grade DNA

Insert

EBFP2

Use

Tags

His

Expression

Bacterial

Mutation

The fluorescent protein was inserted between XhoI…

Promoter

Available since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

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pEBFP2-Nuc

Plasmid

#14893

Purpose

Mammalian expression of EBFP2 blue fluorescent protein with NLS

Depositor

Robert Campbell

Article

Ai et al Biochemistry. 2007 Apr 20. ():.

Insert

pEBFP2-Nuc

Use

Tags

NLS

Expression

Mammalian

Mutation

Replace EYFP of the vector pEYFP-Nuc from Clontec…

Promoter

Available since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

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FLAG-3C-sGFP11_pcDNA3.1(-)

Plasmid

#182932

Purpose

For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP11 fragment

Depositor

William Weis

Article

Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

Type

Empty backbone

Use

Tags

3C-cleavable split GFP 11, FLAG tag, and HA signa…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-3C-sGFP1-10_pcDNA3.1(-)

Plasmid

#182931

Purpose

For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragment

Depositor

William Weis

Article

Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

Type

Empty backbone

Use

Tags

3C-cleavable split GFP 1-10, FLAG tag, and HA sig…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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RGG-BcLOV-mCh

Plasmid

#208276

Purpose

Expresses RGG-BcLOV fusion for optogenetic membrane recruitment. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

RGG-BcLOV-mCh

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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Fus-BcLOV-mCh

Plasmid

#208277

Purpose

Expresses Fus-BcLOV fusion for optogenetic membrane recruitment. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

Fus-BcLOV-mCh

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pBad-CA

Plasmid

#105665

Purpose

expresses thermostable variant of human carbonic anhydrase II with C-terminal 6x his tag

Depositor

Ryan Mehl

Article

Mehl et al 2020

Insert

human carbonic anhydrase II thermostable variant (CA2 Human)

Use

Tags

6x his tag

Expression

Bacterial

Mutation

Promoter

pBad/arabinose

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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MXS_CreERT2

Plasmid

#62427

Purpose

MXS_chaining vector with CreERT2

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Tamoxifen inducible Cre recombinase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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plvx-AD8

Plasmid

#191558

Purpose

Inducible expression of HIV-1 Env

Depositor

Alon Herschhorn, Joseph Sodroski

Article

Herschhorn et al PLoS One. 2011;6(11):e26731. doi: 10.137

Insert

HIV-1 AD8 env

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Hs.KRAS4b G13D

Plasmid

#83133

Purpose

Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G13D mutation

Depositor

Dominic Esposito

Insert

KRAS (KRAS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

G13D

Promoter

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pJBL7004

Plasmid

#136943

Purpose

Malachite green aptamer, T7 promoter

Depositor

Julius Lucks

Article

Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

Insert

malachite green aptamer (MGA)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pT7

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221-GNMT

Plasmid

#166177

Purpose

Gateway entry vector for N. furzeri GNMT

Depositor

Daniel Jarosz

Article

Yiwen R. Chen et al bioRxiv

Insert

GNMT

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAG244 iFAST

Plasmid

#130809

Purpose

Expresses iFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Biochemistry. 2018 Oct 2;57(39):5648-565

Insert

iFAST

Use

Tags

His-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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