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pDONR223 SARS-CoV2 TEV-NSP7

Plasmid

#154406

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP7 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector, with insert of n…

Mutation

Many synonymous changes due to codon optimization

Available since

July 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pKCAG-mCherry-OptoJNKi

Plasmid

#89738

Purpose

Expression of light-regulated JNK inhibitor, wild-type photosensor, inhibitor JIP11

Depositor

Michael Courtney

Publication

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

OptoJNKi

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Promoter

CAG

Available since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAcBac3-pAAFRS-8xYtR-EGFP-3TAA- 40TGA-152TAG-TEV-3xUAA

Plasmid

#228788

Purpose

EcTyr aaRS/tRNA system for pAAF incorporation with EGFP containing three stop codons for triple suppression

Depositor

Abhishek Chatterjee

Publication

Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

Inserts

EcTyrRS
EcYtR
EGFP***
TEV protease

Use

Baculoviral

Expression

Mammalian

Mutation

TAG suppressor

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pmCherry-3xNLS-OptoJNKi-dsm

Plasmid

#89743

Purpose

Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11

Depositor

Michael Courtney

Publication

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

NLS-OptoJNKi dark-state mutant

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Mutation

C450A mutation of AsLov2Jalpha

Promoter

CMV

Available since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-3xNLS-OptoJNKi-lsm

Plasmid

#89742

Purpose

Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11

Depositor

Michael Courtney

Publication

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

NLS-OptoJNKi lit-state mutant

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Mutation

I539E mutation of AsLov2Jalpha

Promoter

CMV

Available since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100)-IRES-puromycin

Plasmid

#223965

Purpose

Dual fluorescent reporter for histone H2B and for Cdt1

Depositor

Hee Won Yang

Publication

Zhang et al Nat Commun. 2023 Nov 29;14(1):7847. doi:

Insert

H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100) (H2B, CDT1 Human)

Use

Lentiviral

Tags

iRFP670 and mCerulean

Expression

Mammalian

Promoter

EF-1a

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3.1-N-Myc_DW164AA USP7

Plasmid

#131252

Purpose

Mammalian expression of N-terminally Myc-tagged DW164AA USP7

Depositor

Roger Woodgate

Publication

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Mutation

Contains a point mutation that converts USP7 aspa…

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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PP-ASL

Plasmid

#40749

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

Trypanosoma Brucei Adenylosuccinate Lyase

Tags

6xHis TEV

Expression

Bacterial

Available since

Oct. 18, 2012

Availability

Industry, Academic Institutions, and Nonprofits
pLEW100v5-BSD-VSG-228

Plasmid

#245116

Purpose

Inserts VSG-228 coding sequence in T. brucei rDNA spacer

Depositor

Monica Mugnier

Publication

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228

Use

Other

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pHD309-PUR-VSG-228

Plasmid

#245105

Purpose

Inserts VSG-228 coding sequence in T. brucei tubulin array

Depositor

Monica Mugnier

Publication

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228

Use

Other

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228

Plasmid

#245106

Purpose

Inserts VSG-228 coding sequence in T. brucei rDNA spacer

Depositor

Monica Mugnier

Publication

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228

Use

Other

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228.500bp

Plasmid

#245110

Purpose

Inserts 500 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694

Depositor

Monica Mugnier

Publication

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228:500

Use

Other

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228.400bp

Plasmid

#245111

Purpose

Inserts 400 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694

Depositor

Monica Mugnier

Publication

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228:400

Use

Other

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228.300bp

Plasmid

#245112

Purpose

Inserts 300 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694

Depositor

Monica Mugnier

Publication

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228:300

Use

Other

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228.200bp

Plasmid

#245113

Purpose

Inserts 200 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694

Depositor

Monica Mugnier

Publication

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228:200

Use

Other

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228.100bp

Plasmid

#245114

Purpose

Inserts 100 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694

Depositor

Monica Mugnier

Publication

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228:100

Use

Other

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228.100bp_offset

Plasmid

#245115

Purpose

Inserts 100 bp of VSG-228 coding sequence in T. brucei rDNA spacer overlapping AnTat1.1 position 694, centered around common VSG-228 insertion

Depositor

Monica Mugnier

Publication

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228:100_offset

Use

Other

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pAIP4_2H7W

Plasmid

#234220

Purpose

Heterologous protein expression of chagasin in Escherichia coli

Depositor

Christopher Bahl

Publication

Cárcamo Noriega et al Protein Sci. 2026 Sep;35(9):e70746. doi:

Insert

2H7W (Tc00.1047053506801.80 Trypanosoma cruzi)

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLEW100v5-PUR-VSG-228.noUTRS

Plasmid

#245109

Purpose

Inserts VSG-228 coding sequence without UTRs in T. brucei rDNA spacer

Depositor

Monica Mugnier

Publication

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228

Use

Other

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-177-PUR-VSG-228

Plasmid

#245107

Purpose

Inserts VSG-228 coding sequence in T. brucei minichromosomes using the 177bp repeats

Depositor

Monica Mugnier

Publication

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228

Use

Other

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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