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We narrowed to 14,343 results for: nol

Showing: 3041 - 3080 of 14343 results

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pCMV_AncBE4max_3xHA

Plasmid

#112097

Purpose

C:G-to-T:A base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

AncBE4max_3xHA

Use

CRISPR

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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RSVpGL3 (RSV promoter-luciferase in pGL3-basic)

Plasmid

#40343

Depositor

Alexander Dent

Article

Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

Insert

RSV promoter

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

RSV

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pQE30-His-NDK

Plasmid

#124136

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Nucleotide diphosphate kinase (ndk Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-Vimentin-7

Plasmid

#55156

Purpose

Localization: Intermediate Filaments, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

Insert

Vimentin (VIM Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
p201G Cas9

Plasmid

#59178

Purpose

Cas9 driven by double 35S, GFP for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
sGFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

2x35S

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pBT120a17

Plasmid

#138510

Purpose

P1

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

gIII-xLuxAB

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pMK393 (mAID-mCherry-BSD)

Plasmid

#121194

Purpose

C-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

mAID-mCherry-BSD

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pQE30-His-IF1

Plasmid

#124123

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Initiation factor 1 (infA Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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TRE-ChrimsonR-mCherry

Plasmid

#92207

Purpose

Reporter construct

Depositor

Alice Ting

Article

Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

ChimsonR-mCherry

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

June 16, 2017

Availability

Academic Institutions and Nonprofits only

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pQE60-EFG-His

Plasmid

#124126

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Elongation factor G (fusA Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE30-His-IF3

Plasmid

#124125

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Initiation factor 3 (infC Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCI-neo-cOVA

Plasmid

#25097

Depositor

Maria Castro

Article

Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

Insert

cytoplasmic ovalbumin

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pQE30-His-RF3

Plasmid

#124131

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Release factor 3 (prfC Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pQE60-EFTs-His

Plasmid

#124128

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Elongation factor Ts (tsf Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET15b-His-RF2

Plasmid

#124130

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Release factor 2 (prfB Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pQE60-RRF-His

Plasmid

#124132

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Ribosome recycling factor (frr Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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MLM3613

Plasmid

#42251

Purpose

Expresses Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 promoters

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

Streptococcus pyogenes Cas9

Use

CRISPR; Zebrafish expression

Tags

Expression

Mutation

Promoter

CMV

Available since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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DL033:cFos::eGFP-iPAK4

Plasmid

#177883

Purpose

eGFP-iPAK4 fusion with cFos promoter, cloned into a lentivirus backbone

Depositor

Adam Cohen

Article

Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

iPAK4

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

cFos

Available since

July 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBT114

Plasmid

#138523

Purpose

phage cloning

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

phage genes

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ265_OoA3GX2

Plasmid

#225147

Purpose

Gateway entry clone (attL1 & attR5) OoA3GX2-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Contiliani et al Plant Biotechnol J. 2025 Feb 14. doi: 10

Insert

OoA3GX2-zCas9(D10A)-UGI

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pYL322d1/N-eGFP

Plasmid

#183151

Purpose

Plant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eGFP.

Depositor

Qinlong Zhu, Yao-Guang Liu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET-Neq2X7

Plasmid

#182366

Purpose

Expresses Neq2X7 DNA polymerase

Depositor

Morten Norholm

Article

Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

Insert

Neq2X7 DNA polymerase

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBT280

Plasmid

#122610

Purpose

express evoFERNY-BE4max in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoFERNY-BE4max

Use

Tags

Expression

Mammalian

Mutation

H102P D104N

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX - T7pro - EBFP

Plasmid

#138615

Purpose

Express EBFP with an upstream T7 promoter in a lentiviral backbone

Depositor

Fei Chen

Article

Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

EBFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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mEmerald-Keratin-17

Plasmid

#54134

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

Insert

Keratin (KRT18 Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pT2ST

Plasmid

#59384

Purpose

Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a trimethoprim resistance gene.

Depositor

Shelley Copley

Article

Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

Insert

TT-ISceI-dfrA

Use

Template

Tags

Expression

Mutation

Promoter

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBZS-M.CviPI

Plasmid

#198355

Purpose

Express M.CviPI in bacterial cells.

Depositor

Takashi Ito

Article

Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

Insert

M.CviPI (M.CviPI )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBT29

Plasmid

#138521

Purpose

phage cloning

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

phage genes

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBT100.164

Plasmid

#138522

Purpose

phage cloning

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(7.10)-inteinN

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19/N-Vc

Plasmid

#183157

Purpose

Bimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vc.

Depositor

Qinlong Zhu, Yao-Guang Liu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
BFP dest clone

Plasmid

#71825

Purpose

Lentiviral introduction of BFP reporter (Point mutation H66 in mEGFP results in BFP expression)

Depositor

Jacob Corn

Article

Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pYL322d1/N-Vn

Plasmid

#183153

Purpose

Bimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vn.

Depositor

Qinlong Zhu, Yao-Guang Liu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pORF2

Plasmid

#206026

Purpose

An expression vector for mouse LINE1 ORF2

Depositor

Masamichi Kamihira

Article

Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

Insert

ORF2 derived from mouse LINE1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JD553

Plasmid

#160392

Purpose

Wheat GRF4-GIF1 chimera in pDONR-zeo

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Insert

wheat GRF4-GIF1 chimera

Use

Entry clon

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-miniGFP2

Plasmid

#188897

Purpose

Express 6xHis-Tag miniGFP2 in E.coli

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

miniGFP2

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD441_WT_hi

Plasmid

#128857

Purpose

High copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 TaGu N-1F

Plasmid

#203762

Purpose

For expression of N-flag tagged TaGu R2 protein in mammalian cells

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

R2 retroelement protein

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 PM-Ras-LOCKR-S

Plasmid

#216498

Purpose

FRET-based reporter for Ras activity, plasma membrane localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

PM-Ras-LOCKR-S

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET30-T7-His-sfGFP

Plasmid

#180755

Purpose

pET30 vector missing lacO with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Article

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Use

Tags

strep tag

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G03 TFEC

Plasmid

#67060

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFEC (TFEC Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

Showing: 3041 - 3080 of 14343 results

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