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Showing: 3801 - 3850 of 18941 results

  5. RS35075_pMQ30_KO_Kan

    Plasmid
    #185390
    Purpose
    Non-replicating vector used to create markerless deletion of RR42_RS35075 in C. basilensis 4G11
    Depositor
    Adam Arkin
    Article
    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1
    Inserts
    RR42_RS35075 upstream flanking homology region
    RR42_RS35075 downstream flanking homology region
    nptII kanamycin resistance marker
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    185390-plasmid-map-sequence-id-371206

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  6. CRISPseq-BFP-backbone

    Plasmid
    #85707
    Purpose
    Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with a BFP fluorescent marker. Does NOT include Cas9.
    Depositor
    Ido Amit
    Article
    Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85707-plasmid-map-sequence-id-170482

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  8. pCPP5233

    Plasmid
    #128715
    Purpose
    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone
    Depositor
    Alan Collmer
    Article
    Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi
    Insert
    shcM-hopM1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    128715-plasmid-map-sequence-id-252574

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  11. JI401: Ad5 E1A/Hexon qRT-PCR standard

    Plasmid
    #131753
    Purpose
    Plasmid standard containing regions of the Ad5 E1A and Ad5 Hexon genes to create a qRT-PCR standard curve for detection of replication competent adenovirus (RCA).
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Inserts
    Ad5 E1A gene fragment
    Ad5 Hexon gene fragment
    Use
    Standard for qrt-pcr
    Tags
    Expression
    Mutation
    Promoter
    N/A
    Available since
    Oct. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    131753-plasmid-map-sequence-id-314394

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  13. pMS_375

    Plasmid
    #182132
    Purpose
    expresses retron RNA, ec.86 Reverse Transcriptase, CspRecT SSAP, and mutL* to create a specific rifampicin resistance mutation while inhibiting MMR
    Depositor
    George Church
    Article
    Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118
    Inserts
    retron RNA
    ec.86 RT
    CspRecT
    Use
    Tags
    Expression
    Mutation
    incorporated rifampicin-resistance-conferring don…
    Promoter
    Available since
    Sept. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    182132-plasmid-map-sequence-id-355786

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  15. pCPP3373

    Plasmid
    #128718
    Purpose
    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone
    Depositor
    Alan Collmer
    Article
    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(
    Insert
    hopQ1-1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Synonymous point mutation in the wobble position…
    Promoter
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    128718-plasmid-map-sequence-id-252569

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  16. pCRISPReporter-mCherry

    Plasmid
    #65008
    Purpose
    E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter.
    Depositor
    Mattheos Koffas
    Article
    Cress et al ACS Synth Biol. 2015 Mar 30.
    Insert
    mCherry
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    User-defined
    Available since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    65008-plasmid-map-sequence-id-109895

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  18. CRISPseq-mCherry-backbone

    Plasmid
    #85708
    Purpose
    Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with an mCherry fluorescent marker. Does NOT include Cas9.
    Depositor
    Ido Amit
    Article
    Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85708-plasmid-map-sequence-id-170481

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  20. Direct-seq_lentiGuide-Puro-Loop2-8A8G

    Plasmid
    #157983
    Purpose
    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.
    Depositor
    Lijia Ma
    Article
    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:
    Insert
    S. pyogenes sgRNA cassette (Loop2-8A8G)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    May 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    157983-plasmid-map-sequence-id-332422

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  22. Direct-seq_lentiGuide-Puro-Tail-8A8G

    Plasmid
    #157981
    Purpose
    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.
    Depositor
    Lijia Ma
    Article
    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:
    Insert
    S. pyogenes sgRNA cassette (Tail-8A8G)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    157981-plasmid-map-sequence-id-309444

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  23. pOCC94

    Plasmid
    #118912
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal, N-terminal MBP, cleavable with 3C protease, and C-terminal eGFP-HIS6
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HBMss-MBP-3C-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    Use
    Tags
    MBP, cleavable with 3C protease and eGFP-HIS6
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118912-plasmid-map-sequence-id-232258

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  24. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA- Pconstitutive-sgRNA(Sth3)_blaA

    Plasmid
    #133347
    Purpose
    expression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets cpaA and second one targets blaA (from Caulobacter crescentus)
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    sgRNA_cpaA and sgRNA_blaA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    constitutive
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133347-plasmid-map-sequence-id-269540

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  31. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA- Pconstitutive-sgRNA(Sth3)_cpaA

    Plasmid
    #133348
    Purpose
    expression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets blaA and second one targets cpaA (from Caulobacter crescentus)
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    sgRNA_blaA and sgRNA_cpaA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    constitutive
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133348-plasmid-map-sequence-id-267409

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  36. pLVX-TetOne-Puro-EcSTH-FLAG

    Plasmid
    #218628
    Purpose
    This plasmid contains human codon optimized sequence of EcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.
    Depositor
    Valentin Cracan
    Article
    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038
    Insert
    EcSTH
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    218628-plasmid-map-sequence-id-432188

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  37. pLVX-TetOne-Puro-mitoEcSTH-FLAG

    Plasmid
    #218629
    Purpose
    This plasmid contains human codon optimized sequence of mitoEcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.
    Depositor
    Valentin Cracan
    Article
    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038
    Insert
    mitoEcSTH
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG and MTS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    218629-plasmid-map-sequence-id-432199

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  38. pMax_ScaI_BFP

    Plasmid
    #177824
    Purpose
    Site-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).
    Depositor
    Zachary Nagel, Leona Samson
    Article
    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111
    Insert
    tagBFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    5' inserted ScaI RE cut site
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    177824-plasmid-map-sequence-id-348950

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  40. pPapi

    Plasmid
    #96921
    Purpose
    expression of S aureus SaCas9 (SauCas9) and two pol III promoters for an Sa gRNA and an Sp gRNA. Intended to be used in SpCas9-expressing cell lines for dual Cas9 "Big Papi" screens. aka pXPR207.
    Depositor
    David Root, John Doench
    Article
    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    96921-plasmid-map-sequence-id-181715

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  43. pMax_mPlum_C207G/T208C

    Plasmid
    #177831
    Purpose
    Site-specific mutagenesis of mPlum for the purposes of creating a site-specific O6-MeG lesion for the purposes of measuring MGMT activity via Host Cell Reactivation (HCR).
    Depositor
    Zachary Nagel, Leona Samson
    Article
    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111
    Insert
    mPlum_C207G/T208C
    Use
    Tags
    Expression
    Mammalian
    Mutation
    C207G + T208C
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177831-plasmid-map-sequence-id-348956

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  50. pUC57-white[coffee]

    Plasmid
    #84006
    Purpose
    Donor HR template carrying a 2,080 bp fragment of the Drosophila melanogaster white[coffee] allele and silent mutations conferring resistance to white sgRNAs-1, -2, -3, and -4
    Depositor
    Phillip Zamore
    Article
    Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.
    Insert
    A 2,080 bp fragment of the white[coffee] allele (w Fly)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    A GC-to-AA mutation that creates a G589E missense…
    Promoter
    Available since
    Nov. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    84006-plasmid-map-sequence-id-165529

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Showing: 3801 - 3850 of 18941 results