We narrowed to 12,031 results for: cel.2

1. D21_Ld_141150

   Plasmid

   #135837

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   hypothetical protein, conserved
   

   Use

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. D18_Ld_100590

   Plasmid

   #135834

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   hypothetical protein, unknown function
   

   Use

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. D25_Ld_190160

   Plasmid

   #135841

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   hypothetical protein, conserved
   

   Use

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. D27_Ld_240060

   Plasmid

   #135843

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   hypothetical protein, conserved
   

   Use

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. D20_Ld_140560

   Plasmid

   #135836

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   hypothetical protein, unknown function
   

   Use

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. D16_Ld_090240

   Plasmid

   #135832

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   hypothetical protein, conserved
   

   Use

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. D15_Ld_061240

   Plasmid

   #135831

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   thioredoxin, putative
   

   Use

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. D13_Ld_050900

   Plasmid

   #135829

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   surface antigen- like protein
   

   Use

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. D12_Ld_040370

   Plasmid

   #135828

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   hypothetical protein
   

   Use

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. D11_Ld_211610

    Plasmid

    #135827

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    hypothetical protein, conserved
    

    Use

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. D6_Ld_100150

    Plasmid

    #135822

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    nucleoside phosphatase, putative
    

    Use

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. D65_Ld_051210

    Plasmid

    #135878

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    surface antigen-like protein
    

    Use

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. D3_Ld_030930

    Plasmid

    #135819

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    multicopper oxidase, putative
    

    Use

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. D1_Ld_290860

    Plasmid

    #135817

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    cysteine peptidase C (CPC)
    

    Use

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. D10_Ld_292250

    Plasmid

    #135826

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    C-8 sterol isomerase-like protein
    

    Use

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. D17_Ld_090870

    Plasmid

    #135833

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    hypothetical protein, conserved
    

    Use

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. D8_Ld_340400

    Plasmid

    #135824

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    hypothetical protein, unknown function
    

    Use

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSicoR-ADAM29i2

    Plasmid

    #59607

    Purpose

    Expression of shRNA against human ADAM29

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    ADAM29 (ADAM29 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Oct. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

19. ABE-RA2.1

    Plasmid

    #208300

    Purpose

    Expresses ABE-RA2.1 in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA-RA2.1-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P48A, R51H, D108G, K110R, H122R, M126I, N127K

    Promoter

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNCMV 18E6no*

    Plasmid

    #37456

    Purpose

    Expression of HPV 18E6no* in mammalian cells

    Depositor

    Karl Munger

    Article

    Spangle et al J Virol. 2012 May 2.

    Insert

    HPV 18E6no*
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    V44A

    Promoter

    CMV

    Available Since

    Aug. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

21. pCD86-mCherry2

    Plasmid

    #190747

    Purpose

    Expression mCherry2-tagged CD86 in mammalian cells.

    Depositor

    Dave Piston

    Article

    Foust et al bioRxiv 2021.11.03.467169

    Insert

    CD86 (CD86 Human)
    

    Use

    Tags

    mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHdCas9-mammalian

    Plasmid

    #138415

    Purpose

    Expression of Himar-dCas9 in mammalian cells

    Depositor

    Harris Wang

    Article

    Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

    Insert

    Himar-dCas9
    

    Use

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pNCMV 5E6

    Plasmid

    #37459

    Purpose

    Expression of HPV 5E6 in mammalian cells

    Depositor

    Karl Munger

    Article

    Spangle et al J Virol. 2012 May 2.

    Insert

    HPV 5E6 (E6 HPV)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

24. pNCMV 6bE6

    Plasmid

    #37457

    Purpose

    Expression of HPV 6bE6 in mammalian cells

    Depositor

    Karl Munger

    Article

    Spangle et al J Virol. 2012 May 2.

    Insert

    HPV 6bE6 (E6 HPV)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

25. pMM95

    Plasmid

    #176615

    Purpose

    Extracellular domain truncated. Used to test SIRPA exclusion from the phagocytic synpase.

    Depositor

    Meghan Morrissey, Ron Vale

    Article

    Morrissey et al Immunity. 2020 Aug 18;53(2):290-302.e6.

    Insert

    FRBext-GFPint
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG-HA-LMW-FGF2

    Plasmid

    #157663

    Purpose

    Expresses FGF2 in mammalian cell

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Fibroblast growth factor 2 (FGF2 Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    deleted amino acid 1-134

    Promoter

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. His-Precission-NEDD4L_C942S_pCDNA3.1(+)

    Plasmid

    #187729

    Purpose

    Express the catalytically inactive NEDD4L FL in mammalian cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L FL with C874 mutated to a serine (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Mammalian

    Mutation

    Cys874 mutated to a serine

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHis-LMW_FGF2

    Plasmid

    #157657

    Purpose

    Expresses FGF2 in E.coli cell

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Fibroblast growth factor 2 (FGF2 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    changed Cystein 211,229 to serine, deleted amino …

    Promoter

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFastBac1-NEDD4L_ΔC2 (157)

    Plasmid

    #187720

    Purpose

    Express GST-tagged NEDD4L ΔC2 (157) in insect cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 157-975 (NEDD4L Human)
    

    Use

    Tags

    GST-HRV 3C site

    Expression

    Insect

    Mutation

    deletion of residues 1-156

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28a(+)-NEDD4L_W4-HECT

    Plasmid

    #187710

    Purpose

    Express his-tagged NEDD4L W4-HECT in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 543-975 (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-542

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGEX6P1-NEDD4L_WW34

    Plasmid

    #187718

    Purpose

    Express GST-tagged NEDD4L WW34 tandem domains in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 492-581 (NEDD4L Human)
    

    Use

    Tags

    GST-HRV 3C site

    Expression

    Bacterial

    Mutation

    only residues 492-581

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGEX6P1-NEDD4L_HECT

    Plasmid

    #187719

    Purpose

    Express GST-tagged NEDD4L HECT domain in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 594-975 (NEDD4L Human)
    

    Use

    Tags

    GST-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-593

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. AAV-hSyn-EGFP-8X2C (AAV GABA mAGNET)

    Plasmid

    #114214

    Purpose

    AAV vector that targets expression to GABAergic inhibitory neurons in rodent cortex with 98% selectivity

    Depositor

    Xue Han

    Article

    Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    AAV and Mouse Targeting

    Tags

    None

    Expression

    Mammalian

    Mutation

    None

    Promoter

    human synapsin (hSyn)

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pETduet+p300HAT

    Plasmid

    #157793

    Purpose

    Expression of p300 HAT domain

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    p300HAT (EP300 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV 3xFLAG-GABARAPL2 WT

    Plasmid

    #123103

    Purpose

    Expresses 3xFLAG-GABARAPL2 wild-type in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSR646

    Plasmid

    #65215

    Purpose

    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-6 cap gene in Sf9 insect cells

    Depositor

    Robert Kotin

    Article

    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

    Inserts

    modified AAV-2 rep78
    modified AAV-6 cap
    

    Use

    AAV

    Tags

    Expression

    Insect

    Mutation

    Promoter

    P10 and polyhedrin

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMP597

    Plasmid

    #134999

    Purpose

    Inducible ZF-m6A Writer. Use abscisic acid (ABA) to direct GATC methylation to ZF binding sites. pMinCMV-NLS-ABI1cs-ZF-NLS-P2A-Dam(N132A)-PYL1cs-HA pGK-BlastR

    Depositor

    Ahmad Khalil

    Article

    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.

    Insert

    pMinCMV-NLS-ABI1cs-ZF-NLS-P2A-Dam(N132A)-PYL1cs-HA pGK-BlastR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. FUS_anion

    Plasmid

    #98656

    Purpose

    Expresses untagged FUS AA 1-163

    Depositor

    Nicolas Fawzi

    Article

    Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. hKDR

    Plasmid

    #83436

    Purpose

    human VEGFR2 with V5 and tGFP tag

    Depositor

    Christie Thomas

    Article

    Raikwar et al Am J Physiol Cell Physiol. 2018 Aug 1;31

    Insert

    human VEGFR2 (KDR Human)
    

    Use

    Tags

    V5 and turboGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSR660

    Plasmid

    #65216

    Purpose

    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cells

    Depositor

    Robert Kotin

    Article

    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

    Inserts

    modified AAV-2 rep78
    modified AAV-8 cap
    

    Use

    AAV

    Tags

    Expression

    Insect

    Mutation

    Promoter

    P10 and polyhedrin

    Available Since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pSR651

    Plasmid

    #65213

    Purpose

    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-1 cap gene in Sf9 insect cells

    Depositor

    Robert Kotin

    Article

    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

    Inserts

    modified AAV-2 rep78
    modified AAV-1 cap
    

    Use

    AAV

    Tags

    Expression

    Insect

    Mutation

    Promoter

    p10 and polyhedrin

    Available Since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pRK5RS-HERCD533

    Plasmid

    #65224

    Purpose

    Expression of dominant negative EGFR in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Redemann et al Mol Cell Biol. 1992 Feb;12(2):491-8.

    Insert

    HERCD533 (EGFR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted AA 654-1186

    Promoter

    CMV

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

43. pHR RPS27A-3xFlag_IRES-GFP

    Plasmid

    #198390

    Purpose

    RPS27A (ubiquitin/ribosomal protein eS31 fusion) expression

    Depositor

    Jack Taunton

    Article

    Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

    Insert

    RPS27A (RPS27A Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET30a-SRAP-delCys2

    Plasmid

    #84568

    Purpose

    Encodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of catalytic Cys2

    Depositor

    Douglas Feldman

    Article

    Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

    Insert

    Srap1 (Hmces Mouse)
    

    Use

    Tags

    6xHis and S-Tag

    Expression

    Bacterial

    Mutation

    deletion of Cys2

    Promoter

    T7

    Available Since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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45. pTK165_Mem-mCherry-4.1G-CTD

    Plasmid

    #46362

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    4.1G C-terminal domain (EPB41L2 Human)
    

    Use

    Tags

    Mem (N-terminal 20 amino acids of neuromodulin fo…

    Expression

    Mammalian

    Mutation

    C-terminal domain from 886-1005 amino acids

    Promoter

    CMV

    Available Since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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46. PCDH-bio-His

    Plasmid

    #53336

    Purpose

    Expresses full-length Protocadherin alpha-2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    PCDH (PCDHA2 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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47. pcDNA3.1-flag-IARS2-Δ1-48

    Plasmid

    #232341

    Purpose

    Expresses human IARS2 with mito-location signal replaced by flag tag in mammalian cells, also carrying synonymous mutations described in Addgene #232340

    Depositor

    Quanfu Ma

    Insert

    IARS2 (IARS2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-48 (indicated as Mito-signal in UniPro…

    Promoter

    CMV

    Available Since

    June 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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48. pAM94

    Plasmid

    #226686

    Purpose

    GGG-TitinV13PV15P-23-K-9-C-25-intein-CBD

    Depositor

    Andreas Martin

    Article

    Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

    Insert

    titin (TTN Human)
    

    Use

    Tags

    GyrA inteine- Chitin Binding domain and cyclin B

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. pAM99

    Plasmid

    #226691

    Purpose

    TitinV15P-23-K-9-C-1

    Depositor

    Andreas Martin

    Article

    Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

    Insert

    titin (TTN Human)
    

    Use

    Tags

    GyrA inteine- Chitin Binding domain and cyclin B

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pAM97

    Plasmid

    #226689

    Purpose

    GGG-Titinv15P-23-K-9-K-9-K-8-C-25

    Depositor

    Andreas Martin

    Article

    Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

    Insert

    titin (TTN Human)
    

    Use

    Tags

    GyrA inteine- Chitin Binding domain and cyclin B

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge