We narrowed to 6,447 results for: nls
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Plasmid#154230PurposeEncodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter CP_nirA (no QUAS control)DepositorInsertp40S:QfDBD-VP16AD-GFP-NLS
UseSynthetic Biology; Fungal expressionTagsGFP-NLS; DsRed-SKLExpressionBacterialPromoterp40S (AN0465 A. nidulans)Available SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVE2_10_p40S_QfDBD_VP16AD_cp_penDE_DsRed_0xQUAS
Plasmid#154228PurposeEncodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter CP_penDE (no QUAS control)DepositorInsertp40S:QfDBD-VP16AD-GFP-NLS
UseSynthetic Biology; Fungal expressionTagsGFP-NLS; DsRed-SKLExpressionBacterialPromoterp40S (AN0465 A. nidulans)Available SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLM2_33_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_0xQUAS
Plasmid#154224PurposeEncodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter CP_pcbC (no QUAS control)DepositorInsertp40S:QfDBD-VP16AD-GFP-NLS
UseSynthetic Biology; Fungal expressionTagsGFP-NLS; DsRed-SKLExpressionBacterialPromoterp40S (AN0465 A. nidulans)Available SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-en15FnCas9
Plasmid#201948PurposeMammalian expression plasmid of enhanced activity FnCas9 variant en15FnCas9 with T2A-EGFP and cloning backbone for sgRNADepositorInsert3xHA-NLS-en15FnCas9-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianMutationE1603H on FnCas9PromoterCbhAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-dSpCas9
Plasmid#201953PurposeMammalian expression plasmid of dead SpCas9 with T2A-EGFP and cloning backbone for sgRNADepositorInsert3xHA-NLS-dSpCas9-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianMutationD10A and H840A on SpCas9PromoterCbhAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-nSpCas9-NG
Plasmid#171692PurposeExpresses nSpCas9-NG in mammalian cellsDepositorInsertnSpCas9-NG
UseCRISPRExpressionMammalianMutationD10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1…PromoterEF1aAvailable SinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
A3A-PBE-NG
Plasmid#138141PurposeTargeted C-to-T in rice protoplastsDepositorInsertAPOBEC3A-nCas9-NG-UGI-NLS
ExpressionPlantAvailable SinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
PABE-2
Plasmid#115623PurposeTargeted A to G in wheat plants or monocotyledons protoplastsDepositorInsertwtTadA-TadA7.10-nCas9-NLS
UseCRISPRExpressionPlantAvailable SinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
3xFNLDD/pMXs-puro
Plasmid#49536PurposeExpresses 3xFNLDD in mammalian cellsDepositorInsert3xFNLDD
UseRetroviralTags3xFLAG tag and NLS (nuclear localization signal)ExpressionMammalianPromoterLTRAvailable SinceDec. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-sadCas9-VP64
Plasmid#115790PurposeAn AAV vector expressing S. aureus dCas9 fused to VP64.DepositorInsertSa-dCas9
UseAAVTagsNLS and NLS-VP64ExpressionMammalianPromoterCMVAvailable SinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag NtDRMcd (no NLS) SUP
Plasmid#115490PurposeCRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to the SUPERMAN locus with two guide RNAsDepositorInsertg2_U6_g1_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8547 PB-PGK-dLbCpf1-2XNLS-miniVPR-mCherry-pA
Plasmid#140220PurposeConstitutive PGK driven expression of full length dCpf1 (Cas12a) -miniVPR activatorDepositorInsertdLbCpf1
UseCRISPRTagsminiVPR-mCherryExpressionMammalianMutationD832APromoterPGKAvailable SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
BM00369
Plasmid#155338PurposeBinary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling GUS. It constitutively expresses dsRed and a plant selection cassette.DepositorInsertRB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-dsRed-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-GUS-POpto_LB
UseSynthetic BiologyExpressionPlantAvailable SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
p1xNLS-FRB-mCherry-hsp86-BSD
Plasmid#85795PurposeExpression of 1xNLS mislocaliser in P. falciparumDepositorInsert1xNLS Mislocaliser
UseP. falciparum expressionTagsFRB*, mCherryPromoterPfhsp86Available SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
NLS-SuperNova
Plasmid#131858PurposeExpresses SuperNova fused to nuclear localization signal (NLS)DepositorInsertSuperNova
TagsMyc tag and c-myc nuclear localization signal (NL…ExpressionMammalianPromotercmvAvailable SinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
3xFNLDD/pMXs-IG
Plasmid#48972PurposeExpresses 3xFNLDD in mammalian cellsDepositorInsert3xFNLDD
UseRetroviralTags3xFLAG tag and NLS (nuclear localization signal)ExpressionMammalianPromoterLTRAvailable SinceDec. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG
Plasmid#138566PurposeExpresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationL1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…PromoterEFSAvailable SinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
JAB3479
Plasmid#190417PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XAO-2XPO::TetR-NLS-19St_proT::GFPint-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_6XAO-2XPO::TetR-NLS-19St_proT::GFPint-ADHt
ExpressionPlantAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2602
Plasmid#190389PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_4XAmtRO::GFPint-NLS-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_4XAmtRO::GFPint-NLS-ADHt
ExpressionPlantAvailable SinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP
Plasmid#188767PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionPromoterSFFVAvailable SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only