We narrowed to 30,996 results for: uros

1. TetO-FUW-pgk-puro

   Plasmid

   #85747

   Purpose

   tetracyline-inducible lentiviral vector with no insert for use in mammalian cells

   Depositor

   Emily Dykhuizen

   Article

   Chowdhury et al PLoS One. 2016 Apr 21;11(4):e0153718. do

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   none

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA4-TO-Puromycin-mVenus-MAP

   Plasmid

   #44118

   Depositor

   Dannel McCollum

   Article

   Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

   Type

   Empty backbone

   Use

   Tags

   mVenus-MAP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1-puro-Nono-sh1

   Plasmid

   #127650

   Purpose

   Knock-down of human NONO

   Depositor

   Nicolas Manel

   Article

   Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

   Insert

   NONO shRNA (NONO Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV-puro-N3FLAG-MYB-FL

   Plasmid

   #105596

   Purpose

   retrovirally express mouse MYB with 3*FLAG tag at N terminal and puro resistance

   Depositor

   Christopher Vakoc

   Article

   Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

   Insert

   Full length MYB (mouse) (Myb Mouse)
   

   Use

   Retroviral

   Tags

   3*FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV-LTR

   Available since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1-puro-Nono-sh5

   Plasmid

   #127651

   Purpose

   Knock-down of human NONO

   Depositor

   Nicolas Manel

   Article

   Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

   Insert

   NONO shRNA (NONO Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. AP1muA-SNAP-V5-polyA-Puro HR

   Plasmid

   #229680

   Purpose

   Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with SNAP tag and a V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   genomic sequence AP1M1 locus (AP1M1 Human)
   

   Use

   CRISPR

   Tags

   SNAP, V5

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPOTv6-puro-3Myc:mNG:3Myc

   Plasmid

   #179803

   Purpose

   Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

   Depositor

   Keith Gull, Samuel Dean

   Article

   Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

   Insert

   mNG
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   read through

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ki67∆FHA-mNeonGreen-IRESpuro2

   Plasmid

   #183739

   Purpose

   Expresses Ki67∆FHA-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67_lacking FHA (MKI67 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Mutation

   MKI67(lacking FHA)

   Promoter

   CMV

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-PCNA-IRES-puro2b

   Plasmid

   #26461

   Depositor

   Daniel Gerlich

   Article

   Held et al Nat Methods. 2010 Sep . 7(9):747-54.

   Insert

   PCNA (PCNA Human)
   

   Use

   Tags

   pEGFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Dec. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-C-mScarlet-Puro-H2BC11

    Plasmid

    #207761

    Purpose

    Donor template for mScarlet-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a mScarlet-Puro Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. Ki67 (8 repeats+LR)-mNeonGreen-IRESpuro2

    Plasmid

    #183741

    Purpose

    Expresses Ki67 (8 repeats+LR)-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 LR domain + 8 Ki-67 repeats (MKI67 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    MKI67 LR domain + 8 Ki-67 repeats

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. plentiCRISPR-v2 Puro EIF2AK4/GCN2_sgRNA

    Plasmid

    #218528

    Purpose

    sgRNA targeting human EIF2AK4/GCN2

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    EIF2AK4 (EIF2AK4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmAG-W

    Plasmid

    #67976

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/mAG markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2AmAG cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. LC201: pMVP/PB/Puro-DEST

    Plasmid

    #121874

    Purpose

    pMVP destination vector, empty PiggyBac transposon vector backbone w/ Puro selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Piggybac transposon, pmvp gateway destination vec…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. 3xFLAG-dCas9/pMXs-puro

    Plasmid

    #51240

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAC94-pmax-dCas9VP160-2A-puro

    Plasmid

    #48226

    Purpose

    dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro
    

    Use

    CRISPR

    Tags

    2A-puro, HA Tag, and VP160

    Expression

    Mammalian

    Mutation

    D10A;H840A (catalytically inactive)

    Promoter

    CAGGS

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH-DCX-TurboID-V5-puro

    Plasmid

    #190742

    Purpose

    A lentiviral plasmid encoding DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)

    Depositor

    Jeremy Baskin

    Article

    Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

    Insert

    DCX-TurboID-V5 (DCX Human, DCX is human doublecortin; TurboID is engineered BirA from E.coli)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. neurod1:Gal4; cmlc2:EGFP

    Plasmid

    #173478

    Purpose

    Gal4 is driven by -5kb neurod1 promoter. The vector contains a cmlc2:EGFP marker

    Depositor

    John Rawls

    Article

    Ye et al Cell Host Microbe. 2021 Feb 10;29(2):179

    Insert

    Gal4
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-FNLSNG-P2A-Puro

    Plasmid

    #136900

    Purpose

    Lentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    FNLSNG
    

    Use

    Lentiviral

    Tags

    FLAG-NLS and NLS

    Expression

    Mutation

    L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T1337R

    Promoter

    EF1s

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXs-puro Vps34-GFP

    Plasmid

    #38268

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

    Insert

    Phosphatidylinositol 3-kinase catalytic subunit type 3 (PIK3C3 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only