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pAD100-CHIP-myc-His

Plasmid

#236054

Purpose

Mammalian expression of human CHIP with a C-terminal myc-His tag

Depositor

Jonathan Schisler

Article

Stewart et al bioRxiv 2025.02.17.637997

Insert

CHIP (STUB1 Human)

Tags

myc-His

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-hWIPI4-3×FLAG

Plasmid

#215512

Purpose

Expresses FLAG tagged human WIPI4.

Depositor

Noboru Mizushima

Article

Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

Insert

WD-repeat protein interacting with phosphoinositides 4 (WDR45 Human)

Use

Retroviral

Tags

3×FLAG

Expression

Mammalian

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pFH3.2_mTagBFP2-MS2x12

Plasmid

#205558

Purpose

Express mTagBFP2-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mTagBFP2

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag beta-2-adrenergic-receptor

Plasmid

#14697

Depositor

Robert Lefkowitz

Article

Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

Insert

B2AR (ADRB2 Human)

Tags

Flag

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

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pET28a-mCherry-CNA35

Plasmid

#61607

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with mCherry

Depositor

Maarten Merkx

Article

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

mCherry and CNA35

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCF525-mTagBFP2_EF1a-Hygro-P2A-mTagBFP2_lenti

Plasmid

#138315

Purpose

Lenti expression of mTgBFP2

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

mTAGBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti_CRISPRi_sgAR2

Plasmid

#167001

Purpose

Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

AR Promoter sgRNA 2 (AR Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti_CRISPRi_sgAR1

Plasmid

#167000

Purpose

Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

AR Promoter sgRNA 1 (AR Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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Cbh_v5 AAV-CBE C-terminal

Plasmid

#137176

Purpose

AAV genome: expresses the C-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-CBE C-terminal; U6-protospacer

Use

AAV

Promoter

Cbh

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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AV44_pCAG.Cas9-D10A.gRNA.S1

Plasmid

#199258

Purpose

Expression construct encoding SpCas9-D10A nickase and an AAVS1-targeting guide RNA

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Inserts

SpCas9-D10A nickase
AAVS1-targeting gRNA

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

D10A

Promoter

CAG promoter and RNA polymerase III promoter for …

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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