161,123 results

1. pCMV BiP-Myc-KDEL-wt

   Plasmid

   #27164

   Purpose

   Mammalian expression of human Bip with Myc tag near the C-terminus, retaining the C-terminal KDEL ER localization sequence

   Depositor

   Ron Prywes

   Article

   Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

   Insert

   BiP (HSPA5 Human)
   

   Use

   Tags

   KDEL and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNQL001-WAPL-AID[71-114]-eGFP-FRT-Neo/Kan-FRT targeting construct

   Plasmid

   #175549

   Purpose

   Targeting vector to introduce an AID-eGFP cassette at the mouse WAPL locus using Neomycin/Kanamycin selection. Auxin-inducible degron system.

   Depositor

   Elzo de Wit

   Article

   Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

   Insert

   WAPL (Wapl Mouse)
   

   Use

   Mouse Targeting

   Tags

   AID[71-114]-eGFP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAM2314-RbcS-mNG

   Plasmid

   #165582

   Purpose

   Cyanobacterial carboxysome reporter with mNeonGreen driven by native rbcS promoter

   Depositor

   Daniel Ducat

   Article

   Sakkos et al ACS Synth Biol. 2021 Jul 16;10(7):1667-1

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Prbcs

   Available since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. H2B-mGarnet2

   Plasmid

   #104312

   Purpose

   codes for a fusion of fluorescent protein mGarnet2 and human histon 2B (H2B), makes the H2B visible in live cells

   Depositor

   Ulrich Nienhaus

   Article

   Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97

   Insert

   H2B-mGArnet2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpCas9 TadCBEd

   Plasmid

   #193835

   Purpose

   Express TadCBEd (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-CDd-SpCas9 D10A-2xUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiCRISPR v2 hBAX

   Plasmid

   #129580

   Purpose

   CRISPR deletion of human BAX

   Depositor

   Stephen Tait

   Article

   Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

   Insert

   gRNA vs human BAX (BAX Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-EF1a-EGFP-RAB7A-IRES-Puromycin

   Plasmid

   #133027

   Purpose

   Expresses EGFP-tagged late endosome marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   RAB7A (RAB7A Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Human elongation factor 1 alpha

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1

   Plasmid

   #213025

   Purpose

   Expresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cells

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   LINE-1 ORF2p (1-1275)
   

   Use

   Tags

   3C-3xFlag (ORF2p)

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRRL.PPT.SFFV.MCS.IRES.dTomato

   Plasmid

   #171174

   Purpose

   Lentiviral expression vector co-expressing dTomato

   Depositor

   Dirk Heckl

   Insert

   dTomato
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSP2-96-merTetO-EFS-BLaR

    Plasmid

    #118713

    Purpose

    Contains an optimized 96-mer TetO repeat for imaging of desired loci

    Depositor

    Huimin Zhao

    Article

    Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

    Inserts

    Blasticidin resistance gene
    EFS-NS promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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