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Plasmid#112230Purpose(+)-Limonene Synthase from Citrus sinensis in pET-28a(+). N-terminal was truncated to remove amino acids 1-52 and then His-tagged for purification.DepositorInsert(+)-Limonene Synthase
UseTagsN-terminal His-tagExpressionBacterialMutationdeleted amino acids 1-52PromoterAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Tet-OsTIR1(WT)-V5
Plasmid#158663PurposeTet-OsTIR1(WT)-V5 donor for integration at the AAVS1 locusDepositorInsertTet-OsTIR1(WT) AAVS1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
2Bc-T MBP_BoMoC(ed)_6xH
Plasmid#186461PurposeRecombinant B. mori truncated R2 RT expressionDepositorInsertB. mori truncated R2 RT
UseTags6xHis and MBPExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHIV-Luciferase
Plasmid#21375Purposeself-inactivating 3rd generation lentiviral plasmid for co-expression of your gene of interest and LuciferaseDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcs2-ATF4uORF1-2-GFP-IRES-mCherry
Plasmid#226105PurposeATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)DepositorInsertatf4 (ATF4 Human)
UseTagsGFPExpressionMammalianMutationcontains uORF1-2 of ATF4, whose stability can ser…PromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE UbiC NLS-HA-stdMCP-stdHalo-RH1
Plasmid#164614PurposeLentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag and the RH1 domain of RILPL2. Used for single-molecule imaging of actin-anchored mRNA.DepositorInsertstdMCP-stdHalo-RH1
UseLentiviralTagsHA tag and NLSExpressionBacterial and MammalianMutationPromoterUbiCAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
LbABE8e
Plasmid#138504PurposeA-to-G base editorDepositorInsertecTadA(8e)-dLbCas12a
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K29
Plasmid#22903DepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK29 only, all other lysines mutated to argininesPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-CBE6b-IVT
Plasmid#215834PurposeTemplate for in vitro transcription of CBE6b (with SpCas9)DepositorInsertCBE6b-SpCas9-2xUGI
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn-fDIO-taCasp3-TEVp
Plasmid#207997PurposeInducing apoptotic ablation of neurons through a process reliant on Flp recombinaseDepositorInserttaCasp3-TEVp
UseAAVTagsExpressionMutationPromoterhSyn promoterAvailable sinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only