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Plasmid#213624PurposeBacterial expression of SUMO-tagged rat syt1 s7+DepositorInsertsyt1 s7+ (Syt1 Rat)
UseTagsSUMOExpressionBacterialMutationL171K, H198K, K236R, C277A, N333R, Y364K, I 367C,…PromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 C2AB - 4A
Plasmid#213625PurposeBacterial expression of SUMO-tagged mouse syt7 C2AB - 4ADepositorInsertsyt7 C2AB - 4A (Syt7 Mouse)
UseTagsSUMOExpressionBacterialMutationF167A, F229A, I298A, L361APromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 C2A-2A 2B
Plasmid#213626PurposeBacterial expression of SUMO-tagged mouse syt7 C2A-2A 2BDepositorInsertsyt7 C2A-2A 2B (Syt7 Mouse)
UseTagsSUMOExpressionBacterialMutationF167A, F229A, R363HPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 C2AB-2A
Plasmid#213627PurposeBacterial expression of SUMO-tagged mouse syt7 C2AB-2ADepositorInsertsyt7 C2AB-2A (Syt7 Mouse)
UseTagsSUMOExpressionBacterialMutationI298, L361APromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-FL-syt1 5W
Plasmid#213629PurposeBacterial expression of SUMO-tagged rat FL-syt1 5WDepositorInsertFL-syt1 5W (Syt1 Rat)
UseTagsSUMOExpressionBacterialMutationM173W, F231W, F234W, C277A, V304W, Y364W, I367CPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYN2_1
Plasmid#184757PurposePlasmid for genome editing by CRISPR/Cas9DepositorInsertsCas9
sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
UseSynthetic BiologyTags2xNLSExpressionBacterial and YeastMutationPromoterPGK1 and tRNA-Phe-HDV RibozymeAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-P19-EGFP
Plasmid#196609PurposesiRNA vector expressing TMV P19 and a 720-long EGFP sequence as inverted repeatDepositorInsertp19 + EGFP inverted repeat sequence
UseTags6x His tagExpressionBacterialMutationPromoterAraCAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB3278
Plasmid#190402PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-ERF2(AD)-19St_1XAmtRO(M2)::GFPint-NLS-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-ERF2(AD)-19St_1XAmtRO(M2)::GFPint-NLS-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-P19-NP
Plasmid#196608PurposesiRNA vector expressing TMV P19 and a 425bp-long NP sequence as inverted repeatDepositorInsertp19 + Influenza virus NP inverted repeat
UseTags6x His tagExpressionBacterialMutationPromoterAvailable sinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-NP
Plasmid#196607PurposeDual-Luciferase Reporter vector carrying a 425bp-long Influenza virus NP sequence in forward directionDepositorInsertnucleocapsid protein (NP) gene, partial
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
OA-1010Av2
Plasmid#176082PurposepBac-10xUAS-L596_g9608-p10-UTR-Opie2-dsRedDepositorInsertL596_g9608.t1
UseTags3xHAExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
OA-1010Bv2
Plasmid#176083PurposepBac-10xUAS-L596_g25050-p10-UTR-Opie2-dsRedDepositorInsertL596_g25050.t1
UseTags3xHAExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ChroMD-mRuby2-ST
Plasmid#171158PurposeCation channelrhodopsin ChroMD fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertChroMD
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
Donor-PIGAshort
Plasmid#153547PurposeUsed as a donor vector for the targeted correction of a mutation in PIGA exon 6DepositorInsertPIGA (a 603-bp fragment from intron 5 and exon 6)
UseDonor plasmidTagsN/AExpressionMutationNo mutationPromoterNo promoterAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Donor-PIGAmut
Plasmid#153548PurposeUsed as a donor vector for the targeted knock-in of a mutation in PIGA exon 6DepositorInsertmutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6)
UseDonor plasmidTagsN/AExpressionMutationa 3-bp mutation in exon 6PromoterNo promoterAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10AC1)_Psyn-sgRNArodA
Plasmid#149659Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30(-10AC1), PsynAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10AC2)_Psyn-sgRNArodA
Plasmid#149660Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30(-10AC2), PsynAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_Psyn-sgRNAmreB
Plasmid#149595Purposeall-in-one CRISPRi vector for targeting B. burgdorferi mreBDepositorInsertdCas9, lacI, sgRNAmreB
UseTagsExpressionBacterialMutationPromoterPflaB, PpQE30(-10TC), PsynAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_Psyn-sgRNAftsI
Plasmid#149591Purposeall-in-all CRISPRi vector for targeting B. burgdorferi ftsIDepositorInsertdCas9, lacI, sgRNAftsI
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30(-10TC), PsynAvailable sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_Psyn-sgRNArodA
Plasmid#149657Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30(-10TC), PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only