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pLVX-Cry2olig

Plasmid

#208780

Purpose

Inducibly expresses light activatable Cry2 domain

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Cry2 mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-hMALAT1

Plasmid

#118580

Purpose

to overexpress human MALAT1 in mammalian cells

Depositor

Jianjun Zhao

Article

Hu et al Leukemia. 2018 Mar 22. pii: 10.1038/s413

Insert

MALAT1 (MALAT1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

MSCV

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_OTmut

Plasmid

#185388

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in neurons

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Has Service

AAV1 and AAV9

Insert

GPCR activation based oxytocin control sensor GRAB_OTmut

Use

AAV

Promoter

hSyn

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti- V6.3 Ultra-Chili

Plasmid

#106173

Purpose

lentiviral CMV driven tdTomato-P2A-T2A, Blasticidin selectable

Depositor

Ewa Snaar-Jagalska

Insert

dTomato

Use

Lentiviral

Tags

T2A, P2A

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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AP1muA-TurboID-polyA-G418 HR

Plasmid

#227734

Purpose

Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells.

Depositor

Francesca Bottanelli

Article

Stockhammer et al J Cell Sci. 2024 Aug 15;137(16):jcs26195

Insert

genomic sequence AP1M1 locus (AP1M1 Human)

Use

CRISPR

Tags

Turbo-ID, V5

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-surfaceHA-IRES-Puro

Plasmid

#207321

Purpose

Lentiviral expression of synthetic HA surface receptor in Mammalian cells

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

HAtag-Myctag-PDGFRB-TM-IRES-Puro

Use

Lentiviral

Expression

Mammalian

Promoter

EF1alpha_short (EFS)

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-LaminB

Plasmid

#98830

Purpose

In vivo visualization of the nuclear envelope (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

LaminB

Tags

mTurquoise2

Expression

Mammalian

Mutation

*(see below)

Promoter

CMV

Available Since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEB-C5

Plasmid

#28213

Purpose

5 reprogramming factors (OCT4, SOX2, KLF4, c-MYC and LIN28) are expressed as a single poly-cistronic unit

Depositor

Linzhao Cheng

Article

Chou et al Cell Res. 2011 Mar;21(3):518-29. doi: 10

Insert

Oct4, Sox2, Klf4, c-Myc, Lin28 (Myc, Sox2, Pou5f1, Klf4, Lin28a Mouse)

Expression

Mammalian

Available Since

June 20, 2011

Availability

Academic Institutions and Nonprofits only

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DsRed2-pBAD

Plasmid

#54608

Purpose

Localization: Protein Expression Vector , Excitation: 558, Emission: 583

Depositor

Michael Davidson

Type

Empty backbone

Tags

6xHis and DsRed2

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pAS_4xEcoLeuT(CUA)_AnapRS

Plasmid

#140019

Purpose

Plasmid with 4xEcoLeuT(CUA) cassette and E. coli AnapRS for amber suppression and incorporation of the fluorescent ncAA Anap; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

EcoLeuRS (AnapRS)

Expression

Mammalian

Mutation

L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…

Promoter

EF1

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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