We narrowed to 4,048 results for: GAST

1. lentiCRISPR v2 puro - CRBN (#2)

   Plasmid

   #166241

   Purpose

   sgRNA (#2) to generate a knockout of human CRBN by targeting the start region of Exon1

   Depositor

   Günter Schneider

   Article

   Lier et al Bioorg Chem. 2021 Nov 20:105505. doi: 10

   Insert

   CRBN (CRBN Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUAS-ERK-YFP

   Plasmid

   #42076

   Depositor

   Norbert Perrimon

   Article

   Friedman et al Nature. 2006 Nov 9;444(7116):230-4. Epub

   Insert

   Drosophila ERK ortholog (rl Fly)
   

   Use

   Tags

   YFP

   Expression

   Insect

   Mutation

   Promoter

   Hsp70

   Available since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pUCIDT-attL1-Human ABeta-attR5

   Plasmid

   #160436

   Purpose

   Entry vector for cloning human Amyloid Beta(1-42) using 2-fragment gateway recombination

   Depositor

   Nicholas Tolwinski

   Article

   Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

   Insert

   Homo sapiens amyloid beta precursor protein (APP Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. WT (DVD)

   Plasmid

   #184316

   Purpose

   To study dynamics of G-actin using NMR spectroscopy.

   Depositor

   Michael Rosen

   Article

   Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

   Insert

   Act5C (Act5C Fly)
   

   Use

   Tags

   Hexahistidine followed by B-thymosin

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk

   Plasmid

   #131143

   Purpose

   Rescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   mNeonGreen::3XFLAG::Crk (Crk Fly)
   

   Use

   Cre/Lox; Attb recombination site

   Tags

   3XFLAG and mNeonGreen

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDam-Myc-HP1

   Plasmid

   #59221

   Purpose

   expresses Dam-myc-HP1

   Depositor

   Bas van Steensel

   Article

   van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

   Insert

   HP1 (Su(var)205 Fly)
   

   Use

   Damid

   Tags

   Dam and myc

   Expression

   Insect

   Mutation

   Promoter

   Drosophila heat shock promoter

   Available since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. KHBD00716

   Plasmid

   #39744

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG17228 (pros Fly)
   

   Use

   Gateway donr vector

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Sept. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pJC-mhc2.4kb-TALE3-VP64-P10

   Plasmid

   #104611

   Purpose

   Expresses TALE3 under the control of a 2.4kb mhc enhancer element

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Insert

   2.4kb mhc enhancer element (Mhc Fly)
   

   Use

   TALEN

   Tags

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   Jan. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. KHBD00474

   Plasmid

   #39584

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG17077 (pnt Fly)
   

   Use

   Gateway donr vector

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pAc5.1B-EGFP-DmeIF4E

    Plasmid

    #79258

    Purpose

    Expresses EGFP-tagged DmeIF4E in S2 cells

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al EMBO J. 2013 Apr 3;32(7):1052-65. doi: 1

    Insert

    eIF4E (eIF4E1 Fly)
    

    Use

    Schneider 2 cell expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Ac5 promoter

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUAS-hhN-HA

    Plasmid

    #240236

    Purpose

    Expression of hhN-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

    Depositor

    Justin Bosch, Norbert Perrimon

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    hedgehog N-terminal fragment (hh Fly)
    

    Use

    Tags

    HA-spGFP11

    Expression

    Insect

    Mutation

    aa 1-257

    Promoter

    UAS

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUb FLAG-Mts

    Plasmid

    #195065

    Purpose

    Expresses FLAG-tagged Drosophila mts protein

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    microtubule star (mts Fly)
    

    Use

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    Promoter

    Ubi-p63e

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUb FLAG-Pp2A-29B

    Plasmid

    #195066

    Purpose

    Expresses FLAG-tagged Drosophila Pp2A-29B protein

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    Protein phosphatase 2A at 29B (Pp2A-29B Fly)
    

    Use

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    Promoter

    Ubi-p63e

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAc5.1B-EGFP-DmNot1_1-1717-del1156-1169_X

    Plasmid

    #147983

    Purpose

    Insect Expression of DmNot1_1-1717-del1156-1169

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    DmNot1_1-1717-del1156-1169 (Not1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    6 silent mutations and two non silent N605S, K759…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAc5.1B-EGFP-DmNot1_1-1717-del1156-1169-R1197AN1203AF1204A_X

    Plasmid

    #147984

    Purpose

    Insect Expression of DmNot1_1-1717-del1156-1169-R1197AN1203AF1204A

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    DmNot1_1-1717-del1156-1169-R1197AN1203AF1204A (Not1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    6 silent mutations and two non silent N605S, K759…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAc5.1B-EGFP-DmNot1_1-1717-del1156-1169-RNFKGQtoAAASEA_X

    Plasmid

    #147985

    Purpose

    Insect Expression of DmNot1_1-1717-del1156-1169-RNFKGQtoAAASEA

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    DmNot1_1-1717-del1156-1169-RNFKGQtoAAASEA (Not1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    6 silent mutations and two non silent N605S, K759…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAc5.1B-EGFP-DmNot1_1-1945-K1537SG1562EQ1658A-dsRNAres_X

    Plasmid

    #147990

    Purpose

    Insect Expression of DmNot1_1-1945-K1537SG1562EQ1658A-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    DmNot1_1-1945-K1537SG1562EQ1658A-dsRNAres (Not1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    8 silent mutations and two non silent N605S, K759…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAc5.1B-EGFP-DmNot1-del1156-1169-dsRNAres_X

    Plasmid

    #147991

    Purpose

    Insect Expression of DmNot1-del1156-1169-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    DmNot1-del1156-1169-dsRNAres (Not1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    10 silent mutations and two non silent N605S, K75…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAc5.1B-lambdaN-HA-DmNot1-del1156-1169-RNFtoAAA-dsRNAres_W

    Plasmid

    #147875

    Purpose

    Insect Expression of DmNot1-del1156-1169-RNFtoAAA-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    DmNot1-del1156-1169-RNFtoAAA-dsRNAres (Not1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    10 silent mutations and two non silent N605S, K75…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAc5.1B-EGFP-DmNot1-del1156-1169-RNFtoAAA-dsRNAres_W

    Plasmid

    #147859

    Purpose

    Insect Expression of DmNot1-del1156-1169-RNFtoAAA-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    DmNot1-del1156-1169-RNFtoAAA-dsRNAres (Not1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    10 silent mutations and two non silent N605S, K75…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut-F961A_R

    Plasmid

    #147389

    Purpose

    Insect Expression of CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut-F961A

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut-F961A (gw Fly, Nematode)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Deletion of 2 AA (SD) at position 378-379 compare…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAc5.1B-lambdaN-HA-CeAIN2_254-400-DmGW182_635-861_R

    Plasmid

    #147404

    Purpose

    Insect Expression of CeAIN2_254-400-DmGW182_635-861

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    CeAIN2_254-400-DmGW182_635-861 (gw Fly, Nematode)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Deletion of 2 AA (SD) at position 378-379 compare…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAc5.1B-lambdaN-HA-CeAIN2_254-400-DmGW182_635-1384_R

    Plasmid

    #147405

    Purpose

    Insect Expression of CeAIN2_254-400-DmGW182_635-1384

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    CeAIN2_254-400-DmGW182_635-1384 (gw Fly, Nematode)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Deletion of 2 AA (SD) at position 378-379 compare…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. I282L

    Plasmid

    #184308

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I282L

    Promoter

    AOX1

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. I175L

    Plasmid

    #184322

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Mutation

    I175L

    Promoter

    AOX1

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. K118N

    Plasmid

    #184324

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Mutation

    K118N

    Promoter

    AOX1

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. I212L

    Plasmid

    #184304

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I212L

    Promoter

    AOX1

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. I327L

    Plasmid

    #184311

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I327L

    Promoter

    AOX1

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. I309L

    Plasmid

    #184317

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Mutation

    I309L

    Promoter

    AOX1

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. I330L

    Plasmid

    #184320

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Mutation

    I330L

    Promoter

    AOX1

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. I151L

    Plasmid

    #184300

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I151L

    Promoter

    AOX1

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. I369L

    Plasmid

    #184318

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Mutation

    I369L

    Promoter

    AOX1

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. I274L

    Plasmid

    #184307

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I274L

    Promoter

    AOX1

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. I122L

    Plasmid

    #184299

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I122L

    Promoter

    AOX1

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. I85L

    Plasmid

    #184298

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I85L

    Promoter

    AOX1

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. I75V

    Plasmid

    #184315

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I75V

    Promoter

    AOX1

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. I345L

    Plasmid

    #184323

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Mutation

    I345L

    Promoter

    AOX1

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. I165L

    Plasmid

    #184301

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I165L

    Promoter

    AOX1

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. I329L

    Plasmid

    #184312

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I329L

    Promoter

    AOX1

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. I341L

    Plasmid

    #184313

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I341L

    Promoter

    AOX1

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. I34L

    Plasmid

    #184296

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I34L

    Promoter

    AOX1

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. I208L

    Plasmid

    #184303

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I208L

    Promoter

    AOX1

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. I250L

    Plasmid

    #184306

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I250L

    Promoter

    AOX1

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. I248L

    Plasmid

    #184305

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I248L

    Promoter

    AOX1

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. I64L

    Plasmid

    #184321

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Mutation

    I64L

    Promoter

    AOX1

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. I192L

    Plasmid

    #184302

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I192L

    Promoter

    AOX1

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. I71L

    Plasmid

    #184297

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I71L

    Promoter

    AOX1

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pGEX-2T-CBP(1603-2678)

    Plasmid

    #183774

    Purpose

    Express GST-CBP(1603-2678) in E. coli. Purified GST-CBP(1603-2678) was used for in vitro acetylation assays.

    Depositor

    Peter Harte

    Article

    Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

    Insert

    nej (nej Fly)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pMT-FLAG-CBPΔN

    Plasmid

    #182840

    Purpose

    A 5.2 kb Drosophila CBP cDNA fragment (cut by Nhe I and Nsi I), encoding C-terminal residues 1476-3222, was inserted into the pMT-FLAG vector. Expression in S2 cells by CuSO4 induction.

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2009 Sep;136(18):3131-41. d

    Insert

    CBP cDNA fragment (cut by Nhe I and Nsi I) (nej Fly)
    

    Use

    Tags

    FLAG tag

    Expression

    Insect

    Mutation

    Promoter

    MT

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pnEA-vH_His-TEV-DSep1-msfGFP

    Plasmid

    #174494

    Purpose

    bacterial expression of Drosophila Sep1 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Insert

    Sep1 (Sep1 Fly)
    

    Use

    Tags

    His6-TEV and msfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge