We narrowed to 23,248 results for: crispr

1. pTRE3G-VP64-dCas9-VP64-ZsG

   Plasmid

   #192668

   Purpose

   Dox-inducible expression of VP64-dCas9-VP64 with 2A ZsGreen1

   Depositor

   Jeffrey Miner

   Insert

   VP64-dCas9-VP64, ZsGreen1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   TRE3G

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHR Ef1a-dCas9-BFP-KRAB

   Plasmid

   #217304

   Purpose

   lentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoter

   Depositor

   Luke Gilbert

   Insert

   dCas9-tagBFP-KRAB
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Promoter

   Ef1alpha

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX551-CMV-CjCas9

   Plasmid

   #107035

   Purpose

   AAV plasmid expressing CjCas9 under control of CMV promoter

   Depositor

   Alex Hewitt

   Insert

   CjCas9
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pY130 (pcDNA3.1-U6-huBsCpf1)

   Plasmid

   #92306

   Purpose

   Expression of humanized BsCpf1 and crRNA

   Depositor

   Feng Zhang

   Insert

   huBsCpf1
   

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Available Since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX551-miniCMV-AsCpf1

   Plasmid

   #107034

   Purpose

   AAV plasmid expressing AsCpf1 under control of miniCMV promoter

   Depositor

   Alex Hewitt

   Insert

   AsCpf1
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pY128 (pcDNA3.1-U6-huMb2Cpf1)

   Plasmid

   #92304

   Purpose

   Expression of humanized Mb2Cpf1 and crRNA

   Depositor

   Feng Zhang

   Insert

   huMb2Cpf1
   

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Available Since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pY129 (pcDNA3.1-U6-huMb3Cpf1)

   Plasmid

   #92305

   Purpose

   Expression of humanized Mb3Cpf1 and crRNA

   Depositor

   Feng Zhang

   Insert

   huMb3Cpf1
   

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Available Since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pY127 (pcDNA3.1-U6-huTsCpf1)

   Plasmid

   #92303

   Purpose

   Expression of humanized TsCpf1 and crRNA

   Depositor

   Feng Zhang

   Insert

   huTsCpf1
   

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Available Since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX551-miniCMV-CjCas9

   Plasmid

   #107033

   Purpose

   AAV plasmid expressing CjCas9 under control of miniCMV promoter

   Depositor

   Alex Hewitt

   Insert

   CjCas9
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX551-miniCMV-SaCas9

    Plasmid

    #107032

    Purpose

    AAV plasmid expressing SaCas9 under control of miniCMV promoter

    Depositor

    Alex Hewitt

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRR2

    Plasmid

    #166097

    Purpose

    Gal inducible expression of dCas9-FokI

    Depositor

    Rebecca Burgess

    Insert

    dCas9-FokI
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    spCas9-D10A, H840A

    Promoter

    Gal-inducible

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-PE2-pegRNA-tetra-com vector

    Plasmid

    #136271

    Purpose

    Expresses PE2 and Tetra-com modified pegRNA cassette

    Depositor

    Baisong Lu

    Insert

    PE2 and pegRNA cassette
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pICSL4723_ERF922

    Plasmid

    #126883

    Purpose

    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.

    Depositor

    Sophien Kamoun

    Insert

    Wheat_live_Cas9
    

    Use

    CRISPR

    Available Since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pITI1

    Plasmid

    #166094

    Purpose

    Gal inducible expression of dCas9-FokI

    Depositor

    Rebecca Burgess

    Insert

    dCas9-FokI
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    spCas9-D10A, H840A

    Promoter

    Gal-inducible

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHnS 2comp KI;Nlgn2 #2

    Plasmid

    #240303

    Purpose

    KI:Nlgn2 #2

    Depositor

    Yuki Ogawa

    Insert

    KI gRNA for Nlgn2
    

    Use

    AAV

    Mutation

    NA

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV KI;Sptbn1 Donor;smFP-V5

    Plasmid

    #240309

    Purpose

    KI:Sptbn1

    Depositor

    Yuki Ogawa

    Insert

    KI gRNA for Sptbn4
    

    Use

    AAV

    Mutation

    NA

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pU6:3-tevopreq1

    Plasmid

    #190909

    Purpose

    Expression of single epegRNA under control of Drosophila U6:3 promoter. Can be used to generate transgenic flies with vermillion+ selection.

    Depositor

    Norbert Perrimon

    Insert

    Empty Backbone
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-CMV-Dpi2Cas9-puro

    Plasmid

    #232113

    Purpose

    Expresses Dpi2Cas9 and puromycin resistance genes, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CMV-Slu1Cas9-puro

    Plasmid

    #232111

    Purpose

    Expresses Slu1Cas9 and puromycin resistance genes, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-CMV-Slu2Cas9-puro

    Plasmid

    #232112

    Purpose

    Expresses Slu2Cas9 and puromycin resistance genes, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-CMV-SeqCas9-puro

    Plasmid

    #232110

    Purpose

    Expresses SeqCas9 and puromycin resistance genes, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTniQ-Cascade

    Plasmid

    #199659

    Purpose

    type I-F Cascade over-expression

    Depositor

    IIya Finkelstein

    Insert

    TniQ cas8 cas7 cas6
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pICSL4723_ HDT701

    Plasmid

    #126886

    Purpose

    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.

    Depositor

    Sophien Kamoun

    Insert

    Wheat_live_Cas9
    

    Use

    CRISPR

    Available Since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pENTRY4_Rac5

    Plasmid

    #126897

    Purpose

    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.

    Depositor

    Sophien Kamoun

    Insert

    Wheat_live_Cas9
    

    Use

    CRISPR

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pENTRY4_ OBfold

    Plasmid

    #126901

    Purpose

    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.

    Depositor

    Sophien Kamoun

    Insert

    Wheat_live_Cas9
    

    Use

    CRISPR

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pICSL4723_ OBfold

    Plasmid

    #126889

    Purpose

    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.

    Depositor

    Sophien Kamoun

    Insert

    Wheat_live_Cas9
    

    Use

    CRISPR

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pENTRY4_ HDT701

    Plasmid

    #126898

    Purpose

    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.

    Depositor

    Sophien Kamoun

    Insert

    Wheat_live_Cas9
    

    Use

    CRISPR

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pENTRY4_ ERF922

    Plasmid

    #126893

    Purpose

    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.

    Depositor

    Sophien Kamoun

    Insert

    Wheat_live_Cas9
    

    Use

    CRISPR

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pYPQ262-ABE8e

    Plasmid

    #161523

    Purpose

    Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e mediated A-G base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

    Insert

    ABE8e-zSpCas9(D10A)
    

    Use

    CRISPR; Gateway compatible abe8e-zspcas9(d10a) en…

    Expression

    Plant

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-Sox2-CreERT2

    Plasmid

    #135618

    Purpose

    Donor vector for genomic targeting of a P2A-CreERT2 cassette to the 3' end of the mouse Sox2 locus

    Depositor

    Lin He

    Article

    Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

    Insert

    P2A-CreERT2 flanked by Sox2 homology arms (Sox2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    P2A-CreERT2

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHEE401E

    Plasmid

    #71287

    Purpose

    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    CRISPR; Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Promoter

    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Cas9/pTREX-n

    Plasmid

    #68708

    Purpose

    Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.

    Depositor

    Roberto Docampo

    Article

    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

    Insert

    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
    

    Use

    CRISPR

    Tags

    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …

    Mutation

    C4239T mutation that eliminates a BamHI restricti…

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHEE401

    Plasmid

    #71286

    Purpose

    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Inserts

    sgRNA scaffold
    zCas9
    

    Use

    CRISPR; Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Promoter

    EC1.2 promoter and U6-26p Arabidopsis U6 gene pro…

    Available Since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHEE2E-TRI

    Plasmid

    #71288

    Purpose

    Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Inserts

    sgRNA targeting TRY and CPC genes
    sgRNA targeting ETC2 gene
    zCas9
    

    Use

    CRISPR; Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Promoter

    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

    Plasmid

    #64218

    Purpose

    Expression vector for sgRNA and for Expression of Cas9 linked to BFP via T2A linked to Ad4 E1B via P2A

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA cassette
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-BFP-P2A-E1B

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6

    Plasmid

    #64222

    Purpose

    Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry linked to the Ad4 E4orf6 gene via P2A

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA cassette
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-mCherry-P2A-E4orf6

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pdCas9-DNMT3A-PuroR_hNT

    Plasmid

    #71830

    Purpose

    Expression of dCas9-DNMT3A fusion with T2A-PuroR and non-targeting control sgRNA for use in human cells

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    Non-targeting sgRNA human (DNMT3A Synthetic, S. pyogenes, Human)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    U6

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHL-H1-ccdB-mEF1a-RiH

    Plasmid

    #60601

    Purpose

    Cloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), expresses RFP and hygromycin resistance gene.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Inserts

    Red Fluorescent Protein
    Hygromycin resistance gene
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E4orf6

    Plasmid

    #64220

    Purpose

    Expression vector for sgRNA and Cas9 linked via T2A to BFP linked to the Ad4 E4orf6 gene via P2A

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA cassette
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-BFP-P2A-E4orf6

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

    Plasmid

    #64219

    Purpose

    Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked via T2A to BFP linked to the Ad4 E1B55K gene via P2A

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA targeting ROSA26-1
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-BFP-P2A-E1B

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pS34.EF1α<GFP-2A-Cas9-RC

    Plasmid

    #207404

    Purpose

    Cas9-RC and GFP from an EF1α promoter. High-performance knockin through DSB repair by homology-directed recombination (HDR) or homology-mediated end joining (HMEJ). [Lab plasmid ID: N213]

    Depositor

    Alexandros Poulopoulos

    Article

    Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

    Insert

    Cas9-RC
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GFP-2A

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pHL-EF1a-SphcCas9-iP-A

    Plasmid

    #60599

    Purpose

    Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Insert

    CRISPR Cas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Codon usage optimized for human usage

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)

    Plasmid

    #64217

    Purpose

    Expression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2A

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    hU6 promoter; BbsI sites for sgRNA
    H1 promoter; BamHI site for shRNA
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-mCherry

    Expression

    Mammalian

    Promoter

    CBh, H1, and U6

    Available Since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pYPQ262B-ABE8e

    Plasmid

    #161524

    Purpose

    Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 ABE8e mediated A-G base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

    Insert

    TadA8e-zSpRYCas9(D10A)
    

    Use

    CRISPR; Gateway compatible tada8e-zsprycas9(d10a)…

    Expression

    Plant

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pYPQ266E

    Plasmid

    #161521

    Purpose

    Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 PmCDA1 mediated C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

    Insert

    zSpRYCas9(D10A)-PmCDA1-UGI
    

    Use

    CRISPR; Gateway compatible zsprycas9(d10a)-pmcda1…

    Tags

    3X FLAG, NLS

    Expression

    Plant

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pYPQ262m

    Plasmid

    #161522

    Purpose

    Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE7.10 mediated A-G base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

    Insert

    TadA(wt)-TadA(7.10)-zSpCas9(D10A)
    

    Use

    CRISPR; Gateway compatible tada(wt)-tada(7.10)-zs…

    Expression

    Plant

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pCDF-casBCDE(g8)

    Plasmid

    #89727

    Purpose

    Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing 33-nt spacer (referred to as 'wt') matching a protospacer in the M13 phage genome.

    Depositor

    Konstantin Kuznedelov, Konstantin Severinov

    Article

    Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

    Insert

    CRISPR array
    

    Expression

    Bacterial

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. AAV Intein-C-SpyCas9, CMV-AcrIIA4-2xmiR-122 target sites

    Plasmid

    #120295

    Purpose

    AAV Vector for expression of C-terminal SpyCas9 fragement with split-intein and a CMV-driven AcrIIA4 with two miR-122 binding sites

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    C-terminal fragment of SpyCas9
    

    Use

    AAV and CRISPR

    Tags

    split-intein

    Expression

    Mammalian

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCrAb-km

    Plasmid

    #214199

    Purpose

    Expresses crRNA for genome editing in Acinetobacter baumannii

    Depositor

    Yu Wang

    Article

    Wang et al Curr Issues Mol Biol. 2024 Jan 5;46(1):5

    Insert

    crRNA-HDV
    

    Use

    CRISPR

    Promoter

    J23119

    Available Since

    June 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pKm-HCBE

    Plasmid

    #170333

    Purpose

    a modularized single-plasmid cytidine base editing (C-to-T) tool for Sinorhizobium meliloti

    Depositor

    Deqiang Duanmu

    Article

    Wang et al Front Microbiol. 2021 Jun 18;12:686008.

    Insert

    Rhizobium codon-optimized CBEs
    

    Use

    CRISPR

    Mutation

    Cas9n-D10A

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge