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UASGAL-CU2-Pleum

Plasmid

#60328

Purpose

Contains a promoter comprised of derivatives from native promoters galactose, cyc and leucine.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

Nov. 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pSH1/M-Fv'-Fvls-E

Plasmid

#15273

Depositor

David Spencer

Article

Fan et al Hum Gene Ther. 1999 Sep 20. 10(14):2273-

Insert

FKBP12(V36) (FKBP1A Human)

Tags

HA epitope and Myristoylation-targeting domain c-…

Expression

Mammalian

Mutation

FKBP12 F36-->V 1st FKBP uses "wobbled&qu…

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
UASGAL-PCYC

Plasmid

#60335

Purpose

Contains a promoter of activating sequence derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

Nov. 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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sgRNA3_MYOD1

Plasmid

#64138

Purpose

Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_MYOD1 (MYOD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pBR322 hCDK4 promoter

Plasmid

#16571

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

CDK4 promoter (CDK4 Human)

Expression

Bacterial

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
MDH1-PGK-FLAG-TAT-86-A

Plasmid

#35977

Depositor

Susana Valente

Article

Mediouni et al Curr HIV Res. 2015;13(1):64-79.

Insert

transactivating protein (tat Human Immunodeficiency Virus)

Use

RNAi and Retroviral

Tags

Flag

Expression

Mammalian

Mutation

From the "A" HIV clade

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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hSTARR-seq_OCT4 3UTR vector

Plasmid

#99295

Purpose

Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Hstarr-seq screening vector

Expression

Mammalian

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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hSTARR-seq_mCP vector

Plasmid

#99291

Purpose

Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Hstarr-seq screening vector

Expression

Mammalian

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pMU103

Plasmid

#73934

Purpose

This is hpRNA construct for gene silencing in soybean seeds. Soybean seed specific promoter, Glycinin gene promoter, is used.

Depositor

Zhanyuan Zhang

Article

Flores et al Transgenic Res. 2008 Oct;17(5):839-50. d

Insert

bar and GUS cassettes

Expression

Plant

Promoter

35S

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pX330_sgACTA2

Plasmid

#63712

Purpose

Express sgACTA2 in mammalian cells.

Depositor

Stanley Qi

Article

Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

Insert

ACTA2 (ACTA2 Mouse, Human)

Expression

Mammalian

Promoter

U6

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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Short fibulin-3-V5 pcDNA4

Plasmid

#29702

Depositor

Mariano Viapiano

Article

Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

Insert

EFEMP1 (EFEMP1 Human)

Tags

V5

Expression

Mammalian

Mutation

lacks N-terminus of EFEMP1 (amino acids 1-105)

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
gamma2 CR shRNA (shRNA2)

Plasmid

#120794

Purpose

shRNA targeting the coding region of the gamma2 mRNA

Depositor

Angel de Blas

Article

Li et al J Neurochem. 2005 Nov;95(3):756-70. doi:

Insert

GABRG2 shRNA (Gabrg2 Rat)

Use

RNAi

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pSP64R1 SHP2 WT

Plasmid

#8325

Depositor

Ben Neel

Article

O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

Insert

SHP2 (PTPN11 Human)

Use

Xenopus expression

Available Since

June 17, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-RlACR_477-EYFP

Plasmid

#103771

Purpose

Anion channelrhodopsin from Rhodomonas lens (RHODO) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin R1ACR_477

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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Gal4pBS34-Pleum

Plasmid

#60334

Purpose

Contains a promoter of two gal binding sites coupled wiht a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

Feb. 11, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pSP64R1 SHP2 D61A

Plasmid

#8326

Depositor

Ben Neel

Article

O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

Insert

SHP2 (PTPN11 Human)

Use

Xenopus expression

Mutation

D61A (activated mutant)

Available Since

June 17, 2005

Availability

Academic Institutions and Nonprofits only
pJOE6089.4

Plasmid

#135081

Purpose

pBR322-based L-rhamnose-inducible vector.

Depositor

Josef Altenbuchner

Article

Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

Insert

P-rhaP-BAD-eGFP-Strep-tag II

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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hSTARR-seq_SCP1 vector_blocking 1

Plasmid

#99316

Purpose

Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Hstarr-seq screening vector

Expression

Mammalian

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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Gal4pBS12-Pleum

Plasmid

#60326

Purpose

Contains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

Feb. 11, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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TAL2386

Plasmid

#36795

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CYLD-TALEN_Left (Cyld Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only