We narrowed to 4,318 results for: Pet2

1. pET22-His-TEV-DOT1L

   Plasmid

   #89610

   Purpose

   Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L residues 1-416

   Depositor

   Leemor Joshua-Tor

   Article

   Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

   Insert

   Dot1L (DOT1L Human)
   

   Tags

   His-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a(+) 6xHis-TEV-IFIT1

   Plasmid

   #53557

   Purpose

   bacterial expression of IFIT1

   Depositor

   Kathleen Collins

   Article

   Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

   Insert

   IFIT1 (IFIT1 Human)
   

   Tags

   6xHIS and TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 28, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pET28_6xHis-PARP1-CAT-L713F

   Plasmid

   #173944

   Purpose

   Bacterial expression of isolated PARP1 CAT domain containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-CAT-L713F (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   L713F, V762A

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pet28a-ClbB-NRPS-NHis

   Plasmid

   #49217

   Purpose

   NRPS module of ClbB containing CAT domains with N-terminal 6His tag

   Depositor

   Emily Balskus

   Article

   Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

   Insert

   ClbB-NRPS
   

   Tags

   6x His tag and T7 tag

   Expression

   Bacterial

   Mutation

   contains aa4-1080 of reference sequence NP_754362…

   Promoter

   T7

   Available Since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-SENP6 (catalytic domain)

   Plasmid

   #16359

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   human SENP6 catalytic domain (SENP6 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino Acid 628 to 1112 catalytic domain of human …

   Available Since

   Dec. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

6. VV020: WT Arch-3 - eGFP in pET28b

   Plasmid

   #58488

   Purpose

   Expresses WT Archaerhodopsin-3 fused to eGFP (with 6x C-terminal His tag) in E. coli

   Depositor

   Adam Cohen

   Article

   Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

   Insert

   Archaerhodopsin-3
   

   Tags

   eGFP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-SENP8 (full length))

   Plasmid

   #16361

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   human SENP8 full length (SENP8 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   human SENP8 full length, see Depositor Comments

   Available Since

   Dec. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28-MBP-TEV-AICD

   Plasmid

   #69927

   Purpose

   bacterial expression of MBP-TEV-AICD

   Depositor

   Zita Balklava, Thomas Wassmer

   Article

   Currinn et al Cell Mol Life Sci. 2015 Jul 28.

   Insert

   AICD (APP Human)
   

   Tags

   MBP-TEV

   Expression

   Bacterial

   Mutation

   47 aa long

   Promoter

   T7

   Available Since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET24a HAB1 (172-511)

   Plasmid

   #177844

   Purpose

   Bacterial Expression of HAB1

   Depositor

   Karsten Melcher

   Article

   Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

   Insert

   Protein phosphatase 2C 16
   

   Tags

   6xHis-GST

   Expression

   Bacterial

   Available Since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET27b-ecDHFR-N23PP/L28F

    Plasmid

    #118584

    Purpose

    Bacterial expression of E.coli DHFR N23PP and L28F mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Insert

    DHFR
    

    Expression

    Bacterial

    Mutation

    L28 mutated to F28, N23 mutated to P23, a proline…

    Promoter

    T7

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a-SdrG_N2N3-HIS-ybbr

    Plasmid

    #101238

    Purpose

    SdrG N2 and N3 domains with C-terminal ybbr tag for covalent immobilization

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    SdrG_N2N3
    

    Tags

    H3V 3C, HIS, and ybbr

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28-His6-Ubc9-K14R

    Plasmid

    #185759

    Purpose

    Bacterial expression of His6-Ubc9-K14R

    Depositor

    Jeffrey Bode

    Article

    Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

    Insert

    SUMO-conjugating enzyme UBC9 (UBE2I Human)
    

    Tags

    Thrombin-cleavable His6-tag

    Expression

    Bacterial

    Mutation

    K14R

    Promoter

    T7

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-TGFα-GSY-SPM

    Plasmid

    #163615

    Purpose

    Expresses mature human Transforming Growth Factor alpha fused to SPM in bacterial cells for calcium-induced covalent coupling to Epidermal Growth Factor Receptor.

    Depositor

    Mark Howarth

    Article

    Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

    Insert

    TGFa-GSY-SPM (TGFA Human, Synthetic)
    

    Tags

    C-tag and His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PET28a-cpLumiLuc-Rapamycin sensor

    Plasmid

    #186292

    Purpose

    Expression Rapamycin sensor in e coli

    Depositor

    Kirill Alexandrov

    Article

    Guo et al Nat Commun. 2022 Feb 10;13(1):789. doi:

    Insert

    cpLumiLuc-Rapamycin sensor
    

    Tags

    HisTag

    Expression

    Bacterial

    Available Since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28-6His-SUMO-Nsp14

    Plasmid

    #154956

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-SUMO-Nsp14 (ORF1ab SARS-CoV-2)
    

    Tags

    6His-Thrombin-SUMO

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pET28a-SENP5 (catalytic domain)

    Plasmid

    #16358

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP5 catalytic domain (SENP5 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acid 567 to 755 catalytic domain of human S…

    Available Since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pET23-PBD(65-150)-N-Cys

    Plasmid

    #13722

    Depositor

    Klaus Hahn

    Article

    Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

    Insert

    PBD(65-150)-N-Cys (PAK1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Met-Cys-Gly-PBD(65-150)-

    Available Since

    March 28, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pET22b-mEB2-eGFP-6xHis

    Plasmid

    #130982

    Purpose

    Bacterial expression of mouse EB2-GFP-6xHis for protein purification.

    Depositor

    Anne Straube

    Article

    Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

    Insert

    EB2-GFP-6xHis (Mapre2 Mouse)
    

    Tags

    eGFP-6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28b-MBP-TEV-PWWP_DNMT3A

    Plasmid

    #186970

    Purpose

    Bacterial expression of human DNMT3A PWWP domain (residues 278–427) with His-MBP tag and TEV protease cleavage site.

    Depositor

    Brian Liau

    Article

    Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

    Insert

    DNMT3A PWWP domain (DNMT3A Human, Synthetic)
    

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    PWWP domain (residues 278–427)

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET22b 6His SpyCatcher-GRFT

    Plasmid

    #204502

    Purpose

    Bacterial expression of GRFT with N-terminal SpyCatcher fusion and GS linker

    Depositor

    Urartu Seker

    Article

    Ozkul et al Adv Mater Interfaces. 2022 Sep 13:220112

    Insert

    GRFT
    

    Use

    Synthetic Biology

    Tags

    6xHis and SpyCatcher

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge