We narrowed to 505 results for: SPY

1. SpyTag003-OspC

   Plasmid

   #159998

   Purpose

   Expresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cells

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   SpyTag003-OspC (20-209) C129S
   

   Use

   Tags

   His6-tag and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   C129S mutation improves expression level.

   Promoter

   T7

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST14-SpyCatcher EQ

   Plasmid

   #35045

   Depositor

   Mark Howarth

   Article

   Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

   Insert

   SpyCatcher EQ
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Deletion of C-terminal till I113, and point mutat…

   Promoter

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pDEST14-SpyCatcher002 EQ

   Plasmid

   #102830

   Purpose

   Bacterial expression of unreactive version of SpyCatcher002

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   SpyCatcher002 EQ
   

   Use

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   E77Q mutation prevents isopeptide bond formation

   Promoter

   T7

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpyCas9-AIDx

   Plasmid

   #135343

   Purpose

   Expression of wildtype nSpyCas9 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSpyCas9-hAIDx
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ166-iSpyMac

   Plasmid

   #149553

   Purpose

   Gateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMs

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

   Insert

   iSpyMacCas9
   

   Use

   CRISPR; Gateway compatible zispymaccas9 entry clo…

   Tags

   3X FLAG, NLS and NLS

   Expression

   Plant

   Mutation

   R221K, N394K, Protospacer interacting domain form…

   Promoter

   Available since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpyTag-GlpG

   Plasmid

   #79054

   Purpose

   Expresses SpyTagged GlpG protein

   Depositor

   James Bowie

   Article

   Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi

   Insert

   SpyTag-GlpG (glpG )
   

   Use

   Tags

   6xHis and SpyTag

   Expression

   Bacterial

   Mutation

   GlpG protein employed here corresponds to residue…

   Promoter

   Trc

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAW-SpyCsn2

   Plasmid

   #113251

   Purpose

   E. coli expression vector for S. pyogenes Csn2-MBP-His

   Depositor

   Jennifer Doudna

   Article

   Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

   Insert

   S. pyogenes Csn2
   

   Use

   Tags

   MBP-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCG-B-SpyTag-C

   Plasmid

   #87373

   Purpose

   An ORF part-containing vector harbouring the SpyTag sequence with 4 bp overhangs specific for the ‘upstream SpyPart’ position within an ORF assembly

   Depositor

   Tom Ellis

   Article

   Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

   Insert

   SpyTag
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAW-SpyCas1

   Plasmid

   #113248

   Purpose

   E. coli expression vector for His-MBP-S. pyogenes Cas1

   Depositor

   Jennifer Doudna

   Article

   Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

   Insert

   S. pyogenes Cas1
   

   Use

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAW-SpyCas2

    Plasmid

    #113249

    Purpose

    E. coli expression vector for His-MBP-S. pyogenes Cas2

    Depositor

    Jennifer Doudna

    Article

    Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

    Insert

    S. pyogenes Cas2
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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