Addgene

Showing: 141 - 160 of 286 results

Results per page:

Sort results by:

HyWnt3-ΔRep-D2:Luc

Plasmid

#192989

Purpose

Plasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D2)

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Wnt3 promoter

Use

Tags

Expression

Mammalian

Mutation

partial deletion of the Wnt3 repressor element-D2

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
HyWnt3-ΔRep-D4:Luc

Plasmid

#192991

Purpose

Plasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D4)

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Wnt3 promoter

Use

Tags

Expression

Mammalian

Mutation

partial deletion of the Wnt3 repressor element-D4

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
h3.7SP-Crtta

Plasmid

#20180

Depositor

Jeffrey Whitsett

Article

Tichelaar et al J Biol Chem. 2000 Apr 21. 275(16):11858-

Insert

h3.7SP-Crtta (SFTPC Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 8, 2010

Availability

Academic Institutions and Nonprofits only
SFTPC-T2A-Rox-EF1a-Rox-Venus-NLS repair template

Plasmid

#167943

Purpose

SFTPC reporter targeting repair template

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

SFTPC (SFTPC Human)

Use

CRISPR; Donor template

Tags

Venus

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Promoter

EF1a

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PRK5-FLAG-USP13/5-5

Plasmid

#61747

Purpose

express N-terminally FLAG tagged human chimera USP13/5-5

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

USP13/5-5 chimera (USP5, USP13 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

USP5 (1-781aa) fused to USP13 (786-864aa); G2667A…

Promoter

CMV

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET11a_3xFlag-nsp14 (SARS-CoV-2)

Plasmid

#169159

Purpose

To express SARS-CoV-2 nsp14 in E. coli

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

3xFlag-nsp5CS[VATLQ]-nsp14 (ORF1ab SARS-CoV-2)

Use

Tags

3xFlag and nsp5CS[VATLQ]: predicted nsp5 protease…

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T7

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET11a_3xFlag-nsp10 (SARS-CoV-2)

Plasmid

#169157

Purpose

To express SARS-CoV-2 nsp10 in E. coli

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

3xFlag-nsp5CS[VRLQ]-nsp10 (ORF1ab SARS-CoV-2, Synthetic)

Use

Tags

3xFlag and nsp5CS[VRLQ]: predicted nsp5 protease …

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T7

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-30 Ubwt (2)

Plasmid

#26685

Depositor

Linda Hicke

Article

French et al J Biol Chem. 2009 May 1. 284(18):12071-9

Insert

Ubiquitin

Use

Tags

His and S-tag

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2010

Availability

Academic Institutions and Nonprofits only
NEDD4L-C2

Plasmid

#25615

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

NEDD4L-C2 (NEDD4L Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains C2 domain

Promoter

Available since

Aug. 31, 2010

Availability

Industry, Academic Institutions, and Nonprofits
LHP1463 - INP52

Plasmid

#32432

Depositor

Linda Hicke

Article

Stamenova et al J Biol Chem. 2004 Apr 16;279(16):16017-2

Insert

INP52 (INP52 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Lac

Available since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only
pBS hTSP-5

Plasmid

#13002

Depositor

Jack Lawler

Article

Newton et al Genomics. 1994 Dec . 24(3):435-9.

Insert

COMP (COMP Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2006

Availability

Academic Institutions and Nonprofits only
pET28_Caspase-5-enzymatic-domain

Plasmid

#214306

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP5 (CASP5 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

aa133-434

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-Myc-Caspase-5

Plasmid

#214305

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP5 (CASP5 Human)

Use

Retroviral

Tags

Myc

Expression

Mutation

Promoter

MESV

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PRK5-FLAG-USP13/5-4

Plasmid

#61746

Purpose

express N-terminally FLAG tagged human chimera USP13/5-4

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

USP13/5-4 chimera (USP5, USP13 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

USP5 (1-716aa) fused to USP13 (722-864aa)

Promoter

CMV

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PRK5-FLAG-USP13/5-3

Plasmid

#61745

Purpose

express N-terminally FLAG tagged human chimera USP13/5-3

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

USP13/5-3 chimera (USP5, USP13 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

USP5 (1-628aa) fused to USP13 (653-864aa)

Promoter

CMV

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PRK5-FLAG-USP13/5-1

Plasmid

#61743

Purpose

express N-terminally FLAG tagged human chimera USP13/5-1

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

USP13/5-1 chimera (USP5, USP13 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

USP5 (1-185aa) fused to USP13 (197-864aa)

Promoter

CMV

Available since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
PRK5-FLAG-USP13/5-6

Plasmid

#61748

Purpose

express N-terminally FLAG tagged human chimera USP13/5-6

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

USP13/5-6 chimera (USP5, USP13 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

USP13 (1-721aa) fused to USP5 (717-859aa); D23Y i…

Promoter

CMV

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PRK5-FLAG-USP13/5-2

Plasmid

#61744

Purpose

express N-terminally FLAG tagged human chimera USP13/5-2

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

USP13/5-2 chimera (USP5, USP13 Human, USP13/5-2 chimera)

Use

Tags

FLAG

Expression

Mammalian

Mutation

USP5 (1-409aa) fused to USP13 (421-864aa)

Promoter

CMV

Available since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21b(+)_SARS-CoV-2_3CLpro-Q306A

Plasmid

#177334

Purpose

bacterial expression of active SARS-CoV-2 3C-like proteinase

Depositor

Christopher Overall

Article

Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:

Insert

SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)

Use

Tags

Factor Xa site-3xFlag-Myc-6xHis

Expression

Bacterial

Mutation

silent mutation C to T at position 10546 (elimina…

Promoter

T7

Available since

Nov. 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pET21b(+)_SARS-CoV-2_3CLpro-C145A-Q306A

Plasmid

#177335

Purpose

bacterial expression of inactive SARS-CoV-2 3C-like-C145A proteinase

Depositor

Christopher Overall

Article

Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:

Insert

SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)

Use

Tags

Factor Xa site-3xFlag-Myc-6xHis

Expression

Bacterial

Mutation

silent mutation C to T at position 10546 (elimina…

Promoter

T7

Available since

Nov. 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge