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Plasmid#173717PurposeWT EZH2 expression plasmid with 3XFLAG tagDepositorInsertEZH2 (EZH2 Human)
UseTags3XFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v1-Fc(IgG1)
Plasmid#145164PurposeMammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity variant 1DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsFc region of human IgG1ExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v1-8his
Plasmid#149269PurposeMammalian expression plasmid for his-tagged sACE2 high affinity variant 1DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsShort GSG linker and 8his tagExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v1-sfGFP
Plasmid#145172PurposeMammalian expression plasmid for sACE2-sfGFP high affinity variant 1DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsLinker GSGGSGSGG and superfolder GFPExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v1-Fc(IgA1)
Plasmid#145155PurposeMammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 1DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsFc region of human IgA1ExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag C/EBPb LAP
Plasmid#66979PurposeExpression plasmid for mouse C/EBPb LAP; adds Flag tag to the N-terminus of proteinDepositorInsertC/EBP beta (Cebpb Mouse)
UseTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SARS-CoV-2-S-ectodomain
Plasmid#235984PurposeInternally tagged SARS-CoV-2 spike ecto-domainDepositorInsertSpike protein (S SARS-CoV-2)
UseTags8xHis and Twin-Strep tag and internal Twin-Strep …ExpressionMammalianMutationaa 1-1208, D614G, RRAR684-684GSAS, and DK987-987P…PromoterCMVAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-dPlcR(K30/32L)
Plasmid#181833PurposePhospholipid binding-deficient Lyn-dPlcR negative-control plasmid.DepositorInsertLyn-dPlcR (K30L,K32L)
UseTagsN-terminal targeting sequence from Lyn kinase, dd…ExpressionMammalianMutationLysines 30 and 32 in PH domain mutated to leucine.PromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-PlcR(K30/32L)
Plasmid#181831PurposePhospholipid binding-deficient Lyn-PlcR negative-control plasmid.DepositorInsertLyn-PlcR (K30L,K32L)
UseTagsECFP, EYFP, and N-terminal targeting sequence fro…ExpressionMammalianMutationLysines 30 and 32 in PH domain mutated to leucine.PromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ER-EBFP2
Plasmid#231183PurposeExpresses control plasmid for ER-mitochondrial linkers, tagged with EBFP2, in mammalian cellsDepositorInsertER-EBFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ER-mRFP
Plasmid#231176PurposeExpresses control plasmid for ER-mitochondrial linkers, tagged with mRFP, in mammalian cellsDepositorInsertER-mRFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
(369) pcDNA3.1(+)-HA-nLaG6-(EAAAK)4-OGA(544-706)
Plasmid#168190PurposeHA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGA with residues 544-706. For use with myc-OGA(1-400) split-OGA system.DepositorInsertHA-nLag6-(EAAAK)4-OGA(544-706)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
(302) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5)
Plasmid#168192PurposeHA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGT of 4 TPRsDepositorInsertHA-LaG6-(EAAAK)4-OGT(4)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Tras heavy chain-GSG-SpyTag003
Plasmid#216292PurposeExpresses anti-HER2 Tras Heavy chain-GSG-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras FabDepositorInsertTras heavy chain-GSG-SpyTag003
UseAffinity Reagent/ AntibodyTagsHis6 tag, Signal peptide, and SpyTag003ExpressionMammalianMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcdna3.1_mouseST6galI
Plasmid#178435Purposemurine St6gal1 expression plasmidDepositorInsertSt6gal1 (St6gal1 Mouse)
UseTags6xHIS-TEV Cleavage SiteExpressionMammalianMutationCoding sequnce for amino acids 85-403PromoterCMVAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
(366) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4, K852A)
Plasmid#168189PurposeHA-tagged Ubc6E nanobody fused to an inactive truncated OGT of 4 TPRs with K852A mutationDepositorInsertHA-nUbc6E-(EAAAK)4-OGT(4,K852A)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_535-888 USP7
Plasmid#131248PurposeMammalian expression of the USP7 UBL1-3 domains with an N-terminal Myc tag.DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
UseTagsMycExpressionMammalianMutationA mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_535-775 USP7
Plasmid#131247PurposeMammalian expression of the USP7 UBL1-2 domains with an N-terminal Myc tag.DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
UseTagsMycExpressionMammalianMutationA mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CarO-BFP2-TSERex
Plasmid#124795PurposeExpresses the proton pump rhodopsin in mammalian cells under CAG promoterDepositorInsertCarO-BFP2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Mac-BFP2-TSERex
Plasmid#124793PurposeExpresses the proton pump rhodopsin in mammalian cells under CAG promoterDepositorInsertMac-BFP2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Char-BFP2-TSERex
Plasmid#124792PurposeExpresses the proton pump rhodopsin in mammalian cells under CAG promoterDepositorInsertChar-BFP2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CRISPR-Puro-sgRPL3-62
Plasmid#208647PurposepcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.DepositorInsertsgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Human, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV and U6 in tandemAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CRISPR-Puro-sgRPL3-56
Plasmid#208979PurposepcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.DepositorInsertsgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Human, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV and U6 in tandemAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Nod-BFP2-TSERex
Plasmid#124785PurposeExpresses the proton pump rhodopsin in mammalian cells under CAG promoterDepositorInsertNod-BFP2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Neo-LUC
Plasmid#214368PurposeExpression of the firefly luciferase ProteinDepositorInsertLUC
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-V5-Lin28A
Plasmid#51387DepositorInsertLin28A (LIN28A Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-flag-IARS2-Δ1-48
Plasmid#232341PurposeExpresses human IARS2 with mito-location signal replaced by flag tag in mammalian cells, also carrying synonymous mutations described in Addgene #232340DepositorInsertIARS2 (IARS2 Human)
UseTagsExpressionMammalianMutationResidues 1-48 (indicated as Mito-signal in UniPro…PromoterCMVAvailable sinceJune 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-LAP2-YFP-NLS
Plasmid#25847DepositorInsertLAP2-YFP-NLS
UseTagsHis and NLSExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
(424) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5, K852A)
Plasmid#168191PurposeHA-tagged GFP nanobody Lag6 (310 nM affinity) fused to an inactive truncated OGT of 4 TPRs with K852A mutationDepositorInsertHA-nLag6-(EAAAK)4-OGT(4,K852A)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sPDGFRalpha-Fc
Plasmid#136438PurposeMammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 FcDepositorInsertPDGFRalpha (PDGFRA Human)
UseTagsFc region of IgG1 (a.a. Asp-221 to Lys-447) and L…ExpressionMammalianMutationExtracellular domain of PDGFR-alpha (a.a. 1-524)PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only