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Showing: 281 - 320 of 1450 results

  1. sfGFP-FYN-N-10

    Plasmid
    #56294
    Purpose
    Localization: Membrane Associated Kinase, Excitation: 485, Emission: 507
    Depositor
    Michael Davidson
    Insert
    FYN (FYN Human)
    Use
    Tags
    sfGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 16, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  2. mTagGFP2-Golgi-7

    Plasmid
    #56415
    Purpose
    Localization: Golgi Complex, Excitation: 483, Emission: 506
    Depositor
    Michael Davidson
    Insert
    Golgi (B4GALT1 Human)
    Use
    Tags
    mTagGFP2
    Expression
    Mammalian
    Mutation
    aa 1-82 of NM_001497.3
    Promoter
    CMV
    Available since
    April 1, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. sfGFP-ER-5

    Plasmid
    #56483
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 485, Emission: 507
    Depositor
    Michael Davidson
    Insert
    ER (CALR Human)
    Use
    Tags
    sfGFP
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. sfGFP-Golgi-7

    Plasmid
    #56485
    Purpose
    Localization: Golgi Complex, Excitation: 485, Emission: 507
    Depositor
    Michael Davidson
    Insert
    Golgi (B4GALT1 Human)
    Use
    Tags
    sfGFP
    Expression
    Mammalian
    Mutation
    aa 1-82 of NM_001497.3
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. EGFP-Alpha-5-Integrin-10

    Plasmid
    #56423
    Purpose
    Localization: Focal Adhesions, Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Insert
    Alpha-5 Integrin (ITGA5 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 23, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. iLOV-Mito-7

    Plasmid
    #56519
    Purpose
    Localization: Mitochondria, Excitation: 450, Emission: 495
    Depositor
    Michael Davidson
    Insert
    Mito (COX8A Human)
    Use
    Tags
    iLOV
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 23, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. mWasabi-ER-3

    Plasmid
    #56501
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 493, Emission: 509
    Depositor
    Michael Davidson
    Insert
    ER (CALR Human)
    Use
    Tags
    mWasabi
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available since
    Jan. 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. mWasabi-Golgi-7

    Plasmid
    #56504
    Purpose
    Localization: Golgi Complex, Excitation: 493, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Golgi (B4GALT1 Human)
    Use
    Tags
    mWasabi
    Expression
    Mammalian
    Mutation
    aa 1-82 of NM_001497.3
    Promoter
    CMV
    Available since
    Jan. 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pUS252y

    Plasmid
    #191834
    Purpose
    Expresses fuYFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuYFP to place in other plasmid backbones.
    Depositor
    Nicholas Coleman
    Insert
    fuYFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 17, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    191834-plasmid-map-sequence-id-382126

    Enlarge

  10. mVenus-UbiquitinC-N-18

    Plasmid
    #56339
    Purpose
    Localization: Membrane , Excitation: 515, Emission: 528
    Depositor
    Michael Davidson
    Insert
    UbiquitinC (UBA52 Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    aa 2-76 of NP_003324.1
    Promoter
    CMV
    Available since
    Dec. 18, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    56339-plasmid-map-sequence-id-383948

    Enlarge

  11. mVenus-UbiquitinC-C-18

    Plasmid
    #56393
    Purpose
    Localization: Membrane, Excitation: 515, Emission: 528
    Depositor
    Michael Davidson
    Insert
    UbiquitinC (UBA52 Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    aa 2-76 of NP_003324.1
    Promoter
    CMV
    Available since
    Dec. 18, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. pUS252L

    Plasmid
    #191832
    Purpose
    Expresses fuBFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuBFP to place in other plasmid backbones
    Depositor
    Nicholas Coleman
    Insert
    fuBFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 17, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    191832-plasmid-map-sequence-id-382125

    Enlarge

  13. pUS252b

    Plasmid
    #191831
    Purpose
    Expresses fuGFPb constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFPb to place in other plasmid backbones
    Depositor
    Nicholas Coleman
    Insert
    fuGFPb
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 17, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    191831-plasmid-map-sequence-id-382128

    Enlarge

  14. pUS252s

    Plasmid
    #191833
    Purpose
    Expresses fuGFPs constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFPs to place in other plasmid backbones
    Depositor
    Nicholas Coleman
    Insert
    fuGFPs
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 17, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    191833-plasmid-map-sequence-id-382124

    Enlarge

  15. mVenus-Paxillin-KM-N-18

    Plasmid
    #56334
    Purpose
    Localization: Focal Adhesions, Excitation: 515, Emission: 528
    Depositor
    Michael Davidson
    Insert
    Paxillin KM
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Mutation to remove Kozak sequence
    Promoter
    CMV
    Available since
    Dec. 18, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. pPNT6-eGFP

    Plasmid
    #71303
    Purpose
    This vector can be used for homologous recombination and allows to obtain recombinant neomycin resistant fluorescent cells that can loose fluorescence after Cre expression
    Depositor
    Gian Luca Forni, Marco Musso
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    71303-plasmid-map-sequence-id-130367

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  18. P48

    Plasmid
    #212155
    Purpose
    Plasmid expressing 6x His tagged secondary alcohol dehydrogenase fused with superfolder green fluorescent protein at N terminus under constitutive promoter PgyrA control.
    Depositor
    Kang Zhou
    Insert
    superfolder green fluorescent protein, secondary alcohol dehydrogenase
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Promoter
    PgyrA
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212155-plasmid-map-sequence-id-420758

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  19. pDH3

    Plasmid
    #83789
    Purpose
    Useful for tagging yeast proteins with C-terminal CFP tag; G418 selection
    Depositor
    Eric Muller
    Article
    Hailey et al Methods Enzymol. 2002;351:34-49.
    Insert
    CFP
    Use
    Tags
    CFP
    Expression
    Yeast
    Mutation
    F64L, Y66W, N146I, M153T, V163A, N164H mutations …
    Promoter
    Available since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    83789-plasmid-map-sequence-id-160016

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  20. pDH5

    Plasmid
    #83775
    Purpose
    Useful for tagging yeast proteins with C-terminal YFP tag; his selection
    Depositor
    Eric Muller
    Article
    Hailey et al Methods Enzymol. 2002;351:34-49.
    Insert
    YFP
    Use
    Tags
    YFP
    Expression
    Yeast
    Mutation
    S65G, V68L, Q69K, S72A, T203Y. Also includes Q80R…
    Promoter
    Available since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    83775-plasmid-map-sequence-id-159997

    Enlarge

  23. mVenus-SiT-N-15

    Plasmid
    #56336
    Purpose
    Localization: Golgi TGN, Excitation: 515, Emission: 528
    Depositor
    Michael Davidson
    Insert
    SiT (ST6GAL1 Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    aa 1-45 of SiT
    Promoter
    CMV
    Available since
    Dec. 18, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  24. pUS252

    Plasmid
    #127674
    Purpose
    Expresses fuGFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFP to place in other plasmid backbones
    Depositor
    Nicholas Coleman
    Insert
    Free Use GFP (fuGFP)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac
    Available since
    June 24, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    127674-plasmid-map-sequence-id-251848

    Enlarge

  25. mCitrine-SiT-N-15

    Plasmid
    #56318
    Purpose
    Localization: Golgi TGN, Excitation: 516, Emission: 529
    Depositor
    Michael Davidson
    Insert
    SiT (ST6GAL1 Human)
    Use
    Tags
    mCitrine
    Expression
    Mammalian
    Mutation
    aa 1-45 of SiT
    Promoter
    CMV
    Available since
    Dec. 5, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  26. pAG Retro EGFP Neo

    Plasmid
    #234464
    Purpose
    Control retroviral vector expressing Green Fluorescent Protein. Neomycin selection.
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Green Fluorescent Protein
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 21, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234464-plasmid-map-sequence-id-464634

    Enlarge

  27. pAG Retro EGFP Puro

    Plasmid
    #234463
    Purpose
    Control retroviral vector expressing Green Fluorescent Protein. Puromycin selection.
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Green Fluorescent Protein
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 21, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234463-plasmid-map-sequence-id-464635

    Enlarge

  28. pAG Retro EGFP Hygro

    Plasmid
    #234462
    Purpose
    Control retroviral vector expressing Green Fluorescent Protein. Hygromycin selection.
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Green Fluorescent Protein
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 21, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234462-plasmid-map-sequence-id-464636

    Enlarge

  29. pcDNA3-EGFP

    Plasmid
    #13031
    Purpose
    Mammalian expression vector for cloning your gene fused to EGFP
    Depositor
    Doug Golenbock
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13031-plasmid-map-sequence-id-305137

    Enlarge

  30. GANLS-DEVD-BNES

    Plasmid
    #50835
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddGFP A and ddRFP B
    Use
    Tags
    NES sequence LALKLAGLDIGS and triplicated NLS seq…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    50835-plasmid-map-sequence-id-76278

    Enlarge

  31. mWasabi-Farnesyl-5

    Plasmid
    #56503
    Purpose
    Localization: Plasma Membrane, Excitation: 493, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    mWasabi
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available since
    Jan. 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  32. iLOV-Farnesyl-5

    Plasmid
    #56516
    Purpose
    Localization: Membrane, Excitation: 450, Emission: 495
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    iLOV
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 281 - 320 of 1450 results