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Plasmid#178422PurposeExpression of deGFP via a T7 Promoter in TXTLDepositorInsertdeGFP
UseSynthetic BiologyTags8xHis TagExpressionBacterialMutationPromoterT7MaxAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEmax(TadA E59A, TadA*E59A)
Plasmid#125649PurposepCMV-ABEmax(TadA E59A, TadA*E59A) in mammalian cellsDepositorInsertABEmax(TadA E59A TadA* E59A)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/HisMaxB-YAP2-1st&2nd WW mutant
Plasmid#19000DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTagsHisExpressionMammalianMutationTryptophan 199 changed to Alanine, and Proline 2…PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEmax(TadA E59A, TadA*V106Q)
Plasmid#125651PurposepCMV-ABEmax(TadA E59A, TadA*V106Q) in mammalian cellsDepositorInsertABEmax(TadA E59A TadA* V106Q)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEmax(TadA E59A, TadA*R47Q)
Plasmid#125657PurposepCMV-ABEmax(TadA E59A, TadA*R47Q) in mammalian cellsDepositorInsertABEmax(TadA E59A TadA* R47Q)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEmax(TadA E59A, TadA*D108Q)
Plasmid#125655PurposepCMV-ABEmax(TadA E59A, TadA*D108Q) in mammalian cellsDepositorInsertABEmax(TadA E59A TadA* D108Q)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
Anti-KCNU1/Slo3 maxi-K+ channel [N2/16R]
Plasmid#114541PurposeMammalian Expression Plasmid of anti-KCNU1/Slo3 maxi-K+ channel (Mouse). Derived from hybridoma N2/16.DepositorInsertanti-KCNU1/Slo3 maxi-K+ channel (Mus musculus) recombinant mouse monoclonal antibody (Kcnu1 Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-spCas9 ABE-Umax-ex2-rest1
Plasmid#222143PurposeAdenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafishDepositorInsertModified TadA-nSpCas9
UseCRISPR; Zebrafish expressionTagsBipartite NLSExpressionMutationPromoterT3Available sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-spCas9 ABE-Umax-ex1-rest1
Plasmid#222140PurposeAdenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafishDepositorInsertModified TadA-nSpCas9
UseCRISPR; Zebrafish expressionTagsBipartite NLSExpressionMutationPromoterT3Available sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pzk408-pmax-3xflag-dpspCas13b(AAAA)-NES
Plasmid#196828PurposeExpress dpdpCas13b with AAAA mutationDepositorInsertdpspCas13b(AAAA)
UseTags3XFLAG and NESExpressionMammalianMutationK367A, D369A and K370APromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-Slo1/BKAlpha maxi-K+ channel [L6/48R]
Plasmid#206523PurposeMammalian Expression Plasmid of anti-Slo1/BKAlpha maxi-K+ channel (Mouse). Derived from hybridoma L6/48.DepositorInsertanti-Slo1/BKAlpha maxi-K+ channel (Mus musculus) recombinant Mouse monoclonal antibody (Kcnma1 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Anti-Slo1/BKAlpha maxi-K+ channel [L6/60R-2b]
Plasmid#199406PurposeMammalian expression plasmid of Anti-Slo1/BKAlpha maxi-K+ channel [L6/60R-2b] (Rat). Derived from hybridoma L6/60.DepositorInsertAnti-Slo1/BKAlpha maxi-K+ channel (Rat) recombinant mouse monoclonal antibody (Kcnma1 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEmax(TadA E59Q, TadA* V106W)
Plasmid#126496PurposeExpresses ABEmax(TadA E59Q, TadA*V106W) in mammalian cellsDepositorInsertABEmax(TadA E59Q TadA* V106W)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEmax(TadA E59, TadA* V106W)
Plasmid#126497PurposeExpresses ABEmax(TadA E59, TadA*V106W) in mammalian cellsDepositorInsertABEmax(TadA E59 TadA* V106W)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEmax(TadA E59A, TadA*R47W)
Plasmid#125659PurposepCMV-ABEmax(TadA E59A, TadA*R47W) in mammalian cellsDepositorInsertABEmax(TadA E59A TadA* R47W)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEmax(TadA E59A, TadA*R47M)
Plasmid#125660PurposepCMV-ABEmax(TadA E59A, TadA*R47M) in mammalian cellsDepositorInsertABEmax(TadA E59A TadA* R47M)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEmax(TadA E59A, TadA*D108M)
Plasmid#125656PurposepCMV-ABEmax(TadA E59A, TadA*D108M) in mammalian cellsDepositorInsertABEmax(TadA E59A TadA* D108M)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEmax(TadA E59A, TadA*R47F)
Plasmid#125658PurposepCMV-ABEmax(TadA E59A, TadA*R47F) in mammalian cellsDepositorInsertABEmax(TadA E59A TadA* D108F)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEmax(TadA E59A, TadA*D108W)
Plasmid#125653PurposepCMV-ABEmax(TadA E59A, TadA*D108W) in mammalian cellsDepositorInsertABEmax(TadA E59A TadA* D108W)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEmax(TadA E59A, TadA*D108F)
Plasmid#125654PurposepCMV-ABEmax(TadA E59A, TadA*D108F) in mammalian cellsDepositorInsertABEmax(TadA E59A TadA* D108F)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEmax(TadA E59A, TadA*V106M)
Plasmid#125650PurposepCMV-ABEmax(TadA E59A, TadA*V106M) in mammalian cellsDepositorInsertABEmax(TadA E59A TadA* V106M)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEmax(TadA E59A, TadA*V106F)
Plasmid#125652PurposepCMV-ABEmax(TadA E59A, TadA*V106F) in mammalian cellsDepositorInsertABEmax(TadA E59A TadA* V106F)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)
Plasmid#140003PurposeCMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564)
Plasmid#140005PurposeCMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABEmax(7.10) SpCas9-NG with P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)
Plasmid#140002PurposeCMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PEmaxdeltaRNaseH-dualU6-Rosa26-twinPE-attB
Plasmid#207859PurposeAAV genome encoding C-terminal PEmaxDRNaseH and U6 expression cassettes for Rosa26 twinPE pegRNA pairsDepositorInsertNpuC-Cterm-PEmaxDRNaseH-dualU6-Rosa26-twinPE-attB
UseAAVTagsExpressionMutationSee ManuscriptPromoterCbhAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PEmaxdeltaRNaseH-dualU6-Dnmt1-PE3-loxP
Plasmid#207862PurposeAAV genome encoding C-terminal PEmaxDRNaseH and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNADepositorInsertNpuC-Cterm-PEmaxDRNaseH-dualU6-Dnmt1-PE3-loxP
UseAAVTagsExpressionMutationSee ManuscriptPromoterCbhAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637)
Plasmid#140000PurposeCAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFPDepositorInserthuman codon optimized CBE4max xCas9(3.7) with P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…PromoterCAGAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Anti-Slo1/BKAlpha maxi-K+ channel (Mouse) [L6/23R]
Plasmid#128634PurposeMammalian Expression Plasmid of anti-Slo1/BKAlpha maxi-K+ channel (Mouse). Derived from hybridoma L6/23.DepositorInsertanti-Slo1/BKAlpha maxi-K+ channel (Mouse) recombinant mouse monoclonal antibody (Kcnma1 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pzk384-pmax-PUFc-FseI-RESCUE-SalI-NES
Plasmid#196831PurposeExpress PUFc-RESCUE-SDepositorInsertPUFc-RESCUE
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pzk488-pmax-NES-PUFc-FseI[ADAR2-DD-splitN]
Plasmid#196841PurposeExpress NES-PUFc-FseI[ADAR2-DD-splitN]DepositorInsertNES-PUFc-FseI[ADAR2-DD-splitN]
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pzk491-pmax-NES-PUFc-FseI[ADAR2-DD-splitC]
Plasmid#196842PurposeExpress NES-PUFc-FseI[ADAR2-DD-splitC]DepositorInsertNES-PUFc-FseI[ADAR2-DD-splitC]
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pzk228-pmax-SgrAI[3xFLAG]-AscI-dPspCas13b(AAAA)
Plasmid#196827PurposeExpress dpdpCas13b with AAAA mutationDepositorInsertdpspCas13b(AAAA)
UseTags3XFLAGExpressionMammalianMutationK367A, D369A and K370APromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid
Plasmid#154429Purposehu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmidDepositorInserthu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid
UseCRISPR and LentiviralTagsExpressionMammalianMutationVRQR point mutations in SpCas9PromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABEmax(7.10)-xCas9(3.7)-P2A-EGFP (RTW4644)
Plasmid#140004PurposeCMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFPDepositorInserthuman codon optimized ABEmax(7.10) xCas9(3.7) with P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Anti-Slo1/BKAlpha maxi-K+ channel [L6/60R-rat IgG2a]
Plasmid#222180PurposeMammalian Expression Plasmid of anti-Slo1/BKAlpha maxi-K+ channel (Mouse). Derived from hybridoma L6/60.DepositorInsertAnti-Slo1/BKAlpha maxi-K+ channel (Mus musculus) recombinant mouse monoclonal antibody. (Kcnma1 Chimera: M. musculus (mouse)/R. norvegicus (rat))
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pzk490-pmax-NES-dpspCas13b(AAAA)-FseI[ADAR2-DD-splitC]
Plasmid#196843PurposeExpress NES-dpspCas13b(AAAA)-FseI[ADAR2-DD-splitC]DepositorInsertNES-dpspCas13b(AAAA)-FseI[ADAR2-DD-splitC]
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector
Plasmid#154430Purposehu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vectorDepositorInserthu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector
UseAAV and CRISPRTagsExpressionMammalianMutationVRQR point mutations in SpCas9PromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
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Cas9 MDC123
Plasmid#59184Purpose2x35S promoting Glycing Max codon optimized Cas9 with bar plant selectionDepositorInsertGlycine Max codon optimized Cas9
UseCRISPRTagsExpressionPlantMutationPromoter2X35SAvailable sinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
G10 Cas9 MDC123
Plasmid#59187PurposeG10 promoting Glycing Max codon optimized Cas9 (N and C terminus NLS) with bar plant selectionDepositorInsertGlycine Max codon optimized Cas9
UseCRISPRTagsExpressionPlantMutationPromoterG10Available sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG414GPD-GmCYP722C(pYL887)
Plasmid#178298PurposeExpresses GmCYP722C from G. max in Saccharomyces cerevisiae. Centromeric TRP, PGPD-GmCYP722C-TCYC1DepositorInsertGmCYP722C
UseTagsExpressionYeastMutationPromoterGAPAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUPD1:P1-MAR
Plasmid#106213PurposeProvides Glycine max P1 matrix attachment region as a level 0 GoldenBraid partDepositorInsertGlycine max P1 matrix attachment region
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC5
Plasmid#104779PurposepSC218GG-Glycine max Ubiquitin:Gateway expression cassette binary vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pEU-Gm23
Plasmid#53738PurposeUsed for in vitro transcription/translation of soybean His-tagged arginyl-tRNA synthetase in the Cell Free Sciences systemDepositorInsertarginyl-tRNA synthetase
UseSynthetic BiologyTagsHis tagExpressionMutationPromoterSP6Available sinceJuly 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
RNAseq_cal_Tuschl
Plasmid#59426PurposeEncodes sequence non-cognate to human genome for generation of RNAseq calibratorsDepositorInsertRNAseq_cal_Tuschl
UseTagsExpressionMutationPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
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