We narrowed to 4,940 results for: MLS

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pMLS7-egfp

Plasmid

#112917

Purpose

Fluorescence microscopy

Depositor

Miguel Valvano

Article

Lefebre et al Appl Environ Microbiol. 2002 Dec;68(12):

Insert

eGFP

Expression

Bacterial

Promoter

PS7 promoter and Ps7

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA h-MLSN

Plasmid

#21083

Depositor

Craig Montell

Article

Xu et al Proc Natl Acad Sci U S A. 2001 Sep 11. 9

Insert

TrpM1 (TRPM1 Human)

Tags

Myc

Expression

Mammalian

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
MLM3639

Plasmid

#42252

Purpose

Cas9 expression vector

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

Streptococcus pyogenes Cas9-3X Flag

Use

CRISPR; Zebrafish expression

Tags

3X Flag

Promoter

CMV

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

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MLS-mir-17-92-Mut20

Plasmid

#64095

Purpose

expresses a mutated version of mir-17-92 (mutation of miR-20 seed)

Depositor

Lin He

Article

Olive et al Elife. 2013 Oct 15;2:e00822. doi: 10.755

Insert

miR-17-92 (MIR17HG Human)

Expression

Mammalian

Mutation

mutation of miR-20 seed sequence

Promoter

MSCV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only
pCA24N-sso7d-ligase

Plasmid

#87745

Purpose

IPTG-inducible expression of sso7d-ligase fusion for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis and codon optimized sso7d (Sulfolobus solfa…

Expression

Bacterial

Promoter

T5-lac

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pBEN276

Plasmid

#69150

Purpose

Integration of luciferase operon in Tn7 insertion site of Gram negative bacteria

Depositor

Pierre Germon

Article

Howe et al BMC Microbiol. 2010 Jul 23;10:197. doi:

Insert

luxCDABE

Use

Luciferase

Promoter

frr

Available Since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only

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mito NAD+ biosensor

Plasmid

#186791

Purpose

NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag totarget mitochondria

Depositor

Michael Cohen

Article

Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

Insert

mito NAD+ biosensor

Use

Lentiviral

Expression

Mammalian

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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cytoplasmic NAD+ biosensor

Plasmid

#186787

Purpose

NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the cytoplasm

Depositor

Michael Cohen

Article

Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

Insert

cytoplasmic NAD+ biosensor

Use

Lentiviral

Expression

Mammalian

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pRPN265NB-enh-MNase

Plasmid

#166035

Purpose

Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

Depositor

Marc Timmers

Article

Nizamuddin et al Nucleic Acids Res. 2021 May 21;49(9):e49

Insert

Glutathione-S-Transferase/enhancer/MNase

Tags

GST

Expression

Bacterial

Promoter

lac promoter / tac promoter

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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NW30

Plasmid

#133444

Purpose

Covalently circularized nanodisc NW30 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Article

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW30

Expression

Bacterial

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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nuclear NAD+ biosensor

Plasmid

#186789

Purpose

NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the nucleus

Depositor

Michael Cohen

Article

Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

Insert

nuclear NAD+ biosensor

Use

Lentiviral

Expression

Mammalian

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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NW50

Plasmid

#133445

Purpose

Covalently circularized nanodisc NW50 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Article

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW50

Expression

Bacterial

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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NW9

Plasmid

#133442

Purpose

Covalently circularized nanodisc NW9 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Article

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW9

Expression

Bacterial

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCSC-ISL1-T2A-LHX3

Plasmid

#90215

Purpose

To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with NGN2, Sox11, FGF2 and two small molecules, forskolin and dorsomorphin.

Depositor

Chun-Li Zhang

Article

Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

Insert

ISL1-T2A-LHX3 (ISL1, LHX3 Human)

Use

Lentiviral

Promoter

CMV

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-Hylight

Plasmid

#193447

Purpose

A fluorescent biosensor for fructose 1,6-bisphosphate (FBP). Changes in FBP concentration strongly correlate with changes in glycolytic flux. (mammalian expression vector)

Depositor

Richard Goodman

Article

Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Hylight Biosensor

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dSaCas9-KRAB-T2A-PuroR

Plasmid

#106249

Purpose

Lentiviral vector with puro selection for expression of S. aureus dCas9-KRAB

Depositor

Charles Gersbach

Article

Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

Insert

dead S. aureus Cas9 KRAB T2A PuroR

Use

CRISPR and Lentiviral

Tags

HA-NLS and NLS-KRAB

Expression

Mammalian

Mutation

D10A and N580A

Promoter

hUbC

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pMLS253

Plasmid

#114271

Purpose

SapTrap donor vector for point mutagenesis; pENTR-221::syntron(loxp:unr-119+)

Depositor

Erik Jorgensen

Article

Schwartz et al MicroPubl Biol. 2018 Jul 29;2018:10.1791

Insert

Cbr-Unc-119

Use

CRISPR and Cre/Lox

Mutation

*please see depositor comment below

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pJFA344C7

Plasmid

#49236

Purpose

TET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cells

Depositor

Keith Joung

Article

Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

Insert

TALE-TET1CD Backbone (TET1 Human, Synthetic)

Use

TALEN; Tale-tet1 expression vector

Promoter

EF1a

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pFZ19

Plasmid

#36184

Purpose

Expression of genes under control of an estrogen-inducible 35S promoter

Depositor

Daniel Voytas

Article

Zhang et al Proc Natl Acad Sci U S A. 2010 Jun 29. 1

Type

Empty backbone

Use

Plant t-dna vector

Expression

Plant

Promoter

XVE_35S (estrogen-inducible)

Available Since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only

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pCSC-NGN2-IRES-GFP-T2A-Sox11

Plasmid

#90214

Purpose

To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with ISL1, LHX3, FGF2 and two small molecules, forskolin and dorsomorphin.

Depositor

Chun-Li Zhang

Article

Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

Insert

NGN2-IRES-GFP-T2A-Sox11 (NEUROG2, Sox11 Human, Mouse)

Use

Lentiviral

Promoter

CMV

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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